A New Solid-Phase Enzyme-Linked Immunosorbent Assay for Specific Antibodies to Measles Virus
A convenient and flexible enzyme-linked immunosorbent assay system for the detection of specific antibodies to measles virus has been developed. In this system infected cells are desiccated on 96-well microtiter plates and stored at room temperature. After incubation of samples to be tested in the c...
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| Veröffentlicht in: | The Journal of infectious diseases 1983-06, Vol.147 (6), p.1055-1059 |
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| container_issue | 6 |
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| container_title | The Journal of infectious diseases |
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| creator | Rice, George P. A. Casali, Paolo Oldstone, Michael B. A. |
| description | A convenient and flexible enzyme-linked immunosorbent assay system for the detection of specific antibodies to measles virus has been developed. In this system infected cells are desiccated on 96-well microtiter plates and stored at room temperature. After incubation of samples to be tested in the cell-coated plates and subsequent washing, bound antibodies are detected with a peroxidase-conjugated staphylococcal protein A probe. After another washing and the addition of the appropriate substrate, the amount of bound probe is estimated by colorimetric analysis. This technique offers several advantages. The need for a purified viral antigen source is obviated. The plates are easily prepared and can be stored for months at room temperature. Major viral epitopes, including surface glycoproteins as well as cytoplasmic viral antigens, are preserved despite desiccation. The method is more sensitive than the conventional means of virus-specific antibody detection. |
| doi_str_mv | 10.1093/infdis/147.6.1055 |
| format | Article |
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A.</creatorcontrib><creatorcontrib>Casali, Paolo</creatorcontrib><creatorcontrib>Oldstone, Michael B. A.</creatorcontrib><title>A New Solid-Phase Enzyme-Linked Immunosorbent Assay for Specific Antibodies to Measles Virus</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>A convenient and flexible enzyme-linked immunosorbent assay system for the detection of specific antibodies to measles virus has been developed. In this system infected cells are desiccated on 96-well microtiter plates and stored at room temperature. After incubation of samples to be tested in the cell-coated plates and subsequent washing, bound antibodies are detected with a peroxidase-conjugated staphylococcal protein A probe. After another washing and the addition of the appropriate substrate, the amount of bound probe is estimated by colorimetric analysis. This technique offers several advantages. The need for a purified viral antigen source is obviated. The plates are easily prepared and can be stored for months at room temperature. Major viral epitopes, including surface glycoproteins as well as cytoplasmic viral antigens, are preserved despite desiccation. The method is more sensitive than the conventional means of virus-specific antibody detection.</description><subject>Antibodies</subject><subject>Antibodies, Viral - analysis</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa cells</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Measles</subject><subject>Measles virus</subject><subject>Measles virus - immunology</subject><subject>Microbiology</subject><subject>Microbiology and Diagnosis</subject><subject>Nucleocapsid</subject><subject>Room temperature</subject><subject>Subacute sclerosing panencephalitis</subject><subject>Techniques used in virology</subject><subject>Virions</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAUhS1EVYaWB2CB5AVil9b_iZejtjCVZvhRS6kQkuU4jnCbxINvIhievi4zDEu8seXv3CPdD6GXlJxQovlpGNomwCkV5YnKP1I-QTMqeVkoRflTNCOEsYJWWj9DzwHuCCGCq_IQHSouuGR8hr7N8Xv_E1_FLjTFx-8WPL4Yfm96XyzDcO8bfNn30xAhptoPI54D2A1uY8JXa-9CGxyeD2OoYxM84DHilbfQ5edNSBMco4PWduBf7O4j9PntxfXZolh-eHd5Nl8WTlA1Fo0kutTKEsGkcjWjrLFNw6raq5bVVUaqkp4JV0mV1yZMcMLzcV5rwYXlR-jNtned4o_Jw2j6AM53nR18nMBURGjBKPlvkHKlmCBlDtJt0KUIkHxr1in0Nm0MJeZRvdmqN1m9UeZRfZ55tSuf6t43-4md68xf77gFZ7s22cHlhr8xzSVXjP2ruYMxpj3mhFJJ_-xQbHmA0f_ac5vujSp5Kc3i9qv5cvtpJRc3K3POHwAcdKRm</recordid><startdate>198306</startdate><enddate>198306</enddate><creator>Rice, George P. A.</creator><creator>Casali, Paolo</creator><creator>Oldstone, Michael B. A.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198306</creationdate><title>A New Solid-Phase Enzyme-Linked Immunosorbent Assay for Specific Antibodies to Measles Virus</title><author>Rice, George P. A. ; Casali, Paolo ; Oldstone, Michael B. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-d509796a04256cb212dadd28be6f2b8796685e24c856109024303333ce99434a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Antibodies</topic><topic>Antibodies, Viral - analysis</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa cells</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Measles</topic><topic>Measles virus</topic><topic>Measles virus - immunology</topic><topic>Microbiology</topic><topic>Microbiology and Diagnosis</topic><topic>Nucleocapsid</topic><topic>Room temperature</topic><topic>Subacute sclerosing panencephalitis</topic><topic>Techniques used in virology</topic><topic>Virions</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rice, George P. A.</creatorcontrib><creatorcontrib>Casali, Paolo</creatorcontrib><creatorcontrib>Oldstone, Michael B. A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rice, George P. A.</au><au>Casali, Paolo</au><au>Oldstone, Michael B. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A New Solid-Phase Enzyme-Linked Immunosorbent Assay for Specific Antibodies to Measles Virus</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1983-06</date><risdate>1983</risdate><volume>147</volume><issue>6</issue><spage>1055</spage><epage>1059</epage><pages>1055-1059</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>A convenient and flexible enzyme-linked immunosorbent assay system for the detection of specific antibodies to measles virus has been developed. In this system infected cells are desiccated on 96-well microtiter plates and stored at room temperature. After incubation of samples to be tested in the cell-coated plates and subsequent washing, bound antibodies are detected with a peroxidase-conjugated staphylococcal protein A probe. After another washing and the addition of the appropriate substrate, the amount of bound probe is estimated by colorimetric analysis. This technique offers several advantages. The need for a purified viral antigen source is obviated. The plates are easily prepared and can be stored for months at room temperature. Major viral epitopes, including surface glycoproteins as well as cytoplasmic viral antigens, are preserved despite desiccation. The method is more sensitive than the conventional means of virus-specific antibody detection.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>6343523</pmid><doi>10.1093/infdis/147.6.1055</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 1983-06, Vol.147 (6), p.1055-1059 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Antibodies Antibodies, Viral - analysis Antibodies, Viral - immunology Antigens, Viral - immunology Biological and medical sciences Enzyme linked immunosorbent assay Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology HeLa cells Humans Immunoenzyme Techniques Measles Measles virus Measles virus - immunology Microbiology Microbiology and Diagnosis Nucleocapsid Room temperature Subacute sclerosing panencephalitis Techniques used in virology Virions Virology Viruses |
| title | A New Solid-Phase Enzyme-Linked Immunosorbent Assay for Specific Antibodies to Measles Virus |
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