Increased peripheral T lymphocyte activation in patients with karyotypically normal spontaneous premature ovarian failure
To determine if soluble interleukin 2 (IL-2) receptor measured in serum by an enzymelinked immunosorbent assay (ELISA) might be useful in managing patients with karyotypically normal spontaneous premature ovarian failure. Prospective, controlled observation. Tertiary care research institution. None....
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| Veröffentlicht in: | Fertility and sterility 1991-06, Vol.55 (6), p.1082-1087 |
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| container_title | Fertility and sterility |
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| creator | Nelson, Lawrence M. Kimzey, Lorene M. Merriam, George R. Fleisher, Thomas A. |
| description | To determine if soluble interleukin 2 (IL-2) receptor measured in serum by an enzymelinked immunosorbent assay (ELISA) might be useful in managing patients with karyotypically normal spontaneous premature ovarian failure.
Prospective, controlled observation.
Tertiary care research institution.
None.
Twenty-four patients with karyotypically normal spontaneous premature ovarian failure comprised the study group. Forty-two healthy men and women comprised the normal reference group.
We measured peripheral T lymphocyte human leukocyte antigen locus DR (HLA-DR) expression and IL-2 receptor expression using monoclonal antibodies and flow cytometry. We measured soluble IL-2 receptor levels in serum using an ELISA.
Consistent with previous findings, our patients had significantly higher HLA-DR expression on peripheral T lymphocytes (5.3 ± 0.46) as compared with controls (3.5 ± 0.34) (mean ± SEM, P < 0.01). Seven patients also had elevated IL-2 receptor expression on peripheral T lymphocytes (P < 0.05). However, soluble IL-2 receptor levels in the serum did not differ significantly from normals.
Patients with karyotypically normal spontaneous premature ovarian failure have a modest increase in peripheral T lymphocyte activation measured by flow cytometry. This degree of activation does not result in increased soluble IL-2 receptor release measured by ELISA. |
| doi_str_mv | 10.1016/S0015-0282(16)54356-8 |
| format | Article |
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Prospective, controlled observation.
Tertiary care research institution.
None.
Twenty-four patients with karyotypically normal spontaneous premature ovarian failure comprised the study group. Forty-two healthy men and women comprised the normal reference group.
We measured peripheral T lymphocyte human leukocyte antigen locus DR (HLA-DR) expression and IL-2 receptor expression using monoclonal antibodies and flow cytometry. We measured soluble IL-2 receptor levels in serum using an ELISA.
Consistent with previous findings, our patients had significantly higher HLA-DR expression on peripheral T lymphocytes (5.3 ± 0.46) as compared with controls (3.5 ± 0.34) (mean ± SEM, P < 0.01). Seven patients also had elevated IL-2 receptor expression on peripheral T lymphocytes (P < 0.05). However, soluble IL-2 receptor levels in the serum did not differ significantly from normals.
Patients with karyotypically normal spontaneous premature ovarian failure have a modest increase in peripheral T lymphocyte activation measured by flow cytometry. This degree of activation does not result in increased soluble IL-2 receptor release measured by ELISA.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(16)54356-8</identifier><identifier>PMID: 2037104</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Autoantibodies - analysis ; Biological and medical sciences ; Birth control ; Cell Nucleus - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Gynecology. Andrology. Obstetrics ; HLA-DR Antigens - analysis ; Humans ; Karyotyping ; Lymphocyte Activation ; Male ; Medical sciences ; Ovarian Diseases - genetics ; Ovarian Diseases - immunology ; Prospective Studies ; Receptors, Interleukin-2 - analysis ; Reference Values ; Sterility. Assisted procreation ; T-Lymphocytes - immunology</subject><ispartof>Fertility and sterility, 1991-06, Vol.55 (6), p.1082-1087</ispartof><rights>1991 American Society for Reproductive Medicine</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-79b0df104577976597e1bba68cc06d7a67c8057f6eb7fa7f06fed150cbd56de83</citedby><cites>FETCH-LOGICAL-c305t-79b0df104577976597e1bba68cc06d7a67c8057f6eb7fa7f06fed150cbd56de83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(16)54356-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19776390$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2037104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nelson, Lawrence M.</creatorcontrib><creatorcontrib>Kimzey, Lorene M.</creatorcontrib><creatorcontrib>Merriam, George R.</creatorcontrib><creatorcontrib>Fleisher, Thomas A.</creatorcontrib><title>Increased peripheral T lymphocyte activation in patients with karyotypically normal spontaneous premature ovarian failure</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To determine if soluble interleukin 2 (IL-2) receptor measured in serum by an enzymelinked immunosorbent assay (ELISA) might be useful in managing patients with karyotypically normal spontaneous premature ovarian failure.
Prospective, controlled observation.
Tertiary care research institution.
None.
Twenty-four patients with karyotypically normal spontaneous premature ovarian failure comprised the study group. Forty-two healthy men and women comprised the normal reference group.
We measured peripheral T lymphocyte human leukocyte antigen locus DR (HLA-DR) expression and IL-2 receptor expression using monoclonal antibodies and flow cytometry. We measured soluble IL-2 receptor levels in serum using an ELISA.
Consistent with previous findings, our patients had significantly higher HLA-DR expression on peripheral T lymphocytes (5.3 ± 0.46) as compared with controls (3.5 ± 0.34) (mean ± SEM, P < 0.01). Seven patients also had elevated IL-2 receptor expression on peripheral T lymphocytes (P < 0.05). However, soluble IL-2 receptor levels in the serum did not differ significantly from normals.
Patients with karyotypically normal spontaneous premature ovarian failure have a modest increase in peripheral T lymphocyte activation measured by flow cytometry. This degree of activation does not result in increased soluble IL-2 receptor release measured by ELISA.</description><subject>Autoantibodies - analysis</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cell Nucleus - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>HLA-DR Antigens - analysis</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Ovarian Diseases - genetics</subject><subject>Ovarian Diseases - immunology</subject><subject>Prospective Studies</subject><subject>Receptors, Interleukin-2 - analysis</subject><subject>Reference Values</subject><subject>Sterility. Assisted procreation</subject><subject>T-Lymphocytes - immunology</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQhS1EVbaFn1DJFxA9BMab2k5OqKpaqFSpB8rZcpyx1pDYxvYuyr_H212VIyfPaN6bef4IuWDwiQETn78DMN7Ault_ZOKSX7VcNN0rsmK8Flzw9jVZvUjekLOcfwKAYHJ9Sk7X0EoGVyuy3HuTUGccacTk4gaTnugTnZY5boJZClJtitvp4oKnztNYK_Ql0z-ubOgvnZZQluiMnqaF-pDmas8x-KI9hm2mMeGsyzYhDTudnPbUajfV_i05sXrK-O74npMfd7dPN9-ah8ev9zfXD41pgZdG9gOMtmblUvZS8F4iGwYtOmNAjFILaTrg0gocpNXSgrA4Mg5mGLkYsWvPyYfD3pjC7y3momaXDU7TIaCq7g5E21YhPwhNCjkntComN9cPKgZqj1w9I1d7nqp2z8jV_sDF8cB2mHF8cR0Z1_n741zniskm7Y3L_5b3Uoq2h6r7ctBhpbFzmFQ2FbXB0SU0RY3B_SfJXy4CoVA</recordid><startdate>199106</startdate><enddate>199106</enddate><creator>Nelson, Lawrence M.</creator><creator>Kimzey, Lorene M.</creator><creator>Merriam, George R.</creator><creator>Fleisher, Thomas A.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199106</creationdate><title>Increased peripheral T lymphocyte activation in patients with karyotypically normal spontaneous premature ovarian failure</title><author>Nelson, Lawrence M. ; Kimzey, Lorene M. ; Merriam, George R. ; Fleisher, Thomas A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-79b0df104577976597e1bba68cc06d7a67c8057f6eb7fa7f06fed150cbd56de83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Autoantibodies - analysis</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cell Nucleus - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>HLA-DR Antigens - analysis</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Lymphocyte Activation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Ovarian Diseases - genetics</topic><topic>Ovarian Diseases - immunology</topic><topic>Prospective Studies</topic><topic>Receptors, Interleukin-2 - analysis</topic><topic>Reference Values</topic><topic>Sterility. Assisted procreation</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nelson, Lawrence M.</creatorcontrib><creatorcontrib>Kimzey, Lorene M.</creatorcontrib><creatorcontrib>Merriam, George R.</creatorcontrib><creatorcontrib>Fleisher, Thomas A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nelson, Lawrence M.</au><au>Kimzey, Lorene M.</au><au>Merriam, George R.</au><au>Fleisher, Thomas A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased peripheral T lymphocyte activation in patients with karyotypically normal spontaneous premature ovarian failure</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>1991-06</date><risdate>1991</risdate><volume>55</volume><issue>6</issue><spage>1082</spage><epage>1087</epage><pages>1082-1087</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To determine if soluble interleukin 2 (IL-2) receptor measured in serum by an enzymelinked immunosorbent assay (ELISA) might be useful in managing patients with karyotypically normal spontaneous premature ovarian failure.
Prospective, controlled observation.
Tertiary care research institution.
None.
Twenty-four patients with karyotypically normal spontaneous premature ovarian failure comprised the study group. Forty-two healthy men and women comprised the normal reference group.
We measured peripheral T lymphocyte human leukocyte antigen locus DR (HLA-DR) expression and IL-2 receptor expression using monoclonal antibodies and flow cytometry. We measured soluble IL-2 receptor levels in serum using an ELISA.
Consistent with previous findings, our patients had significantly higher HLA-DR expression on peripheral T lymphocytes (5.3 ± 0.46) as compared with controls (3.5 ± 0.34) (mean ± SEM, P < 0.01). Seven patients also had elevated IL-2 receptor expression on peripheral T lymphocytes (P < 0.05). However, soluble IL-2 receptor levels in the serum did not differ significantly from normals.
Patients with karyotypically normal spontaneous premature ovarian failure have a modest increase in peripheral T lymphocyte activation measured by flow cytometry. This degree of activation does not result in increased soluble IL-2 receptor release measured by ELISA.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2037104</pmid><doi>10.1016/S0015-0282(16)54356-8</doi><tpages>6</tpages></addata></record> |
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| ispartof | Fertility and sterility, 1991-06, Vol.55 (6), p.1082-1087 |
| issn | 0015-0282 1556-5653 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80580633 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Autoantibodies - analysis Biological and medical sciences Birth control Cell Nucleus - immunology Enzyme-Linked Immunosorbent Assay Female Gynecology. Andrology. Obstetrics HLA-DR Antigens - analysis Humans Karyotyping Lymphocyte Activation Male Medical sciences Ovarian Diseases - genetics Ovarian Diseases - immunology Prospective Studies Receptors, Interleukin-2 - analysis Reference Values Sterility. Assisted procreation T-Lymphocytes - immunology |
| title | Increased peripheral T lymphocyte activation in patients with karyotypically normal spontaneous premature ovarian failure |
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