Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study
The development of DNA and RNA synthesis in the germ cell population was studied after a 3H‐thymidine or 3H‐uridine pulse at each stage of spermatogenesis. The autoradiographic results show that the first sign (after 3 days in vitro) of cellular changes is an increase in RNA synthesis which reaches...
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| Veröffentlicht in: | The Journal of experimental zoology 1983-04, Vol.226 (1), p.143-149 |
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| creator | Bertout, Marc |
| description | The development of DNA and RNA synthesis in the germ cell population was studied after a 3H‐thymidine or 3H‐uridine pulse at each stage of spermatogenesis. The autoradiographic results show that the first sign (after 3 days in vitro) of cellular changes is an increase in RNA synthesis which reaches a maximum at day 5. DNA replication (premeiotic S phase) occurred at day 7, then cells entered meiotic prophase (day 9). Meiotic divisions and spermiogenesis occurred after 11 days. Silver grain counts permit the conclusion that RNA synthesis is clearly higher during premeiotic interphase (days 3–7) than during spermatogonial proliferation (day 0). It appears therefore that male meiotic differentiation in Nereidae is accompanied by increased RNA synthesis. |
| doi_str_mv | 10.1002/jez.1402260117 |
| format | Article |
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The autoradiographic results show that the first sign (after 3 days in vitro) of cellular changes is an increase in RNA synthesis which reaches a maximum at day 5. DNA replication (premeiotic S phase) occurred at day 7, then cells entered meiotic prophase (day 9). Meiotic divisions and spermiogenesis occurred after 11 days. Silver grain counts permit the conclusion that RNA synthesis is clearly higher during premeiotic interphase (days 3–7) than during spermatogonial proliferation (day 0). It appears therefore that male meiotic differentiation in Nereidae is accompanied by increased RNA synthesis.</description><identifier>ISSN: 0022-104X</identifier><identifier>EISSN: 1097-010X</identifier><identifier>DOI: 10.1002/jez.1402260117</identifier><identifier>PMID: 6189961</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Annelida - physiology ; Autoradiography ; DNA - biosynthesis ; Male ; Nereis diversicolor ; RNA - biosynthesis ; Spermatogenesis</subject><ispartof>The Journal of experimental zoology, 1983-04, Vol.226 (1), p.143-149</ispartof><rights>Copyright © 1983 Wiley‐Liss, Inc., A Wiley Company</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4097-6e9f2c3253f6c172cbc86b6ee8142c40541927188041dfe85b7f3e7ee47dc37c3</citedby><cites>FETCH-LOGICAL-c4097-6e9f2c3253f6c172cbc86b6ee8142c40541927188041dfe85b7f3e7ee47dc37c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjez.1402260117$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjez.1402260117$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6189961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertout, Marc</creatorcontrib><title>Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study</title><title>The Journal of experimental zoology</title><addtitle>J. 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It appears therefore that male meiotic differentiation in Nereidae is accompanied by increased RNA synthesis.</description><subject>Animals</subject><subject>Annelida - physiology</subject><subject>Autoradiography</subject><subject>DNA - biosynthesis</subject><subject>Male</subject><subject>Nereis diversicolor</subject><subject>RNA - biosynthesis</subject><subject>Spermatogenesis</subject><issn>0022-104X</issn><issn>1097-010X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EKqFw5YbkEyqHDf7YXe9yC21pC1FAAkTFxXLs2cRlY6e2F1ju_b9xlaiIU08z0vu9Nxo9hJ5TMqWEsNdX8GdKS8JYTSgVD9CEklYUhJLLh2iSAVZQUl4-Rk9ivCIZqYg4QAc1bdq2phN083l0aQ0RIvYddoPuwWqstDURmyFYt8JxC2Gjkl-Bg2gjtg4vIEDejP0JIVrtex_w0cw56K1R-JPvR71WkNSrN3iGrwflkk0qZRqrIfmgjPWroLbrfCqmwYxP0aNO9RGe7ech-vru9MvxeTH_eHZxPJsXurz9qoa2Y5qzine1poLppW7qZQ3Q0JJlpCppywRtGlJS00FTLUXHQQCUwmguND9EL3e52-CvB4hJbmzU0PfKgR-izMa2olzcC2aGCkJ4Bqc7UAcfY4BOboPdqDBKSuRtQTIXJP8VlA0v9snDcgPmDt83kvV2p_-yPYz3pMn3p9__yy52XhsT_L7zqvBD1oKLSn5bnMkP1eJcnCzeyjn_C4h7rjM</recordid><startdate>198304</startdate><enddate>198304</enddate><creator>Bertout, Marc</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198304</creationdate><title>Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study</title><author>Bertout, Marc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4097-6e9f2c3253f6c172cbc86b6ee8142c40541927188041dfe85b7f3e7ee47dc37c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Annelida - physiology</topic><topic>Autoradiography</topic><topic>DNA - biosynthesis</topic><topic>Male</topic><topic>Nereis diversicolor</topic><topic>RNA - biosynthesis</topic><topic>Spermatogenesis</topic><toplevel>online_resources</toplevel><creatorcontrib>Bertout, Marc</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of experimental zoology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertout, Marc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study</atitle><jtitle>The Journal of experimental zoology</jtitle><addtitle>J. Exp. Zool</addtitle><date>1983-04</date><risdate>1983</risdate><volume>226</volume><issue>1</issue><spage>143</spage><epage>149</epage><pages>143-149</pages><issn>0022-104X</issn><eissn>1097-010X</eissn><abstract>The development of DNA and RNA synthesis in the germ cell population was studied after a 3H‐thymidine or 3H‐uridine pulse at each stage of spermatogenesis. The autoradiographic results show that the first sign (after 3 days in vitro) of cellular changes is an increase in RNA synthesis which reaches a maximum at day 5. DNA replication (premeiotic S phase) occurred at day 7, then cells entered meiotic prophase (day 9). Meiotic divisions and spermiogenesis occurred after 11 days. Silver grain counts permit the conclusion that RNA synthesis is clearly higher during premeiotic interphase (days 3–7) than during spermatogonial proliferation (day 0). It appears therefore that male meiotic differentiation in Nereidae is accompanied by increased RNA synthesis.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>6189961</pmid><doi>10.1002/jez.1402260117</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Journal of experimental zoology, 1983-04, Vol.226 (1), p.143-149 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Annelida - physiology Autoradiography DNA - biosynthesis Male Nereis diversicolor RNA - biosynthesis Spermatogenesis |
| title | Syntheses of nucleic acids during spermatogenesis in Nereis diversicolor (Annelida Polychaeta): A quantitative autoradiographic study |
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