N-acetyl-β-endorphin1-31 antagonizes the suppressive effect of β-endorphin1-31 on murine splenocyte proliferation via a naloxone-resistant receptor
High affinity binding sites for beta-endorphin1-31 (beta-EP) have been observed on transformed mononuclear cells such as the human U937 monocyte-like cell line and the murine EL4-thymoma line, and on normal murine splenocytes. Binding of beta-EP at these sites is resistant to competition by naloxone...
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Veröffentlicht in: | Biochemical and biophysical research communications 1991-03, Vol.175 (3), p.936-942 |
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description | High affinity binding sites for beta-endorphin1-31 (beta-EP) have been observed on transformed mononuclear cells such as the human U937 monocyte-like cell line and the murine EL4-thymoma line, and on normal murine splenocytes. Binding of beta-EP at these sites is resistant to competition by naloxone and other opiate receptor ligands but sensitive to N-acetyl-beta-endorphin1-31 (N-Ac), cations and GTP-gamma-sulfate. Thus, the following studies were done to determine the functional significance of binding beta-EP and N-Ac. beta-EP suppressed phytohemagglutinin (PHA)-stimulated [3H]thymidine uptake in a dose-dependent, naloxone-insensitive fashion. beta-Endorphin1-27, (des)-tyrosine beta-endorphin2-31, or N-Ac failed to duplicate the suppressive effect of beta-EP. However, N-Ac, which is equipotent to beta-EP at displacing 125I-beta-EP bound to murine splenocytes or U937 cells, antagonized the suppressive effect of beta-EP. Taken together with previous binding studies, the present observations suggest that beta-EP effects receptor-mediated responses on normal immunocytes that do not depend on the activation of neuronal-like opiate receptors which are naloxone-sensitive. N-Ac, which shows minimal binding to such brain opiate receptors, is a potent functional antagonist of the naloxone-insensitive immunocyte receptor for beta-EP. |
doi_str_mv | 10.1016/0006-291X(91)91655-V |
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A ; BURTNESS, M. Z ; SHARP, B. M</creator><creatorcontrib>SHAHABI, N. A ; BURTNESS, M. Z ; SHARP, B. M</creatorcontrib><description>High affinity binding sites for beta-endorphin1-31 (beta-EP) have been observed on transformed mononuclear cells such as the human U937 monocyte-like cell line and the murine EL4-thymoma line, and on normal murine splenocytes. Binding of beta-EP at these sites is resistant to competition by naloxone and other opiate receptor ligands but sensitive to N-acetyl-beta-endorphin1-31 (N-Ac), cations and GTP-gamma-sulfate. Thus, the following studies were done to determine the functional significance of binding beta-EP and N-Ac. beta-EP suppressed phytohemagglutinin (PHA)-stimulated [3H]thymidine uptake in a dose-dependent, naloxone-insensitive fashion. beta-Endorphin1-27, (des)-tyrosine beta-endorphin2-31, or N-Ac failed to duplicate the suppressive effect of beta-EP. However, N-Ac, which is equipotent to beta-EP at displacing 125I-beta-EP bound to murine splenocytes or U937 cells, antagonized the suppressive effect of beta-EP. Taken together with previous binding studies, the present observations suggest that beta-EP effects receptor-mediated responses on normal immunocytes that do not depend on the activation of neuronal-like opiate receptors which are naloxone-sensitive. N-Ac, which shows minimal binding to such brain opiate receptors, is a potent functional antagonist of the naloxone-insensitive immunocyte receptor for beta-EP.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(91)91655-V</identifier><identifier>PMID: 1850996</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Animals ; beta-Endorphin - analogs & derivatives ; beta-Endorphin - antagonists & inhibitors ; beta-Endorphin - pharmacology ; Biological and medical sciences ; Cells, Cultured ; DNA Replication - drug effects ; Female ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Lymphocyte Activation - drug effects ; Lymphocytes - drug effects ; Lymphocytes - immunology ; Mice ; Mice, Inbred Strains ; Naloxone - pharmacology ; Peptide Fragments - antagonists & inhibitors ; Peptide Fragments - pharmacology ; Phytohemagglutinins ; Proteins ; Receptors, Opioid - drug effects ; Receptors, Opioid - physiology ; Spleen ; Thymidine - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1991-03, Vol.175 (3), p.936-942</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-67698444cd341a1b4bc345f8c88bc4c90482803b741e92f9d993f4d0c6a911643</citedby><cites>FETCH-LOGICAL-c332t-67698444cd341a1b4bc345f8c88bc4c90482803b741e92f9d993f4d0c6a911643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19661301$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1850996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHAHABI, N. A</creatorcontrib><creatorcontrib>BURTNESS, M. Z</creatorcontrib><creatorcontrib>SHARP, B. M</creatorcontrib><title>N-acetyl-β-endorphin1-31 antagonizes the suppressive effect of β-endorphin1-31 on murine splenocyte proliferation via a naloxone-resistant receptor</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>High affinity binding sites for beta-endorphin1-31 (beta-EP) have been observed on transformed mononuclear cells such as the human U937 monocyte-like cell line and the murine EL4-thymoma line, and on normal murine splenocytes. Binding of beta-EP at these sites is resistant to competition by naloxone and other opiate receptor ligands but sensitive to N-acetyl-beta-endorphin1-31 (N-Ac), cations and GTP-gamma-sulfate. Thus, the following studies were done to determine the functional significance of binding beta-EP and N-Ac. beta-EP suppressed phytohemagglutinin (PHA)-stimulated [3H]thymidine uptake in a dose-dependent, naloxone-insensitive fashion. beta-Endorphin1-27, (des)-tyrosine beta-endorphin2-31, or N-Ac failed to duplicate the suppressive effect of beta-EP. However, N-Ac, which is equipotent to beta-EP at displacing 125I-beta-EP bound to murine splenocytes or U937 cells, antagonized the suppressive effect of beta-EP. Taken together with previous binding studies, the present observations suggest that beta-EP effects receptor-mediated responses on normal immunocytes that do not depend on the activation of neuronal-like opiate receptors which are naloxone-sensitive. N-Ac, which shows minimal binding to such brain opiate receptors, is a potent functional antagonist of the naloxone-insensitive immunocyte receptor for beta-EP.</description><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>beta-Endorphin - analogs & derivatives</subject><subject>beta-Endorphin - antagonists & inhibitors</subject><subject>beta-Endorphin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>DNA Replication - drug effects</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Naloxone - pharmacology</subject><subject>Peptide Fragments - antagonists & inhibitors</subject><subject>Peptide Fragments - pharmacology</subject><subject>Phytohemagglutinins</subject><subject>Proteins</subject><subject>Receptors, Opioid - drug effects</subject><subject>Receptors, Opioid - physiology</subject><subject>Spleen</subject><subject>Thymidine - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkU1uFDEQhS0ECkPgBiB5A4KFwdV2O_YSRfxJEWwgYme53WVi1GM3tjtiuAcX4SCciR5mRBasavHe96r0ipCHwJ8DB_WCc65YZ-DzUwPPDKi-Z5e3yAa44awDLm-TzT_LXXKv1q-cA0hlTsgJ6J4bozbk53vmPLbdxH7_YpjGXOarmIAJoC419yWn-AMrbVdI6zLPBWuN10gxBPSN5kD_w3Ki26XEtALzhCn7XUM6lzzFgMW1uOrX0VFHk5vy95yQraGxtnUdLehxbrncJ3eCmyo-OM5T8un1q4_nb9nFhzfvzl9eMC9E15g6U0ZLKf0oJDgY5OCF7IP2Wg9eesOl7jQXw5kENF0wozEiyJF75QyAkuKUPDnkrvd9W7A2u43V4zS5hHmpVvNedhr0apQHoy-51oLBziVuXdlZ4Hb_Dbuv2u6rtgbs32_YyxV7dMxfhi2ON9Ch_lV_fNRd9W4KxSUf643NKAWCg_gDW7-V9w</recordid><startdate>19910329</startdate><enddate>19910329</enddate><creator>SHAHABI, N. A</creator><creator>BURTNESS, M. Z</creator><creator>SHARP, B. M</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910329</creationdate><title>N-acetyl-β-endorphin1-31 antagonizes the suppressive effect of β-endorphin1-31 on murine splenocyte proliferation via a naloxone-resistant receptor</title><author>SHAHABI, N. A ; BURTNESS, M. Z ; SHARP, B. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-67698444cd341a1b4bc345f8c88bc4c90482803b741e92f9d993f4d0c6a911643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>beta-Endorphin - analogs & derivatives</topic><topic>beta-Endorphin - antagonists & inhibitors</topic><topic>beta-Endorphin - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>DNA Replication - drug effects</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Naloxone - pharmacology</topic><topic>Peptide Fragments - antagonists & inhibitors</topic><topic>Peptide Fragments - pharmacology</topic><topic>Phytohemagglutinins</topic><topic>Proteins</topic><topic>Receptors, Opioid - drug effects</topic><topic>Receptors, Opioid - physiology</topic><topic>Spleen</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHAHABI, N. A</creatorcontrib><creatorcontrib>BURTNESS, M. Z</creatorcontrib><creatorcontrib>SHARP, B. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHAHABI, N. A</au><au>BURTNESS, M. Z</au><au>SHARP, B. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-acetyl-β-endorphin1-31 antagonizes the suppressive effect of β-endorphin1-31 on murine splenocyte proliferation via a naloxone-resistant receptor</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1991-03-29</date><risdate>1991</risdate><volume>175</volume><issue>3</issue><spage>936</spage><epage>942</epage><pages>936-942</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>High affinity binding sites for beta-endorphin1-31 (beta-EP) have been observed on transformed mononuclear cells such as the human U937 monocyte-like cell line and the murine EL4-thymoma line, and on normal murine splenocytes. Binding of beta-EP at these sites is resistant to competition by naloxone and other opiate receptor ligands but sensitive to N-acetyl-beta-endorphin1-31 (N-Ac), cations and GTP-gamma-sulfate. Thus, the following studies were done to determine the functional significance of binding beta-EP and N-Ac. beta-EP suppressed phytohemagglutinin (PHA)-stimulated [3H]thymidine uptake in a dose-dependent, naloxone-insensitive fashion. beta-Endorphin1-27, (des)-tyrosine beta-endorphin2-31, or N-Ac failed to duplicate the suppressive effect of beta-EP. However, N-Ac, which is equipotent to beta-EP at displacing 125I-beta-EP bound to murine splenocytes or U937 cells, antagonized the suppressive effect of beta-EP. Taken together with previous binding studies, the present observations suggest that beta-EP effects receptor-mediated responses on normal immunocytes that do not depend on the activation of neuronal-like opiate receptors which are naloxone-sensitive. N-Ac, which shows minimal binding to such brain opiate receptors, is a potent functional antagonist of the naloxone-insensitive immunocyte receptor for beta-EP.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>1850996</pmid><doi>10.1016/0006-291X(91)91655-V</doi><tpages>7</tpages></addata></record> |
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subjects | Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals beta-Endorphin - analogs & derivatives beta-Endorphin - antagonists & inhibitors beta-Endorphin - pharmacology Biological and medical sciences Cells, Cultured DNA Replication - drug effects Female Fundamental and applied biological sciences. Psychology Kinetics Lymphocyte Activation - drug effects Lymphocytes - drug effects Lymphocytes - immunology Mice Mice, Inbred Strains Naloxone - pharmacology Peptide Fragments - antagonists & inhibitors Peptide Fragments - pharmacology Phytohemagglutinins Proteins Receptors, Opioid - drug effects Receptors, Opioid - physiology Spleen Thymidine - metabolism |
title | N-acetyl-β-endorphin1-31 antagonizes the suppressive effect of β-endorphin1-31 on murine splenocyte proliferation via a naloxone-resistant receptor |
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