Replication of derivatives of the broad host range plasmid RK2 in two distantly related bacteria

A 0.7-kb segment of the broad host range plasmid RK2 containing the replication origin of this plasmid will replicate in Escherichia coli and Pseudomonas putida when this segment is joined to a 1.8-kb region of RK2 designated traA *. The presence of another region of RK2, designated trf B, that previously was implicated in RK2 replication had no effect on the maintenance of the RK2 trf A *-oriV replicon in these two organisms. These observations indicate a requirement for a minimal amount of information for replication of this broad host range plasmid in two distantly related bacteria.