Sustained isometric contraction of skeletal muscle results in release of immunoreactive neurokinins in the spinal cord of the anaesthetized cat
Antibody microprobes were used to study release of immunoreactive neurokinins in the dorsal horn of the anaesthetized spinal cat following sustained isometric contraction of ipsilateral hindlimb muscles. Microprobes had immobilized antibodies to neurokinin A (NKA) on their outer surfaces and bound a...
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| Veröffentlicht in: | Neuroscience letters 1991-01, Vol.122 (2), p.191-194 |
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| container_title | Neuroscience letters |
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| creator | Duggan, A.W. Hope, P.J. Lang, C.W. Williams, C.A. |
| description | Antibody microprobes were used to study release of immunoreactive neurokinins in the dorsal horn of the anaesthetized spinal cat following sustained isometric contraction of ipsilateral hindlimb muscles. Microprobes had immobilized antibodies to neurokinin A (NKA) on their outer surfaces and bound a proportion of released molecules when inserted in the central nervous system. Bound molecules were detected in autoradiographs as zones of reduced binding of
125I-NKA in which microprobes were incubated after withdrawal from the spinal cord. The left hindlimb was immobilized using an epoxy bandage splint and isometric contraction of muscles induced by intermittent tetanic stimulation of a ventral root. A basal presence of immunoreactive neurokinins was detected and this was increased by sustained isometric muscle contraction. It is probable that ergoreceptors contain and release neurokinins. |
| doi_str_mv | 10.1016/0304-3940(91)90855-N |
| format | Article |
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125I-NKA in which microprobes were incubated after withdrawal from the spinal cord. The left hindlimb was immobilized using an epoxy bandage splint and isometric contraction of muscles induced by intermittent tetanic stimulation of a ventral root. A basal presence of immunoreactive neurokinins was detected and this was increased by sustained isometric muscle contraction. It is probable that ergoreceptors contain and release neurokinins.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(91)90855-N</identifier><identifier>PMID: 1851262</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Anesthesia, General ; Animals ; Antibody microprobe ; Biological and medical sciences ; Cats ; Electric Stimulation ; Fundamental and applied biological sciences. Psychology ; Hindlimb - innervation ; Immunoassay ; Isometric Contraction ; Isometric muscle contraction ; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration ; Muscles - innervation ; Neurokinin A - analysis ; Neurokinin A - metabolism ; Neurokinin release ; Spinal cord ; Spinal Cord - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1991-01, Vol.122 (2), p.191-194</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-5cfc6437896ebc9639073b78805210e22ca6f0969f5cc92292664465e0b1f6a93</citedby><cites>FETCH-LOGICAL-c418t-5cfc6437896ebc9639073b78805210e22ca6f0969f5cc92292664465e0b1f6a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3940(91)90855-N$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19536426$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1851262$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duggan, A.W.</creatorcontrib><creatorcontrib>Hope, P.J.</creatorcontrib><creatorcontrib>Lang, C.W.</creatorcontrib><creatorcontrib>Williams, C.A.</creatorcontrib><title>Sustained isometric contraction of skeletal muscle results in release of immunoreactive neurokinins in the spinal cord of the anaesthetized cat</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Antibody microprobes were used to study release of immunoreactive neurokinins in the dorsal horn of the anaesthetized spinal cat following sustained isometric contraction of ipsilateral hindlimb muscles. Microprobes had immobilized antibodies to neurokinin A (NKA) on their outer surfaces and bound a proportion of released molecules when inserted in the central nervous system. Bound molecules were detected in autoradiographs as zones of reduced binding of
125I-NKA in which microprobes were incubated after withdrawal from the spinal cord. The left hindlimb was immobilized using an epoxy bandage splint and isometric contraction of muscles induced by intermittent tetanic stimulation of a ventral root. A basal presence of immunoreactive neurokinins was detected and this was increased by sustained isometric muscle contraction. It is probable that ergoreceptors contain and release neurokinins.</description><subject>Anesthesia, General</subject><subject>Animals</subject><subject>Antibody microprobe</subject><subject>Biological and medical sciences</subject><subject>Cats</subject><subject>Electric Stimulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hindlimb - innervation</subject><subject>Immunoassay</subject><subject>Isometric Contraction</subject><subject>Isometric muscle contraction</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Muscles - innervation</subject><subject>Neurokinin A - analysis</subject><subject>Neurokinin A - metabolism</subject><subject>Neurokinin release</subject><subject>Spinal cord</subject><subject>Spinal Cord - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vFSEUxYmxqc-n30CT2djoYhQYYGBjYhr_JU1dqGvCu3MnYmfgCUwT-yX6lWX6XuxOV9xwf-dwwiHkGaOvGWXqDe2oaDsj6EvDXhmqpWwvH5AN0z1ve9Pzh2TzF3lEHuf8k1IqmRSn5JRpybjiG3L7dcnF-YBD43OcsSQPDcRQkoPiY2ji2OQrnLC4qZmXDBM2CfMyldz4UMcJXcaV8vO8hJhw1V1jE3BJ8coHH-7A8gObvPehukBMwypYr1xwmOtQ_E1NAK48ISejmzI-PZ5b8v3D-2_nn9qLLx8_n7-7aEEwXVoJIyjR9doo3IFRnaF9t-u1ppIzipyDUyM1yowSwHBuuFJCKIl0x0blTLclZwfffYq_lprBzj4DTpMLGJdsq5FQvdH_BZnUWvKaYEvEAYQUc0442n3ys0u_LaN2Lcyubdi1DWuYvSvMXlbZ86P_sptxuBcdGqr7F8e9y-CmMbkAPt9jRnZK8PX5twcO669de0w2g8cAOPiEUOwQ_b-D_AEjfLQm</recordid><startdate>19910128</startdate><enddate>19910128</enddate><creator>Duggan, A.W.</creator><creator>Hope, P.J.</creator><creator>Lang, C.W.</creator><creator>Williams, C.A.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19910128</creationdate><title>Sustained isometric contraction of skeletal muscle results in release of immunoreactive neurokinins in the spinal cord of the anaesthetized cat</title><author>Duggan, A.W. ; Hope, P.J. ; Lang, C.W. ; Williams, C.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-5cfc6437896ebc9639073b78805210e22ca6f0969f5cc92292664465e0b1f6a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Anesthesia, General</topic><topic>Animals</topic><topic>Antibody microprobe</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Electric Stimulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hindlimb - innervation</topic><topic>Immunoassay</topic><topic>Isometric Contraction</topic><topic>Isometric muscle contraction</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Muscles - innervation</topic><topic>Neurokinin A - analysis</topic><topic>Neurokinin A - metabolism</topic><topic>Neurokinin release</topic><topic>Spinal cord</topic><topic>Spinal Cord - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duggan, A.W.</creatorcontrib><creatorcontrib>Hope, P.J.</creatorcontrib><creatorcontrib>Lang, C.W.</creatorcontrib><creatorcontrib>Williams, C.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duggan, A.W.</au><au>Hope, P.J.</au><au>Lang, C.W.</au><au>Williams, C.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sustained isometric contraction of skeletal muscle results in release of immunoreactive neurokinins in the spinal cord of the anaesthetized cat</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1991-01-28</date><risdate>1991</risdate><volume>122</volume><issue>2</issue><spage>191</spage><epage>194</epage><pages>191-194</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Antibody microprobes were used to study release of immunoreactive neurokinins in the dorsal horn of the anaesthetized spinal cat following sustained isometric contraction of ipsilateral hindlimb muscles. Microprobes had immobilized antibodies to neurokinin A (NKA) on their outer surfaces and bound a proportion of released molecules when inserted in the central nervous system. Bound molecules were detected in autoradiographs as zones of reduced binding of
125I-NKA in which microprobes were incubated after withdrawal from the spinal cord. The left hindlimb was immobilized using an epoxy bandage splint and isometric contraction of muscles induced by intermittent tetanic stimulation of a ventral root. A basal presence of immunoreactive neurokinins was detected and this was increased by sustained isometric muscle contraction. It is probable that ergoreceptors contain and release neurokinins.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>1851262</pmid><doi>10.1016/0304-3940(91)90855-N</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1991-01, Vol.122 (2), p.191-194 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80546798 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Anesthesia, General Animals Antibody microprobe Biological and medical sciences Cats Electric Stimulation Fundamental and applied biological sciences. Psychology Hindlimb - innervation Immunoassay Isometric Contraction Isometric muscle contraction Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Muscles - innervation Neurokinin A - analysis Neurokinin A - metabolism Neurokinin release Spinal cord Spinal Cord - physiology Vertebrates: nervous system and sense organs |
| title | Sustained isometric contraction of skeletal muscle results in release of immunoreactive neurokinins in the spinal cord of the anaesthetized cat |
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