An experimental artifact in the use of chelating metal ion buffers. Binding of chelators to bovine alpha-lactalbumin

The binding of EDTA and EGTA to bovine alpha-lactalbumin was shown to stabilize the native conformation relative to that characteristic of the metal-free protein (A conformer). Fluorescence titration of the metal-free protein with Ca2+ in the presence of EDTA was markedly influenced by micromolar co...

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Veröffentlicht in:	The Journal of biological chemistry 1983-05, Vol.258 (9), p.5707-5709
Hauptverfasser:	Kronman, M J, Bratcher, S C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Buffers Calcium - metabolism Cattle Edetic Acid - metabolism Egtazic Acid - metabolism Ethylene Glycols - metabolism Kinetics Lactalbumin - metabolism Mathematics Protein Conformation Spectrometry, Fluorescence
Online-Zugang:	Volltext
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