Relationship between 2'-hydroxyls and magnesium binding in the hammerhead RNA domain: a model for ribozyme catalysis

The use of deoxyribonucleotide substitution in RNA (mixed RNA/DNA polymers) permits an evaluation of the role of 2'-hydroxyl groups in ribozyme catalysis. Specific deoxyribonucleotide substitution at G9 and A13 of the ribozyme decreases the catalytic activity (kcat) of the ribozyme by factors o...

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Veröffentlicht in:	Biochemistry (Easton) 1991-04, Vol.30 (16), p.4020-4025
Hauptverfasser:	Perreault, Jean Pierre, Labuda, Damian, Usman, Nassim, Yang, Jing Hua, Cedergren, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Biological and medical sciences Catalysis DNA - chemical synthesis Fundamental and applied biological sciences. Psychology Hydroxylation Kinetics Magnesium - chemistry Mechanisms. Catalysis. Electron transfer. Models Models, Genetic Molecular biophysics Molecular Sequence Data Nucleic Acid Conformation Physical chemistry in biology RNA - chemical synthesis RNA - chemistry RNA, Catalytic - metabolism Thermodynamics
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