An improved method for isolation of the C-terminal fragment of proteins

An efficient and easily realizable method for the isolation of the C-terminal fragment is described. Proteins are esterified by methanolic HCl and subsequently digested with pepsin. The peptide mixture is submitted to paper electrophoresis in pH 2.1 buffer. The identification of the C-terminal pepti...

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Veröffentlicht in:	Analytical biochemistry 1983-02, Vol.129 (1), p.14-21
Hauptverfasser:	Furka, A, Dibo, G, Kovacs, J, Sebestyen, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chemical Phenomena Chemistry electrophoresis Electrophoresis, Paper Peptide Fragments - isolation & purification peptide mapping Proteins - analysis
Online-Zugang:	Volltext
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