Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene
We have determined the nucleotide (nt) sequence of the wild-type div + gene of Bacillus subtilis which complements the temperature-sensitive div-341 mutation and is involved in cell septation, sporulation, secretion of extracellular enzymes, development of competence, autolysis and spore outgrowth....
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| Veröffentlicht in: | Gene 1991-02, Vol.98 (1), p.101-105 |
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| container_title | Gene |
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| creator | Yoshito, Sadaie Hiromu, Takamatsu Kouji, Nakamura Kunio, Yamane |
| description | We have determined the nucleotide (nt) sequence of the wild-type
div
+ gene of
Bacillus subtilis which complements the temperature-sensitive
div-341 mutation and is involved in cell septation, sporulation, secretion of extracellular enzymes, development of competence, autolysis and spore outgrowth. It has an open reading frame encoding 841 amino acids (aa) with homology to the
Escherichia coli secA gene, which is involved in protein secretion and cell separation. The deduced aa sequence of the
B. subtilis div
+ gene shares 50% identity with that of the
E. coli secA gene, and highly homologous regions were observed in the N-terminal portions. DNA-DNA hybridization with the
E. coli secA gene as the probe showed that the
div
+ gene could be easily detected by homology and that a single copy of the homologous gene was present in
B. subtilis. Since both genes are similar in their functions and deduced aa sequences, we propose that the
div
+ gene is the counterpart of the
secA gene of
E. coli. |
| doi_str_mv | 10.1016/0378-1119(91)90110-W |
| format | Article |
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div
+ gene of
Bacillus subtilis which complements the temperature-sensitive
div-341 mutation and is involved in cell septation, sporulation, secretion of extracellular enzymes, development of competence, autolysis and spore outgrowth. It has an open reading frame encoding 841 amino acids (aa) with homology to the
Escherichia coli secA gene, which is involved in protein secretion and cell separation. The deduced aa sequence of the
B. subtilis div
+ gene shares 50% identity with that of the
E. coli secA gene, and highly homologous regions were observed in the N-terminal portions. DNA-DNA hybridization with the
E. coli secA gene as the probe showed that the
div
+ gene could be easily detected by homology and that a single copy of the homologous gene was present in
B. subtilis. Since both genes are similar in their functions and deduced aa sequences, we propose that the
div
+ gene is the counterpart of the
secA gene of
E. coli.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(91)90110-W</identifier><identifier>PMID: 1901557</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacillus subtilis - genetics ; Bacillus subtilis - physiology ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; cell division ; Cloning, Molecular ; competence ; DNA Probes ; DNA, Bacterial ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genes. Genome ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; Nucleic Acid Hybridization ; Open Reading Frames ; Recombinant DNA ; Restriction Mapping ; secretion ; Sequence Homology, Nucleic Acid ; sporulation ; Temperature</subject><ispartof>Gene, 1991-02, Vol.98 (1), p.101-105</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-d541cb6402de55e1673fe3f770a73aeb9a834fa9d0e60dd211c3b676443eb3d63</citedby><cites>FETCH-LOGICAL-c453t-d541cb6402de55e1673fe3f770a73aeb9a834fa9d0e60dd211c3b676443eb3d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/037811199190110W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19514132$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1901557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshito, Sadaie</creatorcontrib><creatorcontrib>Hiromu, Takamatsu</creatorcontrib><creatorcontrib>Kouji, Nakamura</creatorcontrib><creatorcontrib>Kunio, Yamane</creatorcontrib><title>Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene</title><title>Gene</title><addtitle>Gene</addtitle><description>We have determined the nucleotide (nt) sequence of the wild-type
div
+ gene of
Bacillus subtilis which complements the temperature-sensitive
div-341 mutation and is involved in cell septation, sporulation, secretion of extracellular enzymes, development of competence, autolysis and spore outgrowth. It has an open reading frame encoding 841 amino acids (aa) with homology to the
Escherichia coli secA gene, which is involved in protein secretion and cell separation. The deduced aa sequence of the
B. subtilis div
+ gene shares 50% identity with that of the
E. coli secA gene, and highly homologous regions were observed in the N-terminal portions. DNA-DNA hybridization with the
E. coli secA gene as the probe showed that the
div
+ gene could be easily detected by homology and that a single copy of the homologous gene was present in
B. subtilis. Since both genes are similar in their functions and deduced aa sequences, we propose that the
div
+ gene is the counterpart of the
secA gene of
E. coli.</description><subject>Amino Acid Sequence</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - physiology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>cell division</subject><subject>Cloning, Molecular</subject><subject>competence</subject><subject>DNA Probes</subject><subject>DNA, Bacterial</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genes. Genome</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleic Acid Hybridization</subject><subject>Open Reading Frames</subject><subject>Recombinant DNA</subject><subject>Restriction Mapping</subject><subject>secretion</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>sporulation</subject><subject>Temperature</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1vFCEUhkmjqdvaf1ATbjRtzChngPm4MalNqyZNvNCml4SBM91j2J0VZtbsvy_7EeuV3HDxPu8BHhg7B_EBBFQfhaybAgDaixYuWwEgiocjNoOmbgshZPOCzf4ir9hJSr9EXlqXx-wYMq91PWPDD_w94dLR8pFHXKMNiSdaULCRxg0fej7Okf-h4Itxs0Luaf2eP-ISt9Fn6yiEKTembqRAiY_Djr9Jbo6R3Jwsd0MgntBd7Wqv2cs-n4Fnh_2U3d_e_Lz-Wtx9__Lt-uqucErLsfBagesqJUqPWiNUtexR9nUtbC0tdq1tpOpt6wVWwvsSwMmuqiulJHbSV_KUvdvPXcUhPzCNZkHJYQh2icOUTCO00KpsMqj2oItDShF7s4q0sHFjQJitZ7OVaLYSTQtm59k85Nqbw_ypW6B_Lu3F5vztIbfJ2dBHmx2nfzANCmSZuU97DrOMNWE0yVH-D_QU0Y3GD_T_izwBVjyaCQ</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>Yoshito, Sadaie</creator><creator>Hiromu, Takamatsu</creator><creator>Kouji, Nakamura</creator><creator>Kunio, Yamane</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910201</creationdate><title>Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene</title><author>Yoshito, Sadaie ; Hiromu, Takamatsu ; Kouji, Nakamura ; Kunio, Yamane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-d541cb6402de55e1673fe3f770a73aeb9a834fa9d0e60dd211c3b676443eb3d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacillus subtilis - physiology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>cell division</topic><topic>Cloning, Molecular</topic><topic>competence</topic><topic>DNA Probes</topic><topic>DNA, Bacterial</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genes. Genome</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleic Acid Hybridization</topic><topic>Open Reading Frames</topic><topic>Recombinant DNA</topic><topic>Restriction Mapping</topic><topic>secretion</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>sporulation</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshito, Sadaie</creatorcontrib><creatorcontrib>Hiromu, Takamatsu</creatorcontrib><creatorcontrib>Kouji, Nakamura</creatorcontrib><creatorcontrib>Kunio, Yamane</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshito, Sadaie</au><au>Hiromu, Takamatsu</au><au>Kouji, Nakamura</au><au>Kunio, Yamane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>98</volume><issue>1</issue><spage>101</spage><epage>105</epage><pages>101-105</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>We have determined the nucleotide (nt) sequence of the wild-type
div
+ gene of
Bacillus subtilis which complements the temperature-sensitive
div-341 mutation and is involved in cell septation, sporulation, secretion of extracellular enzymes, development of competence, autolysis and spore outgrowth. It has an open reading frame encoding 841 amino acids (aa) with homology to the
Escherichia coli secA gene, which is involved in protein secretion and cell separation. The deduced aa sequence of the
B. subtilis div
+ gene shares 50% identity with that of the
E. coli secA gene, and highly homologous regions were observed in the N-terminal portions. DNA-DNA hybridization with the
E. coli secA gene as the probe showed that the
div
+ gene could be easily detected by homology and that a single copy of the homologous gene was present in
B. subtilis. Since both genes are similar in their functions and deduced aa sequences, we propose that the
div
+ gene is the counterpart of the
secA gene of
E. coli.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1901557</pmid><doi>10.1016/0378-1119(91)90110-W</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gene, 1991-02, Vol.98 (1), p.101-105 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80505428 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acid Sequence Bacillus subtilis - genetics Bacillus subtilis - physiology Base Sequence Biological and medical sciences Blotting, Southern cell division Cloning, Molecular competence DNA Probes DNA, Bacterial Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes. Genome Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Nucleic Acid Hybridization Open Reading Frames Recombinant DNA Restriction Mapping secretion Sequence Homology, Nucleic Acid sporulation Temperature |
| title | Sequencing reveals similarity of the wild-type div+ gene of Bacillus subtilis to the Escherichia coli secA gene |
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