Bovine monoclonal anti-idiotypes induce antibodies specific for a synthetic peptide bearing a neutralizing epitope of bovine herpesvirus 1 glycoprotein gI (gB)
Bovine monoclonal anti-Id mimicking a neutralizing epitope of bovine herpesvirus-1 (BHV-1) glycoprotein gI were developed. An epitope present on the 74K subunit of gI identified by a murine mAb 1E11 was selected for this study. Bovine lymphocytes from the prefemoral lymph node of a heifer immunized...
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| Veröffentlicht in: | The Journal of immunology (1950) 1991-05, Vol.146 (10), p.3489-3495 |
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| container_title | The Journal of immunology (1950) |
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| creator | Hariharan, K. (University of Nebraska, Lincoln, NE) Hariharan, M.J Zamb, T.J Krueger, R.J Srikumaran, S |
| description | Bovine monoclonal anti-Id mimicking a neutralizing epitope of bovine herpesvirus-1 (BHV-1) glycoprotein gI were developed. An epitope present on the 74K subunit of gI identified by a murine mAb 1E11 was selected for this study. Bovine lymphocytes from the prefemoral lymph node of a heifer immunized with mAb 1E11 were fused with SP-2/0, a nonsecreting murine cell-line. Two bovine x murine hybridomas secreting bovine monoclonal anti- Id specific for the Id of 1E11 were stabilized. These anti-Id inhibited the binding of 1E11 to purified glycoprotein gI in a dose-dependent fashion. Naive mice immunized with the anti-Id produced anti-anti- Id (Ab3) that reacted with BHV-1 glycoprotein gI in a RIA, and neutralized BHV-1 infection in vitro. The Ab3 also showed reactivity to the 74K subunit of authentic gI glycoprotein in a Western blot analysis, and to the synthetic peptide bearing the 1E11 epitope in a RIA. These results substantiate the presence of the population of anti-Ab2 that functionally resemble antibodies specific for the immunizing Ag BHV-1 in Ab3, and demonstrate the ability of these anti-Id to elicit BHV-1-specific antibody response |
| doi_str_mv | 10.4049/jimmunol.146.10.3489 |
| format | Article |
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(University of Nebraska, Lincoln, NE) ; Hariharan, M.J ; Zamb, T.J ; Krueger, R.J ; Srikumaran, S</creator><creatorcontrib>Hariharan, K. (University of Nebraska, Lincoln, NE) ; Hariharan, M.J ; Zamb, T.J ; Krueger, R.J ; Srikumaran, S</creatorcontrib><description>Bovine monoclonal anti-Id mimicking a neutralizing epitope of bovine herpesvirus-1 (BHV-1) glycoprotein gI were developed. An epitope present on the 74K subunit of gI identified by a murine mAb 1E11 was selected for this study. Bovine lymphocytes from the prefemoral lymph node of a heifer immunized with mAb 1E11 were fused with SP-2/0, a nonsecreting murine cell-line. Two bovine x murine hybridomas secreting bovine monoclonal anti- Id specific for the Id of 1E11 were stabilized. These anti-Id inhibited the binding of 1E11 to purified glycoprotein gI in a dose-dependent fashion. Naive mice immunized with the anti-Id produced anti-anti- Id (Ab3) that reacted with BHV-1 glycoprotein gI in a RIA, and neutralized BHV-1 infection in vitro. The Ab3 also showed reactivity to the 74K subunit of authentic gI glycoprotein in a Western blot analysis, and to the synthetic peptide bearing the 1E11 epitope in a RIA. These results substantiate the presence of the population of anti-Ab2 that functionally resemble antibodies specific for the immunizing Ag BHV-1 in Ab3, and demonstrate the ability of these anti-Id to elicit BHV-1-specific antibody response</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.146.10.3489</identifier><identifier>PMID: 1709196</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - biosynthesis ; Antibody production ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGENE ; ANTIGENOS ; Biological and medical sciences ; Cattle ; Epitopes ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; GLICOPROTEINAS ; GLYCOPROTEINE ; Herpesvirus 1, Bovine - immunology ; HERPESVIRUS BOVIN ; HERPESVIRUS BOVINO ; Immunobiology ; Immunoglobulin Idiotypes - immunology ; INHIBICION ; INHIBITION ; Mice ; Mice, Inbred BALB C ; Peptide Fragments - immunology ; REPONSE IMMUNITAIRE ; RESPUESTA INMUNOLOGICA ; Viral Proteins - immunology</subject><ispartof>The Journal of immunology (1950), 1991-05, Vol.146 (10), p.3489-3495</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3759-9f7bd8daeec10081d3bd5ba61b1dfc537ff22d98d34417e792f9106b799755153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4940204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1709196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hariharan, K. (University of Nebraska, Lincoln, NE)</creatorcontrib><creatorcontrib>Hariharan, M.J</creatorcontrib><creatorcontrib>Zamb, T.J</creatorcontrib><creatorcontrib>Krueger, R.J</creatorcontrib><creatorcontrib>Srikumaran, S</creatorcontrib><title>Bovine monoclonal anti-idiotypes induce antibodies specific for a synthetic peptide bearing a neutralizing epitope of bovine herpesvirus 1 glycoprotein gI (gB)</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Bovine monoclonal anti-Id mimicking a neutralizing epitope of bovine herpesvirus-1 (BHV-1) glycoprotein gI were developed. An epitope present on the 74K subunit of gI identified by a murine mAb 1E11 was selected for this study. Bovine lymphocytes from the prefemoral lymph node of a heifer immunized with mAb 1E11 were fused with SP-2/0, a nonsecreting murine cell-line. Two bovine x murine hybridomas secreting bovine monoclonal anti- Id specific for the Id of 1E11 were stabilized. These anti-Id inhibited the binding of 1E11 to purified glycoprotein gI in a dose-dependent fashion. Naive mice immunized with the anti-Id produced anti-anti- Id (Ab3) that reacted with BHV-1 glycoprotein gI in a RIA, and neutralized BHV-1 infection in vitro. The Ab3 also showed reactivity to the 74K subunit of authentic gI glycoprotein in a Western blot analysis, and to the synthetic peptide bearing the 1E11 epitope in a RIA. These results substantiate the presence of the population of anti-Ab2 that functionally resemble antibodies specific for the immunizing Ag BHV-1 in Ab3, and demonstrate the ability of these anti-Id to elicit BHV-1-specific antibody response</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibody production</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Epitopes</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>Herpesvirus 1, Bovine - immunology</subject><subject>HERPESVIRUS BOVIN</subject><subject>HERPESVIRUS BOVINO</subject><subject>Immunobiology</subject><subject>Immunoglobulin Idiotypes - immunology</subject><subject>INHIBICION</subject><subject>INHIBITION</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Peptide Fragments - immunology</subject><subject>REPONSE IMMUNITAIRE</subject><subject>RESPUESTA INMUNOLOGICA</subject><subject>Viral Proteins - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEKkPhBRBIXiBUFhnsxLHjJa34qVSJBXQdOfZ1xpVjBzvpaHgZXhUPGUCsWFn33O-eI_kUxQuCtxRT8fbOjuPig9sSyrZZrGkrHhQb0jS4ZAyzh8UG46oqCWf8cfEkpTuMMcMVPSvOCMeCCLYpflyGe-sBjcEH5YKXDkk_29JqG-bDBAlZrxcFv9Q-aJuVNIGyxipkQkQSpYOfdzDneYJpthpQDzJaP-Sdh2WO0tnvxxEmO4cJUDCoX1N3EHPEvY1LQgQN7qDCFMMM1qPhGl0Ml2-eFo-MdAmend7z4vbD-69Xn8qbzx-vr97dlKrmjSiF4b1utQRQBOOW6LrXTS8Z6Yk2qqm5MVWlRatrSgkHLiojCGY9F4I3DWnq8-L16pvzvy2Q5m60SYFz0kNYUtfihrb5J_8LEoYxF7TOIF1BFUNKEUw3RTvKeOgI7o4Fdr8L7HKBR_FYYD57efJf-hH036O1sbx_ddrLpKQzUXpl0x-MCoorTDN2sWI7O-z2NkKXRulcNiXdfr__N_H5ihoZOjnE7Hb7RZCaVbyufwKT7L7p</recordid><startdate>19910515</startdate><enddate>19910515</enddate><creator>Hariharan, K. (University of Nebraska, Lincoln, NE)</creator><creator>Hariharan, M.J</creator><creator>Zamb, T.J</creator><creator>Krueger, R.J</creator><creator>Srikumaran, S</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910515</creationdate><title>Bovine monoclonal anti-idiotypes induce antibodies specific for a synthetic peptide bearing a neutralizing epitope of bovine herpesvirus 1 glycoprotein gI (gB)</title><author>Hariharan, K. (University of Nebraska, Lincoln, NE) ; Hariharan, M.J ; Zamb, T.J ; Krueger, R.J ; Srikumaran, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3759-9f7bd8daeec10081d3bd5ba61b1dfc537ff22d98d34417e792f9106b799755153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibody production</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Epitopes</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>Herpesvirus 1, Bovine - immunology</topic><topic>HERPESVIRUS BOVIN</topic><topic>HERPESVIRUS BOVINO</topic><topic>Immunobiology</topic><topic>Immunoglobulin Idiotypes - immunology</topic><topic>INHIBICION</topic><topic>INHIBITION</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Peptide Fragments - immunology</topic><topic>REPONSE IMMUNITAIRE</topic><topic>RESPUESTA INMUNOLOGICA</topic><topic>Viral Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hariharan, K. (University of Nebraska, Lincoln, NE)</creatorcontrib><creatorcontrib>Hariharan, M.J</creatorcontrib><creatorcontrib>Zamb, T.J</creatorcontrib><creatorcontrib>Krueger, R.J</creatorcontrib><creatorcontrib>Srikumaran, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hariharan, K. (University of Nebraska, Lincoln, NE)</au><au>Hariharan, M.J</au><au>Zamb, T.J</au><au>Krueger, R.J</au><au>Srikumaran, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine monoclonal anti-idiotypes induce antibodies specific for a synthetic peptide bearing a neutralizing epitope of bovine herpesvirus 1 glycoprotein gI (gB)</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1991-05-15</date><risdate>1991</risdate><volume>146</volume><issue>10</issue><spage>3489</spage><epage>3495</epage><pages>3489-3495</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>Bovine monoclonal anti-Id mimicking a neutralizing epitope of bovine herpesvirus-1 (BHV-1) glycoprotein gI were developed. An epitope present on the 74K subunit of gI identified by a murine mAb 1E11 was selected for this study. Bovine lymphocytes from the prefemoral lymph node of a heifer immunized with mAb 1E11 were fused with SP-2/0, a nonsecreting murine cell-line. Two bovine x murine hybridomas secreting bovine monoclonal anti- Id specific for the Id of 1E11 were stabilized. These anti-Id inhibited the binding of 1E11 to purified glycoprotein gI in a dose-dependent fashion. Naive mice immunized with the anti-Id produced anti-anti- Id (Ab3) that reacted with BHV-1 glycoprotein gI in a RIA, and neutralized BHV-1 infection in vitro. The Ab3 also showed reactivity to the 74K subunit of authentic gI glycoprotein in a Western blot analysis, and to the synthetic peptide bearing the 1E11 epitope in a RIA. These results substantiate the presence of the population of anti-Ab2 that functionally resemble antibodies specific for the immunizing Ag BHV-1 in Ab3, and demonstrate the ability of these anti-Id to elicit BHV-1-specific antibody response</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>1709196</pmid><doi>10.4049/jimmunol.146.10.3489</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1991-05, Vol.146 (10), p.3489-3495 |
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| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Viral - biosynthesis Antibody production ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGENE ANTIGENOS Biological and medical sciences Cattle Epitopes Female Fundamental and applied biological sciences. Psychology Fundamental immunology GLICOPROTEINAS GLYCOPROTEINE Herpesvirus 1, Bovine - immunology HERPESVIRUS BOVIN HERPESVIRUS BOVINO Immunobiology Immunoglobulin Idiotypes - immunology INHIBICION INHIBITION Mice Mice, Inbred BALB C Peptide Fragments - immunology REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA Viral Proteins - immunology |
| title | Bovine monoclonal anti-idiotypes induce antibodies specific for a synthetic peptide bearing a neutralizing epitope of bovine herpesvirus 1 glycoprotein gI (gB) |
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