A novel method for determining the sensitivity of herpes simplex virus to antiviral compounds
A rapid and sensitive assay was developed to analyse the sensitivity of wild type HSV-1 and HSV-2 isolates with respect to a battery of antiviral substances. In the viral sensitivity assay, human embryonic lung fibroblasts are incubated with the virus isolate and different concentrations of the anti...
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| Veröffentlicht in: | Journal of virological methods 1983-01, Vol.6 (3), p.141-149 |
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| container_end_page | 149 |
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| container_issue | 3 |
| container_start_page | 141 |
| container_title | Journal of virological methods |
| container_volume | 6 |
| creator | Wahren, B. Harmenberg, J. Sundqvist, V.-A. Levén, B. Sköldenberg, B. |
| description | A rapid and sensitive assay was developed to analyse the sensitivity of wild type HSV-1 and HSV-2 isolates with respect to a battery of antiviral substances. In the viral sensitivity assay, human embryonic lung fibroblasts are incubated with the virus isolate and different concentrations of the antivirals. After 1–3 days, the cells are disrupted and analysed for HSV type 1 or 2 antigens by an enzyme-linked immunosorbent assay. Antigens corresponding to 17 plaque-forming units were detectable after 1 day of incubation. After 3 days, HSV antigens derived from less than one plaqueforming unit were measurable. The sensitivities of 22 HSV-1 and 19 HSV-2 primary isolates from untreated patients were tested against adenosine arabinoside, acyclovir, phosphonoformic acid and iododeoxyuridine. Each isolate was found to have an individual pattern of sensitivities to the different antivirals. Five isolates were judged to be relatively resistant to one or more of the drugs tested. |
| doi_str_mv | 10.1016/0166-0934(83)90026-5 |
| format | Article |
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In the viral sensitivity assay, human embryonic lung fibroblasts are incubated with the virus isolate and different concentrations of the antivirals. After 1–3 days, the cells are disrupted and analysed for HSV type 1 or 2 antigens by an enzyme-linked immunosorbent assay. Antigens corresponding to 17 plaque-forming units were detectable after 1 day of incubation. After 3 days, HSV antigens derived from less than one plaqueforming unit were measurable. The sensitivities of 22 HSV-1 and 19 HSV-2 primary isolates from untreated patients were tested against adenosine arabinoside, acyclovir, phosphonoformic acid and iododeoxyuridine. Each isolate was found to have an individual pattern of sensitivities to the different antivirals. 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Five isolates were judged to be relatively resistant to one or more of the drugs tested.</description><subject>Antigens, Viral - analysis</subject><subject>Antiviral Agents - pharmacology</subject><subject>antiviral substances</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>herpes simplex virus 1</subject><subject>herpes simplex virus 2</subject><subject>herpes simplex viruses</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Simplexvirus - drug effects</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVoSTZp_0EDOpXm4Ga0sj58CYSQtIVAL8mxCFsaZVVsy5Hkpfn38XaXHNvDMDDzzDvwEPKJwVcGTF4uJStoeP1F84sGYC0rcURWTKtmGev6HVm9ISfkNOffACAU58fkWHKQwGFFfl3TMW6xpwOWTXTUx0QdFkxDGMP4RMsGacYxhxK2obzQ6OkG04SZ5jBMPf6h25DmTEuk7bhjUttTG4cpzqPLH8h73_YZPx76GXm8u324-V7d__z24-b6vrI1U6XywkkJgq9ReStq6GTjlAaPmgm15o1Yd5IrqZgTXjshLQjsWq9V13BnGfAz8nmfO6X4PGMuZgjZYt-3I8Y5G72Ec7GI-B_IuGg0cLaA9R60Keac0JsphaFNL4aB2ek3O7dm59Zobv7qN2I5Oz_kz92A7u3o4HvZX-33uNjYBkwm24CjRRcS2mJcDP9-8Ar4jpRs</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Wahren, B.</creator><creator>Harmenberg, J.</creator><creator>Sundqvist, V.-A.</creator><creator>Levén, B.</creator><creator>Sköldenberg, B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>A novel method for determining the sensitivity of herpes simplex virus to antiviral compounds</title><author>Wahren, B. ; Harmenberg, J. ; Sundqvist, V.-A. ; Levén, B. ; Sköldenberg, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f5d660532e7fc540b69d780fe815723952b637671d5f8d56c05ebaf87b93dc103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Antigens, Viral - analysis</topic><topic>Antiviral Agents - pharmacology</topic><topic>antiviral substances</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>herpes simplex virus 1</topic><topic>herpes simplex virus 2</topic><topic>herpes simplex viruses</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Simplexvirus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wahren, B.</creatorcontrib><creatorcontrib>Harmenberg, J.</creatorcontrib><creatorcontrib>Sundqvist, V.-A.</creatorcontrib><creatorcontrib>Levén, B.</creatorcontrib><creatorcontrib>Sköldenberg, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wahren, B.</au><au>Harmenberg, J.</au><au>Sundqvist, V.-A.</au><au>Levén, B.</au><au>Sköldenberg, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel method for determining the sensitivity of herpes simplex virus to antiviral compounds</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>6</volume><issue>3</issue><spage>141</spage><epage>149</epage><pages>141-149</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><abstract>A rapid and sensitive assay was developed to analyse the sensitivity of wild type HSV-1 and HSV-2 isolates with respect to a battery of antiviral substances. 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| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1983-01, Vol.6 (3), p.141-149 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80533509 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antigens, Viral - analysis Antiviral Agents - pharmacology antiviral substances Enzyme-Linked Immunosorbent Assay herpes simplex virus 1 herpes simplex virus 2 herpes simplex viruses Microbial Sensitivity Tests - methods Simplexvirus - drug effects |
| title | A novel method for determining the sensitivity of herpes simplex virus to antiviral compounds |
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