Evidence of leukotriene B4 biosynthesis in epithelial lens cells
Metabolism of sodium (14C)-arachidonate by bovine epithelial lens cells was studied in culture. The cells converted arachidonic acid into a major product whose formation was not inhibited by aspirin, a cyclo-oxygenase inhibitor, but was suppressed by nordihydroguaiaretic acid, a lipoxygenase inhibit...
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| Veröffentlicht in: | Prostaglandins leukotrienes and medicine 1983-04, Vol.10 (4), p.381-387 |
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| container_title | Prostaglandins leukotrienes and medicine |
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| creator | Lonchampt, M O Bonne, C Regnault, F Massé, J P Coquelet, C Sincholle, D |
| description | Metabolism of sodium (14C)-arachidonate by bovine epithelial lens cells was studied in culture. The cells converted arachidonic acid into a major product whose formation was not inhibited by aspirin, a cyclo-oxygenase inhibitor, but was suppressed by nordihydroguaiaretic acid, a lipoxygenase inhibitor and by dexamethasone. This metabolite co-migrated with leukotriene B4 in thin layer chromatography and high pressure liquid chromatography. These data represent the first evidence for a lipoxygenase product in the lens. LTB4 could play an important role in the physiopathology of this organ. |
| doi_str_mv | 10.1016/0262-1746(83)90050-1 |
| format | Article |
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The cells converted arachidonic acid into a major product whose formation was not inhibited by aspirin, a cyclo-oxygenase inhibitor, but was suppressed by nordihydroguaiaretic acid, a lipoxygenase inhibitor and by dexamethasone. This metabolite co-migrated with leukotriene B4 in thin layer chromatography and high pressure liquid chromatography. These data represent the first evidence for a lipoxygenase product in the lens. 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| ispartof | Prostaglandins leukotrienes and medicine, 1983-04, Vol.10 (4), p.381-387 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Arachidonate Lipoxygenases Arachidonic Acids - metabolism Aspirin - pharmacology Catechols - pharmacology Cattle Cells, Cultured Cyclooxygenase Inhibitors Epithelium - metabolism Lens Capsule, Crystalline - metabolism Lens, Crystalline - metabolism Leukotriene B4 - biosynthesis Lipoxygenase Inhibitors Masoprocol |
| title | Evidence of leukotriene B4 biosynthesis in epithelial lens cells |
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