Streptavidin-induced lysis of homologous biotinylated erythrocytes Evidence against the key role of the avidin charge in complement activation via the alternative pathway

It is shown that non-covalent attachment of streptavidin, as well as of avidin, to biotinylated human erythrocytes induces homologous hemolysis by complement. Rabbit antiserum against human C3 is found to inhibit the lysis specifically as compared with non-immune rabbit serum. Efficiency of lysis in...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1991-03, Vol.280 (1), p.112-114
Hauptverfasser:	Muzykantov, Vladimir R., Smirnov, Michael D., Samokhin, Gennady P.
Format:	Artikel
Sprache:	eng
Schlagworte:	alternative pathway alternative pathway of complement APC Avidin Avidin-biotin interaction Bacterial Proteins - pharmacology Biological and medical sciences biotin Biotin - metabolism Cell physiology Complement Complement C3 - immunology Complement Pathway, Alternative complement receptor type 1 CR1 DAF decay accelerating factor erythrocytes Erythrocytes - drug effects Erythrocytes - immunology Fundamental and applied biological sciences. Psychology Hemolysis - drug effects homologous restriction factor HRF Humans Immune Sera Membrane modification Miscellaneous Molecular and cellular biology PBS phosphate-buffered saline plasma membranes sheep erythrocytes sensitized by hemolytic antibody Streptavidin VBS veronal-buffered saline
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!