Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide
The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in oth...
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| Veröffentlicht in: | Analytical biochemistry 1983-02, Vol.129 (1), p.22-30 |
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| container_title | Analytical biochemistry |
| container_volume | 129 |
| creator | Newton, Clive R. Greene, April R. Heathcliffe, Gillian R. Atkinson, Thomas C. Holland, David Markham, Alexander F. Edge, Michael D. |
| description | The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues. |
| doi_str_mv | 10.1016/0003-2697(83)90046-5 |
| format | Article |
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The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(83)90046-5</identifier><identifier>PMID: 6859525</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; formamide ; Formamides ; high-performance liquid chromatography ; ion-exchange ; Oligodeoxyribonucleotides - analysis ; Oligonucleotides - analysis ; sequence effects ; solid-phase ; synthetic oligonucleotide</subject><ispartof>Analytical biochemistry, 1983-02, Vol.129 (1), p.22-30</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-9c1ccda8d04312458b99c5f2a2c95842cbd6302dbddd5cb030c4370f3cf4f9653</citedby><cites>FETCH-LOGICAL-c423t-9c1ccda8d04312458b99c5f2a2c95842cbd6302dbddd5cb030c4370f3cf4f9653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269783900465$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6859525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Newton, Clive R.</creatorcontrib><creatorcontrib>Greene, April R.</creatorcontrib><creatorcontrib>Heathcliffe, Gillian R.</creatorcontrib><creatorcontrib>Atkinson, Thomas C.</creatorcontrib><creatorcontrib>Holland, David</creatorcontrib><creatorcontrib>Markham, Alexander F.</creatorcontrib><creatorcontrib>Edge, Michael D.</creatorcontrib><title>Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. 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The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6859525</pmid><doi>10.1016/0003-2697(83)90046-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1983-02, Vol.129 (1), p.22-30 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Chromatography, High Pressure Liquid Chromatography, Ion Exchange formamide Formamides high-performance liquid chromatography ion-exchange Oligodeoxyribonucleotides - analysis Oligonucleotides - analysis sequence effects solid-phase synthetic oligonucleotide |
| title | Ion-exchange high-performance liquid chromatography of oligodeoxyribonucleotides using formamide |
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