Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome
Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonst...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-04, Vol.88 (8), p.3253-3257 |
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| creator | Geigenmuller-Gnirke, Ute Weiss, Barbara Wright, Rebecca Schlesinger, Sondra |
| description | Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. \& Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. These different viruses show sequence similarities in their replicative proteins and are thought to have evolved from a common ancestor. |
| doi_str_mv | 10.1073/pnas.88.8.3253 |
| format | Article |
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We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. \& Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. These different viruses show sequence similarities in their replicative proteins and are thought to have evolved from a common ancestor.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.8.3253</identifier><identifier>PMID: 2014249</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Chick Embryo ; Complementary DNA ; Embryos ; Fibroblasts ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Viral ; Genetic Complementation Test ; Genetic Engineering ; Genetics ; Genomes ; Genomics ; Microbiology ; Morphogenesis ; Nucleic Acid Hybridization ; Recombination, Genetic ; RNA ; RNA, Messenger - genetics ; RNA, Viral - genetics ; Sindbis Virus ; Sindbis Virus - genetics ; Transcription, Genetic ; Transfection ; Viral RNA ; Viral Structural Proteins - genetics ; Virology ; Virus Replication ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-04, Vol.88 (8), p.3253-3257</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-7b4db081e1f3ba4e9278bb8619a2761489a18495eafe137d7fc7e486ffeefb9c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2356715$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2356715$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19808394$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2014249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geigenmuller-Gnirke, Ute</creatorcontrib><creatorcontrib>Weiss, Barbara</creatorcontrib><creatorcontrib>Wright, Rebecca</creatorcontrib><creatorcontrib>Schlesinger, Sondra</creatorcontrib><title>Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. \& Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. These different viruses show sequence similarities in their replicative proteins and are thought to have evolved from a common ancestor.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Complementary DNA</subject><subject>Embryos</subject><subject>Fibroblasts</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>Genetic Complementation Test</subject><subject>Genetic Engineering</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Microbiology</subject><subject>Morphogenesis</subject><subject>Nucleic Acid Hybridization</subject><subject>Recombination, Genetic</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Sindbis Virus</subject><subject>Sindbis Virus - genetics</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>Viral RNA</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><subject>Virus Replication</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAUtBCobFuunEDyhd4S7MRJbIlLuyqlUgWofFyN4zxTV4kTbKeFf4-jXbaLhMTJ0sy8eW88CD2nJKekKV9PToWc85znZVGVj9CKEkGzmgnyGK0IKZqMs4I9RYch3BJCRMXJATooCE2oWKFv63GYehjARRXt6PAZxHsAhz9Z17U24K_Wqx5fvz8N-KMfu1lDwJfOgE7qOWHKR6v7BN7beIMVPrPTAkXAF-DGAY7RE6P6AM-27xH68vb88_pddvXh4nJ9epXpivKYNS3rWsIpUFO2ioEoGt62vKZCFU1NGReKciYqUAZo2XSN0Q0wXhsDYFqhyyP0ZuM7ze0AnU6B0uFy8nZQ_pcclZV_M87eyO_jnayWn0jjJ9txP_6YIUQ52KCh75WDlFNyUpFaFPS_QlpTKhhZHPONUPsxBA9mdwslcqlOLtVJziWXS3Vp4OV-gp1821XiX215FbTqjVdO2_DgKjjhpdiPsvj_oXd7pJn7PsLPuLfwn8LEv9jwtyGO_uGesqobWpW_AZ6SxSo</recordid><startdate>19910415</startdate><enddate>19910415</enddate><creator>Geigenmuller-Gnirke, Ute</creator><creator>Weiss, Barbara</creator><creator>Wright, Rebecca</creator><creator>Schlesinger, Sondra</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910415</creationdate><title>Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome</title><author>Geigenmuller-Gnirke, Ute ; Weiss, Barbara ; Wright, Rebecca ; Schlesinger, Sondra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-7b4db081e1f3ba4e9278bb8619a2761489a18495eafe137d7fc7e486ffeefb9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Complementary DNA</topic><topic>Embryos</topic><topic>Fibroblasts</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Viral</topic><topic>Genetic Complementation Test</topic><topic>Genetic Engineering</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Microbiology</topic><topic>Morphogenesis</topic><topic>Nucleic Acid Hybridization</topic><topic>Recombination, Genetic</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Sindbis Virus</topic><topic>Sindbis Virus - genetics</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>Viral RNA</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><topic>Virus Replication</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geigenmuller-Gnirke, Ute</creatorcontrib><creatorcontrib>Weiss, Barbara</creatorcontrib><creatorcontrib>Wright, Rebecca</creatorcontrib><creatorcontrib>Schlesinger, Sondra</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geigenmuller-Gnirke, Ute</au><au>Weiss, Barbara</au><au>Wright, Rebecca</au><au>Schlesinger, Sondra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-04-15</date><risdate>1991</risdate><volume>88</volume><issue>8</issue><spage>3253</spage><epage>3257</epage><pages>3253-3257</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. \& Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. 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| subjects | Animals Biological and medical sciences Cells, Cultured Chick Embryo Complementary DNA Embryos Fibroblasts Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genes Genes, Viral Genetic Complementation Test Genetic Engineering Genetics Genomes Genomics Microbiology Morphogenesis Nucleic Acid Hybridization Recombination, Genetic RNA RNA, Messenger - genetics RNA, Viral - genetics Sindbis Virus Sindbis Virus - genetics Transcription, Genetic Transfection Viral RNA Viral Structural Proteins - genetics Virology Virus Replication Viruses |
| title | Complementation Between Sindbis Viral RNAs Produces Infectious Particles with a Bipartite Genome |
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