Production of 5-methyluridine by immobilized thermostable purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus JTS 859

5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column r...

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Veröffentlicht in:	Journal of biotechnology 1991-02, Vol.17 (2), p.121-131
Hauptverfasser:	Hori, Nobuaki, Watanabe, Mutsumi, Sunagawa, Kenji, Uehara, Kyoko, Mikami, Yoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Methyluridine Bacillus stearothermophilus Bacterial Proteins - metabolism Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Enzymes, Immobilized - metabolism Fundamental and applied biological sciences. Psychology Geobacillus stearothermophilus - enzymology Half-Life Hydrogen-Ion Concentration immobilized enzymes Inosine - metabolism Methods. Procedures. Technologies Pentosyltransferases - metabolism purine-nucleoside phosphorylase Purine-Nucleoside Phosphorylase - metabolism Pyrimidine Phosphorylases pyrimidine-nucleoside phosphorylase Temperature Thermostable purine nucleoside phosphorylase Thermostable pyrimidine nucleoside phosphorylase Thymine - metabolism Uridine - analogs & derivatives Uridine - biosynthesis
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