Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium
In this study the deoxyuridine suppression test (dUST) was performed on isolated rat colonocytes to establish its value as an indicator of folate status in the colonic epithelium. [3H]thymidine incorporation into DNA was suppressed < 90% by deoxyuridine (dU) concentrations < 2.5 µmol/L. Preinc...
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| Veröffentlicht in: | The American journal of clinical nutrition 1991-06, Vol.53 (6), p.1450-1454 |
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| creator | Cravo, ML Mason, JB Selhub, J Rosenberg, IH |
| description | In this study the deoxyuridine suppression test (dUST) was performed on isolated rat colonocytes to establish its value as an indicator of folate status in the colonic epithelium. [3H]thymidine incorporation into DNA was suppressed < 90% by deoxyuridine (dU) concentrations < 2.5 µmol/L. Preincubation of cells with 5-fluorouracil (1–100 µmol/L) but not methotrexate (10–100 µmol) resulted in a significant decrease in the degree of suppression. The dUST performed on colonocytes from folate-deficient animals displayed less suppression than on colonocytes from folate-replete animals (P < 0.05). The abnormal degree of suppression was corrected by adding 100 µmol folinic acid/L. There was a negative correlation between the degree of suppression and the folate concentration of the colonic epithelium (P < 0.001). These data indicate that the dUST is useful for detecting folate deficiency in the colonic epithelium and may therefore be valuable in assessing a deficiency state localized to that epithelium. |
| doi_str_mv | 10.1093/ajcn/53.6.1450 |
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[3H]thymidine incorporation into DNA was suppressed < 90% by deoxyuridine (dU) concentrations < 2.5 µmol/L. Preincubation of cells with 5-fluorouracil (1–100 µmol/L) but not methotrexate (10–100 µmol) resulted in a significant decrease in the degree of suppression. The dUST performed on colonocytes from folate-deficient animals displayed less suppression than on colonocytes from folate-replete animals (P < 0.05). The abnormal degree of suppression was corrected by adding 100 µmol folinic acid/L. There was a negative correlation between the degree of suppression and the folate concentration of the colonic epithelium (P < 0.001). These data indicate that the dUST is useful for detecting folate deficiency in the colonic epithelium and may therefore be valuable in assessing a deficiency state localized to that epithelium.</description><identifier>ISSN: 0002-9165</identifier><identifier>EISSN: 1938-3207</identifier><identifier>DOI: 10.1093/ajcn/53.6.1450</identifier><identifier>PMID: 2035472</identifier><identifier>CODEN: AJCNAC</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>5-fluorouracil ; ACIDE FOLIQUE ; ACIDO FOLICO ; Animals ; ANTIVITAMINAS ; ANTIVITAMINE ; Biological and medical sciences ; CARENCE EN VITAMINE ; Cells, Cultured ; Colon - chemistry ; Colon - cytology ; DEFICIENCIA DE VITAMINAS ; DEOXYRIBONUCLEOSIDE ; Deoxyuridine ; deoxyuridine suppression test ; DESOXIRIBONUCLEOSIDOS ; DNA - metabolism ; EPITELIO ; EPITHELIUM ; Epithelium - chemistry ; FLUOROURACIL ; Fluorouracil - metabolism ; FLUOROURACILE ; Folate ; folate deficiency ; folic acid ; Folic Acid - analysis ; INTESTIN ; INTESTINOS ; Investigative techniques, diagnostic techniques (general aspects) ; localized folate deficiency ; Male ; Medical sciences ; Metabolic diseases ; methotrexate ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; RAT ; rat colonocytes ; RATA ; Rats ; Rats, Inbred Strains ; THYMIDINE ; Thymidine - metabolism ; TIMIDINA</subject><ispartof>The American journal of clinical nutrition, 1991-06, Vol.53 (6), p.1450-1454</ispartof><rights>1991 American Society for Nutrition.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-6b347da3a5767cd819d036af03922c3ee4903e3b385b1734a6c1a6a11aef909e3</citedby><cites>FETCH-LOGICAL-c494t-6b347da3a5767cd819d036af03922c3ee4903e3b385b1734a6c1a6a11aef909e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19739636$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2035472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cravo, ML</creatorcontrib><creatorcontrib>Mason, JB</creatorcontrib><creatorcontrib>Selhub, J</creatorcontrib><creatorcontrib>Rosenberg, IH</creatorcontrib><title>Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium</title><title>The American journal of clinical nutrition</title><addtitle>Am J Clin Nutr</addtitle><description>In this study the deoxyuridine suppression test (dUST) was performed on isolated rat colonocytes to establish its value as an indicator of folate status in the colonic epithelium. [3H]thymidine incorporation into DNA was suppressed < 90% by deoxyuridine (dU) concentrations < 2.5 µmol/L. Preincubation of cells with 5-fluorouracil (1–100 µmol/L) but not methotrexate (10–100 µmol) resulted in a significant decrease in the degree of suppression. The dUST performed on colonocytes from folate-deficient animals displayed less suppression than on colonocytes from folate-replete animals (P < 0.05). The abnormal degree of suppression was corrected by adding 100 µmol folinic acid/L. There was a negative correlation between the degree of suppression and the folate concentration of the colonic epithelium (P < 0.001). These data indicate that the dUST is useful for detecting folate deficiency in the colonic epithelium and may therefore be valuable in assessing a deficiency state localized to that epithelium.</description><subject>5-fluorouracil</subject><subject>ACIDE FOLIQUE</subject><subject>ACIDO FOLICO</subject><subject>Animals</subject><subject>ANTIVITAMINAS</subject><subject>ANTIVITAMINE</subject><subject>Biological and medical sciences</subject><subject>CARENCE EN VITAMINE</subject><subject>Cells, Cultured</subject><subject>Colon - chemistry</subject><subject>Colon - cytology</subject><subject>DEFICIENCIA DE VITAMINAS</subject><subject>DEOXYRIBONUCLEOSIDE</subject><subject>Deoxyuridine</subject><subject>deoxyuridine suppression test</subject><subject>DESOXIRIBONUCLEOSIDOS</subject><subject>DNA - metabolism</subject><subject>EPITELIO</subject><subject>EPITHELIUM</subject><subject>Epithelium - chemistry</subject><subject>FLUOROURACIL</subject><subject>Fluorouracil - metabolism</subject><subject>FLUOROURACILE</subject><subject>Folate</subject><subject>folate deficiency</subject><subject>folic acid</subject><subject>Folic Acid - analysis</subject><subject>INTESTIN</subject><subject>INTESTINOS</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>localized folate deficiency</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>methotrexate</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>RAT</subject><subject>rat colonocytes</subject><subject>RATA</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>THYMIDINE</subject><subject>Thymidine - metabolism</subject><subject>TIMIDINA</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM-L1DAYhoMo67h69SAIueits0m_Jm2OsvgLFjzonMM3yVfN0Glq0i47_vWmzqAnT4G8T968PIy9lGIrhYEbPLjxRsFWb2WjxCO2kQa6CmrRPmYbIURdGanVU_Ys54MQsm46fcWuagGqaesNO-wy8djz-QdxT_HhtKTgw0g8L9OUKOcQRz5TnvkcOd3jsOBMfIgOh_CLPO_jsF546oMLNLoTDyNPOHMXhzgGx2kKpXsIy_E5e9LjkOnF5bxmuw_vv91-qu6-fPx8--6uco1p5krvoWk9AqpWt8530ngBGnsBpq4dEDVGAMEeOrWXLTSonUSNUiL1RhiCa_b23Dul-HMp0-0xZEfDgCPFJdtOqBaMhAJuz6BLMedEvZ1SOGI6WSnsKteucq0Cq-0qtzx4fWle9kfyf_GLzZK_ueSYi6A-4ehC_tdqyr8adOFenbkeo8XvqTC7r0Yq3el1VXcOqTi6D5Rs_mOWfEjkZutj-N--32Y5n4g</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Cravo, ML</creator><creator>Mason, JB</creator><creator>Selhub, J</creator><creator>Rosenberg, IH</creator><general>Elsevier Inc</general><general>American Society for Clinical Nutrition</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910601</creationdate><title>Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium</title><author>Cravo, ML ; Mason, JB ; Selhub, J ; Rosenberg, IH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-6b347da3a5767cd819d036af03922c3ee4903e3b385b1734a6c1a6a11aef909e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>5-fluorouracil</topic><topic>ACIDE FOLIQUE</topic><topic>ACIDO FOLICO</topic><topic>Animals</topic><topic>ANTIVITAMINAS</topic><topic>ANTIVITAMINE</topic><topic>Biological and medical sciences</topic><topic>CARENCE EN VITAMINE</topic><topic>Cells, Cultured</topic><topic>Colon - chemistry</topic><topic>Colon - cytology</topic><topic>DEFICIENCIA DE VITAMINAS</topic><topic>DEOXYRIBONUCLEOSIDE</topic><topic>Deoxyuridine</topic><topic>deoxyuridine suppression test</topic><topic>DESOXIRIBONUCLEOSIDOS</topic><topic>DNA - metabolism</topic><topic>EPITELIO</topic><topic>EPITHELIUM</topic><topic>Epithelium - chemistry</topic><topic>FLUOROURACIL</topic><topic>Fluorouracil - metabolism</topic><topic>FLUOROURACILE</topic><topic>Folate</topic><topic>folate deficiency</topic><topic>folic acid</topic><topic>Folic Acid - analysis</topic><topic>INTESTIN</topic><topic>INTESTINOS</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>localized folate deficiency</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>methotrexate</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>RAT</topic><topic>rat colonocytes</topic><topic>RATA</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>THYMIDINE</topic><topic>Thymidine - metabolism</topic><topic>TIMIDINA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cravo, ML</creatorcontrib><creatorcontrib>Mason, JB</creatorcontrib><creatorcontrib>Selhub, J</creatorcontrib><creatorcontrib>Rosenberg, IH</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cravo, ML</au><au>Mason, JB</au><au>Selhub, J</au><au>Rosenberg, IH</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>53</volume><issue>6</issue><spage>1450</spage><epage>1454</epage><pages>1450-1454</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>In this study the deoxyuridine suppression test (dUST) was performed on isolated rat colonocytes to establish its value as an indicator of folate status in the colonic epithelium. [3H]thymidine incorporation into DNA was suppressed < 90% by deoxyuridine (dU) concentrations < 2.5 µmol/L. Preincubation of cells with 5-fluorouracil (1–100 µmol/L) but not methotrexate (10–100 µmol) resulted in a significant decrease in the degree of suppression. The dUST performed on colonocytes from folate-deficient animals displayed less suppression than on colonocytes from folate-replete animals (P < 0.05). The abnormal degree of suppression was corrected by adding 100 µmol folinic acid/L. There was a negative correlation between the degree of suppression and the folate concentration of the colonic epithelium (P < 0.001). These data indicate that the dUST is useful for detecting folate deficiency in the colonic epithelium and may therefore be valuable in assessing a deficiency state localized to that epithelium.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2035472</pmid><doi>10.1093/ajcn/53.6.1450</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 5-fluorouracil ACIDE FOLIQUE ACIDO FOLICO Animals ANTIVITAMINAS ANTIVITAMINE Biological and medical sciences CARENCE EN VITAMINE Cells, Cultured Colon - chemistry Colon - cytology DEFICIENCIA DE VITAMINAS DEOXYRIBONUCLEOSIDE Deoxyuridine deoxyuridine suppression test DESOXIRIBONUCLEOSIDOS DNA - metabolism EPITELIO EPITHELIUM Epithelium - chemistry FLUOROURACIL Fluorouracil - metabolism FLUOROURACILE Folate folate deficiency folic acid Folic Acid - analysis INTESTIN INTESTINOS Investigative techniques, diagnostic techniques (general aspects) localized folate deficiency Male Medical sciences Metabolic diseases methotrexate Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques RAT rat colonocytes RATA Rats Rats, Inbred Strains THYMIDINE Thymidine - metabolism TIMIDINA |
| title | Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium |
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