Skeletal development and formation of osteoclast-like cells from in situ progenitors in fetal mouse metatarsals cultured in chemically defined medium

An in vitro model system is described, using metatarsal explants from 15-day mouse embryos (E15) cultured in serumless chemically defined medium, to study fetal skeletal development with particular emphasis on de novo osteoclast formation. The normal pattern of growth and differentiation observed in...

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Veröffentlicht in:	Bone and mineral 1991-03, Vol.12 (3), p.141-155
Hauptverfasser:	Minkin, Cedric, James, Steven St, Tao, Hong-hui, Yu, Xiao-hui, Pockwinse, Shirwin, MaCkay, Carol, Marks, Sandy C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Calcitriol - pharmacology Cartilage - cytology Cartilage - embryology Cell Differentiation Cells, Cultured Culture Media Development Embryo, Mammalian Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fundamental and applied biological sciences. Psychology Kinetics Metatarsal Bones - embryology Mice Mice, Inbred Strains Microscopy, Electron Mouse Organ culture Organ Culture Techniques Organogenesis. Physiological fonctions Osteoclast Osteoclasts - cytology Osteoclasts - ultrastructure Osteogenesis Stem Cells - cytology Ultrastructure
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