Structure of the pig insulin dimer in the cubic crystal
Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to...
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| Veröffentlicht in: | Acta crystallographica. Section B, Structural science Structural science, 1991-02, Vol.47 (1), p.127-136 |
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| container_title | Acta crystallographica. Section B, Structural science |
| container_volume | 47 |
| creator | Badger, J. Haris, M. R. Reynolds, C. D. Evans, A. C. Dodson, E. J. Dodson, G. G. North, A. C. T. |
| description | Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to standard values and the estimated error in the positions of well-ordered atoms is about 0.1 A. Residues directly involved in the formation of the exact (crystallographic) cubic insulin dimer are oriented similarly to those in the non-crystallographic 2Zn insulin dimer. Other residues, which make different molecular contacts in the different crystal forms, have locally altered conformations. The cubic insulin molecule is significantly more similar to one of the two independent molecules in the 2Zn insulin dimer than the other. This more similar molecule is expected to be the more stable conformer. |
| doi_str_mv | 10.1107/S0108768190009570 |
| format | Article |
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R. ; Reynolds, C. D. ; Evans, A. C. ; Dodson, E. J. ; Dodson, G. G. ; North, A. C. T.</creator><creatorcontrib>Badger, J. ; Haris, M. R. ; Reynolds, C. D. ; Evans, A. C. ; Dodson, E. J. ; Dodson, G. G. ; North, A. C. T.</creatorcontrib><description>Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to standard values and the estimated error in the positions of well-ordered atoms is about 0.1 A. Residues directly involved in the formation of the exact (crystallographic) cubic insulin dimer are oriented similarly to those in the non-crystallographic 2Zn insulin dimer. Other residues, which make different molecular contacts in the different crystal forms, have locally altered conformations. The cubic insulin molecule is significantly more similar to one of the two independent molecules in the 2Zn insulin dimer than the other. This more similar molecule is expected to be the more stable conformer.</description><identifier>ISSN: 0108-7681</identifier><identifier>EISSN: 1600-5740</identifier><identifier>DOI: 10.1107/S0108768190009570</identifier><identifier>PMID: 2025410</identifier><identifier>CODEN: ASBSDK</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: International Union of Crystallography</publisher><subject>Animals ; Biological and medical sciences ; Crystalline structure ; Fundamental and applied biological sciences. Psychology ; Insulin - chemistry ; Macromolecular Substances ; Models, Molecular ; Molecular biophysics ; Protein Conformation ; Structure in molecular biology ; Swine ; Thermodynamics ; X-Ray Diffraction - methods</subject><ispartof>Acta crystallographica. 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C.</creatorcontrib><creatorcontrib>Dodson, E. J.</creatorcontrib><creatorcontrib>Dodson, G. G.</creatorcontrib><creatorcontrib>North, A. C. T.</creatorcontrib><title>Structure of the pig insulin dimer in the cubic crystal</title><title>Acta crystallographica. Section B, Structural science</title><addtitle>Acta Cryst. B</addtitle><description>Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to standard values and the estimated error in the positions of well-ordered atoms is about 0.1 A. Residues directly involved in the formation of the exact (crystallographic) cubic insulin dimer are oriented similarly to those in the non-crystallographic 2Zn insulin dimer. Other residues, which make different molecular contacts in the different crystal forms, have locally altered conformations. The cubic insulin molecule is significantly more similar to one of the two independent molecules in the 2Zn insulin dimer than the other. This more similar molecule is expected to be the more stable conformer.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Crystalline structure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insulin - chemistry</subject><subject>Macromolecular Substances</subject><subject>Models, Molecular</subject><subject>Molecular biophysics</subject><subject>Protein Conformation</subject><subject>Structure in molecular biology</subject><subject>Swine</subject><subject>Thermodynamics</subject><subject>X-Ray Diffraction - methods</subject><issn>0108-7681</issn><issn>1600-5740</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMotV4ewIUwC3E3enKZJLPUoq1QFLwgrkImTTQ67dRkBu3bm9rSjQtXIfzfd07yI3SE4QxjEOcPgEEKLnEJAGUhYAv1MQfIC8FgG_WXcb7Md9FejO8JYlhCD_UIkIJh6CPx0IbOtF2wWeOy9s1mc_-a-Vnsaj_LJn5qQ7r9BqarvMlMWMRW1wdox-k62sP1uY-erq8eB6N8fDe8GVyMc8MkIblzWDNeECwmVHNduariaa80RpcMuCaGc20LAtaVhFJZVZhhUTg7YentWtB9dLqaOw_NZ2djq6Y-GlvXemabLioJBWNASQLxCjShiTFYp-bBT3VYKAxqWZb6U1ZyjtfDu2pqJxtj3U7KT9a5jkbXLuiZ8XGDsZLL9MmEyRX25Wu7-H-vuni5HN4Dxks1X6k-tvZ7o-rwobigolDPt0PFHiljIzZWlP4A0KOOEw</recordid><startdate>199102</startdate><enddate>199102</enddate><creator>Badger, J.</creator><creator>Haris, M. R.</creator><creator>Reynolds, C. D.</creator><creator>Evans, A. C.</creator><creator>Dodson, E. J.</creator><creator>Dodson, G. G.</creator><creator>North, A. C. T.</creator><general>International Union of Crystallography</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199102</creationdate><title>Structure of the pig insulin dimer in the cubic crystal</title><author>Badger, J. ; Haris, M. R. ; Reynolds, C. D. ; Evans, A. C. ; Dodson, E. J. ; Dodson, G. G. ; North, A. C. T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4822-ff1a465217d3a6abfbb64108cca9406a2c66ae520ef92338bb14175fed4010a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Crystalline structure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insulin - chemistry</topic><topic>Macromolecular Substances</topic><topic>Models, Molecular</topic><topic>Molecular biophysics</topic><topic>Protein Conformation</topic><topic>Structure in molecular biology</topic><topic>Swine</topic><topic>Thermodynamics</topic><topic>X-Ray Diffraction - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Badger, J.</creatorcontrib><creatorcontrib>Haris, M. R.</creatorcontrib><creatorcontrib>Reynolds, C. D.</creatorcontrib><creatorcontrib>Evans, A. C.</creatorcontrib><creatorcontrib>Dodson, E. J.</creatorcontrib><creatorcontrib>Dodson, G. G.</creatorcontrib><creatorcontrib>North, A. C. T.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta crystallographica. Section B, Structural science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Badger, J.</au><au>Haris, M. R.</au><au>Reynolds, C. D.</au><au>Evans, A. C.</au><au>Dodson, E. J.</au><au>Dodson, G. G.</au><au>North, A. C. T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of the pig insulin dimer in the cubic crystal</atitle><jtitle>Acta crystallographica. Section B, Structural science</jtitle><addtitle>Acta Cryst. B</addtitle><date>1991-02</date><risdate>1991</risdate><volume>47</volume><issue>1</issue><spage>127</spage><epage>136</epage><pages>127-136</pages><issn>0108-7681</issn><eissn>1600-5740</eissn><coden>ASBSDK</coden><abstract>Atomic coordinates for pig insulin in the cubic crystal have been refined by reciprocal-space methods to an R factor of 0.173 for data between 10.0 and 1.7 A resolution with structure-factor amplitudes greater than two standard deviations. Stereochemical parameters for the refined model are close to standard values and the estimated error in the positions of well-ordered atoms is about 0.1 A. Residues directly involved in the formation of the exact (crystallographic) cubic insulin dimer are oriented similarly to those in the non-crystallographic 2Zn insulin dimer. Other residues, which make different molecular contacts in the different crystal forms, have locally altered conformations. The cubic insulin molecule is significantly more similar to one of the two independent molecules in the 2Zn insulin dimer than the other. This more similar molecule is expected to be the more stable conformer.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>International Union of Crystallography</pub><pmid>2025410</pmid><doi>10.1107/S0108768190009570</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0108-7681 |
| ispartof | Acta crystallographica. Section B, Structural science, 1991-02, Vol.47 (1), p.127-136 |
| issn | 0108-7681 1600-5740 |
| language | eng |
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| source | MEDLINE; Crystallography Journals Online |
| subjects | Animals Biological and medical sciences Crystalline structure Fundamental and applied biological sciences. Psychology Insulin - chemistry Macromolecular Substances Models, Molecular Molecular biophysics Protein Conformation Structure in molecular biology Swine Thermodynamics X-Ray Diffraction - methods |
| title | Structure of the pig insulin dimer in the cubic crystal |
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