Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors

We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M Φs, including antibody-depe...

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Veröffentlicht in:	Journal of immunological methods 1991-03, Vol.137 (1), p.17-25
Hauptverfasser:	Wilson, Cathy M., Gatewood, Janet W., McCormack, Jeffrey M., Walker, William S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Antigen presentation Biological and medical sciences Biotechnology Cell Division - drug effects Cell line Cell Line, Transformed Colony stimulating factor-1 Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Macrophage Macrophage Colony-Stimulating Factor - pharmacology Macrophages - cytology Macrophages - immunology Methods. Procedures. Technologies Mice Mice, Inbred Strains Muramidase - metabolism Phenotype Receptor, Macrophage Colony-Stimulating Factor - analysis RNA-Directed DNA Polymerase - analysis Spleen Spleen - cytology Stem Cells - cytology
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