Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins
A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA prepara...
Gespeichert in:
| Veröffentlicht in: | Gene 1983-04, Vol.22 (1), p.59-68 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 68 |
|---|---|
| container_issue | 1 |
| container_start_page | 59 |
| container_title | Gene |
| container_volume | 22 |
| creator | Brûlet, Philippe G. Roskam, Willem |
| description | A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA preparation has been used to prepare a hybridization probe to screen a trophoblastoma cDNA library in Escherichia coli. A small proportion of the clones hybridized to the probe, and among these a specific clone was identified. |
| doi_str_mv | 10.1016/0378-1119(83)90064-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80525252</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0378111983900641</els_id><sourcerecordid>80525252</sourcerecordid><originalsourceid>FETCH-LOGICAL-c308t-59e68e7b09588d2dbdacedead1a466375f7496a09bb225ae23c7c805625c51253</originalsourceid><addsrcrecordid>eNp9Uc1uFSEYJY2mXmvfoCasjC5GYbjMMC5MbmqrTZrWGLsmDHzTYgYYgdukfRifVei96VJYkPCdn3znIHRCyUdKaPeJsF40lNLhvWAfBkK6dUMP0IqKfmgIYeIFWj1DXqHXKf0m5XDeHqLDjhHe93SF_l64JYZ7MNhBvgsGTyFiPQdv_S3-erXBOrhlBgc-q_iAc8DO-gJxP6826TP-EWFRUWUbPA4TVvjuYYzW2MfdV5EeAU8xOLxso51s9alMDF4HUz2szxAdGKtyQdpZVatKzGB9eoNeTmpOcLx_j9DN-dmv0-_N5fW3i9PNZaMZEbnhA3QC-pEMXAjTmtEoDQaUoWrddaznU78eOkWGcWxbrqBluteC8K7lmtOWsyP0bqdbjP9sIWXpbNIwz8pD2CZZsG29BbjeAXUMKUWY5BKtK9FISmStRdbMZc1cCiafapG00N7u9bdjWfaZtO-hzL_s5lCWvLcQZdK2ZFRyiaCzNMH-3-Af78yfSA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80525252</pqid></control><display><type>article</type><title>Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Brûlet, Philippe ; G. Roskam, Willem</creator><creatorcontrib>Brûlet, Philippe ; G. Roskam, Willem</creatorcontrib><description>A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA preparation has been used to prepare a hybridization probe to screen a trophoblastoma cDNA library in Escherichia coli. A small proportion of the clones hybridized to the probe, and among these a specific clone was identified.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(83)90064-1</identifier><identifier>PMID: 6305771</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cells, Cultured ; Cloning, Molecular - methods ; DNA, Recombinant ; early embryonic differentiation ; Escherichia coli - genetics ; Escherichia coli host ; Female ; high resolution mRNA purification ; Intermediate Filament Proteins - genetics ; Mice ; mouse trophoblastoma cells ; Neoplasms, Experimental - genetics ; Nucleic Acid Hybridization ; Pregnancy ; preparative gel electrophoresis ; Recombinant DNA ; RNA, Messenger - genetics ; RNA, Neoplasm - genetics ; screening of cDNA clones ; Trophoblastic Neoplasms - genetics ; Uterine Neoplasms - genetics</subject><ispartof>Gene, 1983-04, Vol.22 (1), p.59-68</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c308t-59e68e7b09588d2dbdacedead1a466375f7496a09bb225ae23c7c805625c51253</citedby><cites>FETCH-LOGICAL-c308t-59e68e7b09588d2dbdacedead1a466375f7496a09bb225ae23c7c805625c51253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(83)90064-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6305771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brûlet, Philippe</creatorcontrib><creatorcontrib>G. Roskam, Willem</creatorcontrib><title>Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins</title><title>Gene</title><addtitle>Gene</addtitle><description>A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA preparation has been used to prepare a hybridization probe to screen a trophoblastoma cDNA library in Escherichia coli. A small proportion of the clones hybridized to the probe, and among these a specific clone was identified.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular - methods</subject><subject>DNA, Recombinant</subject><subject>early embryonic differentiation</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli host</subject><subject>Female</subject><subject>high resolution mRNA purification</subject><subject>Intermediate Filament Proteins - genetics</subject><subject>Mice</subject><subject>mouse trophoblastoma cells</subject><subject>Neoplasms, Experimental - genetics</subject><subject>Nucleic Acid Hybridization</subject><subject>Pregnancy</subject><subject>preparative gel electrophoresis</subject><subject>Recombinant DNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Neoplasm - genetics</subject><subject>screening of cDNA clones</subject><subject>Trophoblastic Neoplasms - genetics</subject><subject>Uterine Neoplasms - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc1uFSEYJY2mXmvfoCasjC5GYbjMMC5MbmqrTZrWGLsmDHzTYgYYgdukfRifVei96VJYkPCdn3znIHRCyUdKaPeJsF40lNLhvWAfBkK6dUMP0IqKfmgIYeIFWj1DXqHXKf0m5XDeHqLDjhHe93SF_l64JYZ7MNhBvgsGTyFiPQdv_S3-erXBOrhlBgc-q_iAc8DO-gJxP6826TP-EWFRUWUbPA4TVvjuYYzW2MfdV5EeAU8xOLxso51s9alMDF4HUz2szxAdGKtyQdpZVatKzGB9eoNeTmpOcLx_j9DN-dmv0-_N5fW3i9PNZaMZEbnhA3QC-pEMXAjTmtEoDQaUoWrddaznU78eOkWGcWxbrqBluteC8K7lmtOWsyP0bqdbjP9sIWXpbNIwz8pD2CZZsG29BbjeAXUMKUWY5BKtK9FISmStRdbMZc1cCiafapG00N7u9bdjWfaZtO-hzL_s5lCWvLcQZdK2ZFRyiaCzNMH-3-Af78yfSA</recordid><startdate>198304</startdate><enddate>198304</enddate><creator>Brûlet, Philippe</creator><creator>G. Roskam, Willem</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198304</creationdate><title>Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins</title><author>Brûlet, Philippe ; G. Roskam, Willem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-59e68e7b09588d2dbdacedead1a466375f7496a09bb225ae23c7c805625c51253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular - methods</topic><topic>DNA, Recombinant</topic><topic>early embryonic differentiation</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli host</topic><topic>Female</topic><topic>high resolution mRNA purification</topic><topic>Intermediate Filament Proteins - genetics</topic><topic>Mice</topic><topic>mouse trophoblastoma cells</topic><topic>Neoplasms, Experimental - genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Pregnancy</topic><topic>preparative gel electrophoresis</topic><topic>Recombinant DNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Neoplasm - genetics</topic><topic>screening of cDNA clones</topic><topic>Trophoblastic Neoplasms - genetics</topic><topic>Uterine Neoplasms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brûlet, Philippe</creatorcontrib><creatorcontrib>G. Roskam, Willem</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brûlet, Philippe</au><au>G. Roskam, Willem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1983-04</date><risdate>1983</risdate><volume>22</volume><issue>1</issue><spage>59</spage><epage>68</epage><pages>59-68</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A procedure for the rapid fractionation of mRNA has been used to enrich mRNAs encoding a set of intermediate filament proteins in trophoblastoma cells. The procedure involves sucrose-gradient fractionation followed by high-resolution preparative gel electrophoresis. Part of the enriched mRNA preparation has been used to prepare a hybridization probe to screen a trophoblastoma cDNA library in Escherichia coli. A small proportion of the clones hybridized to the probe, and among these a specific clone was identified.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6305771</pmid><doi>10.1016/0378-1119(83)90064-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1983-04, Vol.22 (1), p.59-68 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80525252 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cells, Cultured Cloning, Molecular - methods DNA, Recombinant early embryonic differentiation Escherichia coli - genetics Escherichia coli host Female high resolution mRNA purification Intermediate Filament Proteins - genetics Mice mouse trophoblastoma cells Neoplasms, Experimental - genetics Nucleic Acid Hybridization Pregnancy preparative gel electrophoresis Recombinant DNA RNA, Messenger - genetics RNA, Neoplasm - genetics screening of cDNA clones Trophoblastic Neoplasms - genetics Uterine Neoplasms - genetics |
| title | Improved method for cloning DNA complementary to minor mRNAs: Preparation of a hybridization probe from purified mRNAs encoding intermediate filament proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T01%3A34%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20method%20for%20cloning%20DNA%20complementary%20to%20minor%20mRNAs:%20Preparation%20of%20a%20hybridization%20probe%20from%20purified%20mRNAs%20encoding%20intermediate%20filament%20proteins&rft.jtitle=Gene&rft.au=Br%C3%BBlet,%20Philippe&rft.date=1983-04&rft.volume=22&rft.issue=1&rft.spage=59&rft.epage=68&rft.pages=59-68&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/0378-1119(83)90064-1&rft_dat=%3Cproquest_cross%3E80525252%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80525252&rft_id=info:pmid/6305771&rft_els_id=0378111983900641&rfr_iscdi=true |