Transmembrane currents in frog olfactory cilia
We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ram...
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| Veröffentlicht in: | The Journal of membrane biology 1991-02, Vol.120 (1), p.75-81 |
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| container_title | The Journal of membrane biology |
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| creator | KLEENE, S. J GESTELAND, R. C |
| description | We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low. |
| doi_str_mv | 10.1007/BF01868593 |
| format | Article |
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J ; GESTELAND, R. C</creator><creatorcontrib>KLEENE, S. J ; GESTELAND, R. C</creatorcontrib><description>We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/BF01868593</identifier><identifier>PMID: 1850487</identifier><identifier>CODEN: JMBBBO</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Action Potentials - physiology ; Animals ; Biological and medical sciences ; Bucladesine - pharmacology ; cilia ; Cilia - physiology ; Cyclic AMP - pharmacology ; Egtazic Acid - analogs & derivatives ; Egtazic Acid - pharmacology ; Electric Conductivity ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; membrane currents ; Membrane Potentials - physiology ; Microelectrodes ; Neurons - physiology ; Odorants ; olfactory apparatus ; olfactory epithelium ; Olfactory Mucosa - innervation ; Olfactory system and olfaction. 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J</creatorcontrib><creatorcontrib>GESTELAND, R. C</creatorcontrib><title>Transmembrane currents in frog olfactory cilia</title><title>The Journal of membrane biology</title><addtitle>J Membr Biol</addtitle><description>We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.</description><subject>Action Potentials - physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bucladesine - pharmacology</subject><subject>cilia</subject><subject>Cilia - physiology</subject><subject>Cyclic AMP - pharmacology</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Egtazic Acid - pharmacology</subject><subject>Electric Conductivity</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>membrane currents</subject><subject>Membrane Potentials - physiology</subject><subject>Microelectrodes</subject><subject>Neurons - physiology</subject><subject>Odorants</subject><subject>olfactory apparatus</subject><subject>olfactory epithelium</subject><subject>Olfactory Mucosa - innervation</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Rana pipiens</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-2631</issn><issn>1432-1424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1LA0EURQdRYow29sI2Wggb33zPlCYYFQI2sV4mkzeyspuNM7tF_r0jCaS0usU9XC6HkFsKUwqgn2YLoEYZafkZGVPBWUkFE-dkDMBYyRSnl-QqpW8AqrUSIzKiRoIwekymq-i2qcV2nRMLP8SI2z4V9bYIsfsquiY433dxX_i6qd01uQiuSXhzzAn5XLys5m_l8uP1ff68LD0XvC-tkpwrJ4VF0OBU2CAaVE4JRCoVGkW11FyB2uRWKWODsBI8ci6pDo5PyMNhdxe7nwFTX7V18tg0-WQ3pMqAZEYD_AtSaaWUlGXw8QD62KUUMVS7WLcu7isK1Z_F6mQxw3fH1WHd4uaEHrTl_v7Yu-RdE7I7X6cTZnUe0pr_Am1Udso</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>KLEENE, S. J</creator><creator>GESTELAND, R. 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Psychology</topic><topic>membrane currents</topic><topic>Membrane Potentials - physiology</topic><topic>Microelectrodes</topic><topic>Neurons - physiology</topic><topic>Odorants</topic><topic>olfactory apparatus</topic><topic>olfactory epithelium</topic><topic>Olfactory Mucosa - innervation</topic><topic>Olfactory system and olfaction. Gustatory system and gustation</topic><topic>Rana pipiens</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KLEENE, S. J</creatorcontrib><creatorcontrib>GESTELAND, R. C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of membrane biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KLEENE, S. J</au><au>GESTELAND, R. C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transmembrane currents in frog olfactory cilia</atitle><jtitle>The Journal of membrane biology</jtitle><addtitle>J Membr Biol</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>120</volume><issue>1</issue><spage>75</spage><epage>81</epage><pages>75-81</pages><issn>0022-2631</issn><eissn>1432-1424</eissn><coden>JMBBBO</coden><abstract>We have measured transmembrane currents in intact single cilia from frog olfactory receptor neurons. A single cilium on a neuron was sucked into a patch pipette, and a high-resistance seal was formed near the base of the cilium. Action potentials could be induced by applying suction or a voltage ramp to the ciliary membrane. A transient current was seen in some cells on stimulation with odorants. After excision from the cell, most of the cilia showed increased conductance in a bath containing cAMP, indicating that the cytoplasmic face of the ciliary membrane was accessible to the bath. The estimated resistance of a single cilium was surprisingly low.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>1850487</pmid><doi>10.1007/BF01868593</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of membrane biology, 1991-02, Vol.120 (1), p.75-81 |
| issn | 0022-2631 1432-1424 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Action Potentials - physiology Animals Biological and medical sciences Bucladesine - pharmacology cilia Cilia - physiology Cyclic AMP - pharmacology Egtazic Acid - analogs & derivatives Egtazic Acid - pharmacology Electric Conductivity Electrophysiology Fundamental and applied biological sciences. Psychology membrane currents Membrane Potentials - physiology Microelectrodes Neurons - physiology Odorants olfactory apparatus olfactory epithelium Olfactory Mucosa - innervation Olfactory system and olfaction. Gustatory system and gustation Rana pipiens Vertebrates: nervous system and sense organs |
| title | Transmembrane currents in frog olfactory cilia |
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