Acylated simian virus 40-specific proteins in the plasma membrane of HeLa cells infected with adenovirus 2-simian virus 40 hybrid virus Ad2+ND2

HeLa cells infected with the adenovirus 2-simian virus 40 (Ad2+SV40) hybrid virus Ad2+ND2 were labeled with either [35S]methionine or [3H]palmitate and fractionated into cytoplasmic, nuclear, and plasma membrane fractions. Analysis of these fractions by sodium dodecyl sulfate-polyacrylamide gel elec...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1983-04, Vol.126 (2), p.717-720
Hauptverfasser:	Klockmann, U, Deppert, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics Adenoviruses, Simian - genetics Cell Transformation, Viral HeLa Cells - metabolism Humans Methionine - metabolism Mutation Palmitic Acid Palmitic Acids - metabolism Subcellular Fractions - metabolism Sulfur Radioisotopes Tritium Viral Proteins - genetics
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Zusammenfassung:	HeLa cells infected with the adenovirus 2-simian virus 40 (Ad2+SV40) hybrid virus Ad2+ND2 were labeled with either [35S]methionine or [3H]palmitate and fractionated into cytoplasmic, nuclear, and plasma membrane fractions. Analysis of these fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the SV40-specific proteins in the plasma membrane fraction were specificially acylated.
ISSN:	0042-6822