Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors
We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M Φs, including antibody-depe...
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| Veröffentlicht in: | Journal of immunological methods 1991-03, Vol.137 (1), p.17-25 |
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| container_title | Journal of immunological methods |
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| creator | Wilson, Cathy M. Gatewood, Janet W. McCormack, Jeffrey M. Walker, William S. |
| description | We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M
Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M
Φs, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. It was also possible to immortalize selected clones of splenic M
Φs differing in their constitutive antigen-presenting activities with the retention of the antigen-presenting phenotype in the resultant cell lines. The approach described in this report should be useful in obtaining additional cell lines of M
Φs expressing other phenotypes of interest. |
| doi_str_mv | 10.1016/0022-1759(91)90389-W |
| format | Article |
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Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M
Φs, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. It was also possible to immortalize selected clones of splenic M
Φs differing in their constitutive antigen-presenting activities with the retention of the antigen-presenting phenotype in the resultant cell lines. The approach described in this report should be useful in obtaining additional cell lines of M
Φs expressing other phenotypes of interest.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(91)90389-W</identifier><identifier>PMID: 1707081</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal cells ; Animals ; Antigen presentation ; Biological and medical sciences ; Biotechnology ; Cell Division - drug effects ; Cell line ; Cell Line, Transformed ; Colony stimulating factor-1 ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Macrophage ; Macrophage Colony-Stimulating Factor - pharmacology ; Macrophages - cytology ; Macrophages - immunology ; Methods. Procedures. Technologies ; Mice ; Mice, Inbred Strains ; Muramidase - metabolism ; Phenotype ; Receptor, Macrophage Colony-Stimulating Factor - analysis ; RNA-Directed DNA Polymerase - analysis ; Spleen ; Spleen - cytology ; Stem Cells - cytology</subject><ispartof>Journal of immunological methods, 1991-03, Vol.137 (1), p.17-25</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-8688c4dc93b59d057d21b359570e4e75d65c3765c781d56d86584e6f9286a19f3</citedby><cites>FETCH-LOGICAL-c417t-8688c4dc93b59d057d21b359570e4e75d65c3765c781d56d86584e6f9286a19f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/002217599190389W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4986385$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1707081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilson, Cathy M.</creatorcontrib><creatorcontrib>Gatewood, Janet W.</creatorcontrib><creatorcontrib>McCormack, Jeffrey M.</creatorcontrib><creatorcontrib>Walker, William S.</creatorcontrib><title>Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M
Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M
Φs, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. It was also possible to immortalize selected clones of splenic M
Φs differing in their constitutive antigen-presenting activities with the retention of the antigen-presenting phenotype in the resultant cell lines. The approach described in this report should be useful in obtaining additional cell lines of M
Φs expressing other phenotypes of interest.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Antigen presentation</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Division - drug effects</subject><subject>Cell line</subject><subject>Cell Line, Transformed</subject><subject>Colony stimulating factor-1</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Macrophage</subject><subject>Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Macrophages - cytology</subject><subject>Macrophages - immunology</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Muramidase - metabolism</subject><subject>Phenotype</subject><subject>Receptor, Macrophage Colony-Stimulating Factor - analysis</subject><subject>RNA-Directed DNA Polymerase - analysis</subject><subject>Spleen</subject><subject>Spleen - cytology</subject><subject>Stem Cells - cytology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67j6DxRyENFDa1V35-siyOLHwoIXZY8hk1TPRro7bdKzor_ejDOsN72kAvXUW1VvMfYU4TUCyjcAbdugEualwVcGOm2a63tsg1q1jTIg7rPNHfKQPSrlGwAgSDhjZ6hAgcYNC5fTlPLqxvjLrTHNPA18l9OP9YYPzq8pN4EWmgPNK5_SvhAvy0hz9HxyPqflxu2o8EA53lLgQ04T92Oa63_JaVfBKlEesweDGws9OcVz9vXD-y8Xn5qrzx8vL95dNb5HtTZaau374E23FSaAUKHFbSeMUEA9KRGk8J2qj9IYhAxaCt2THEyrpUMzdOfsxVG39v6-p7LaKRZP4-hmqrNbDb1Bpdr_giihrV6ZCvZHsO5aSqbBLjlOLv-0CPZwBXuw2B4stgbtnyvY61r27KS_304U_hYdba_556e8K96NQ3azj-UO642WnRYVe3vEqJp2Gynb4iPNnkLM5FcbUvz3HL8B7ZikOA</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Wilson, Cathy M.</creator><creator>Gatewood, Janet W.</creator><creator>McCormack, Jeffrey M.</creator><creator>Walker, William S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910301</creationdate><title>Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors</title><author>Wilson, Cathy M. ; Gatewood, Janet W. ; McCormack, Jeffrey M. ; Walker, William S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-8688c4dc93b59d057d21b359570e4e75d65c3765c781d56d86584e6f9286a19f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Antigen presentation</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Division - drug effects</topic><topic>Cell line</topic><topic>Cell Line, Transformed</topic><topic>Colony stimulating factor-1</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Macrophage</topic><topic>Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Macrophages - cytology</topic><topic>Macrophages - immunology</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Muramidase - metabolism</topic><topic>Phenotype</topic><topic>Receptor, Macrophage Colony-Stimulating Factor - analysis</topic><topic>RNA-Directed DNA Polymerase - analysis</topic><topic>Spleen</topic><topic>Spleen - cytology</topic><topic>Stem Cells - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilson, Cathy M.</creatorcontrib><creatorcontrib>Gatewood, Janet W.</creatorcontrib><creatorcontrib>McCormack, Jeffrey M.</creatorcontrib><creatorcontrib>Walker, William S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilson, Cathy M.</au><au>Gatewood, Janet W.</au><au>McCormack, Jeffrey M.</au><au>Walker, William S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>137</volume><issue>1</issue><spage>17</spage><epage>25</epage><pages>17-25</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>We describe a nonviral transformation strategy for the establishment of permanent cell lines derived from the progeny of individual mouse splenic macrophage (M
Φ) progenitors. These colony stimulating factor-1 (CSF-1)-dependent cell lines possess many features of mature M
Φs, including antibody-dependent phagocytic and cellular cytotoxic activities, ability to secrete lysozyme, and expression of the Mac-1 antigen and mRNA for the CSF-1 receptor. It was also possible to immortalize selected clones of splenic M
Φs differing in their constitutive antigen-presenting activities with the retention of the antigen-presenting phenotype in the resultant cell lines. The approach described in this report should be useful in obtaining additional cell lines of M
Φs expressing other phenotypes of interest.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1707081</pmid><doi>10.1016/0022-1759(91)90389-W</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1991-03, Vol.137 (1), p.17-25 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal cells Animals Antigen presentation Biological and medical sciences Biotechnology Cell Division - drug effects Cell line Cell Line, Transformed Colony stimulating factor-1 Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Macrophage Macrophage Colony-Stimulating Factor - pharmacology Macrophages - cytology Macrophages - immunology Methods. Procedures. Technologies Mice Mice, Inbred Strains Muramidase - metabolism Phenotype Receptor, Macrophage Colony-Stimulating Factor - analysis RNA-Directed DNA Polymerase - analysis Spleen Spleen - cytology Stem Cells - cytology |
| title | Immortalization of growth factor-dependent mouse splenic macrophages derived from cloned progenitors |
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