Pteroylpolyglutamates in human milk
Considerable variation exists in reported values for total folate content and the pteroylpolyglutamate (PteGlun) content of human milk. We investigated possible methodological sources of this variation. In two laboratories, milk folate content (with and without folate conjugase) was determined micro...
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| Veröffentlicht in: | The American journal of clinical nutrition 1991-04, Vol.53 (4), p.930-934 |
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| container_title | The American journal of clinical nutrition |
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| creator | O'Connor, DL Tamura, T Picciano, MF |
| description | Considerable variation exists in reported values for total folate content and the pteroylpolyglutamate (PteGlun) content of human milk. We investigated possible methodological sources of this variation. In two laboratories, milk folate content (with and without folate conjugase) was determined microbiologically. No differences in total milk folate or PteGlun (n > 3) content were found between laboratories. PteGlun was found to comprise a significant fraction of total milk folate (28%). Use of rennin did not alter total folate content nor the percent of PteGlun in human milk. Heating (121 °C for 5 min) increased folate concentrations (190%, P < 0.0001), indicating that release of folate from binding protein is necessary for folate utilization by Lactobacillus casei. Although human milk folate conjugase, (FC) activity was approximately one-twentieth that of plasma FC activity, it was not sufficient to autolyze endogenous PteGlun. Thus, microbiological protocols that do not use folate conjugase and do not release folate from binding proteins will seriously underestimate milk folate values. |
| doi_str_mv | 10.1093/ajcn/53.4.930 |
| format | Article |
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We investigated possible methodological sources of this variation. In two laboratories, milk folate content (with and without folate conjugase) was determined microbiologically. No differences in total milk folate or PteGlun (n > 3) content were found between laboratories. PteGlun was found to comprise a significant fraction of total milk folate (28%). Use of rennin did not alter total folate content nor the percent of PteGlun in human milk. Heating (121 °C for 5 min) increased folate concentrations (190%, P < 0.0001), indicating that release of folate from binding protein is necessary for folate utilization by Lactobacillus casei. Although human milk folate conjugase, (FC) activity was approximately one-twentieth that of plasma FC activity, it was not sufficient to autolyze endogenous PteGlun. Thus, microbiological protocols that do not use folate conjugase and do not release folate from binding proteins will seriously underestimate milk folate values.</description><identifier>ISSN: 0002-9165</identifier><identifier>EISSN: 1938-3207</identifier><identifier>DOI: 10.1093/ajcn/53.4.930</identifier><identifier>PMID: 1901195</identifier><identifier>CODEN: AJCNAC</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>ACIDE FOLIQUE ; ACIDO FOLICO ; Biological and medical sciences ; False Negative Reactions ; Female ; folate ; folate conjugase ; Folic Acid - analysis ; Fundamental and applied biological sciences. Psychology ; gamma-Glutamyl Hydrolase - metabolism ; HIDROLASAS ; Human milk ; Humans ; HYDROLASE ; ILLINOIS ; Lactobacillus casei ; LAIT HUMAIN ; LECHE HUMANA ; milk folate ; Milk, Human - chemistry ; Milk, Human - enzymology ; pteroylpolyglutamates ; Pteroylpolyglutamic Acids - analysis ; Streptococcus ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; VALEUR NUTRITIVE ; VALOR NUTRITIVO ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; VITAMINAS ; VITAMINE</subject><ispartof>The American journal of clinical nutrition, 1991-04, Vol.53 (4), p.930-934</ispartof><rights>1991 American Society for Nutrition.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-263c06688480aa26bbbc327ff908fa7a8cd70ac294303add7276e38f3cad5eae3</citedby><cites>FETCH-LOGICAL-c423t-263c06688480aa26bbbc327ff908fa7a8cd70ac294303add7276e38f3cad5eae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5107062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1901195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Connor, DL</creatorcontrib><creatorcontrib>Tamura, T</creatorcontrib><creatorcontrib>Picciano, MF</creatorcontrib><title>Pteroylpolyglutamates in human milk</title><title>The American journal of clinical nutrition</title><addtitle>Am J Clin Nutr</addtitle><description>Considerable variation exists in reported values for total folate content and the pteroylpolyglutamate (PteGlun) content of human milk. We investigated possible methodological sources of this variation. In two laboratories, milk folate content (with and without folate conjugase) was determined microbiologically. No differences in total milk folate or PteGlun (n > 3) content were found between laboratories. PteGlun was found to comprise a significant fraction of total milk folate (28%). Use of rennin did not alter total folate content nor the percent of PteGlun in human milk. Heating (121 °C for 5 min) increased folate concentrations (190%, P < 0.0001), indicating that release of folate from binding protein is necessary for folate utilization by Lactobacillus casei. Although human milk folate conjugase, (FC) activity was approximately one-twentieth that of plasma FC activity, it was not sufficient to autolyze endogenous PteGlun. Thus, microbiological protocols that do not use folate conjugase and do not release folate from binding proteins will seriously underestimate milk folate values.</description><subject>ACIDE FOLIQUE</subject><subject>ACIDO FOLICO</subject><subject>Biological and medical sciences</subject><subject>False Negative Reactions</subject><subject>Female</subject><subject>folate</subject><subject>folate conjugase</subject><subject>Folic Acid - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Glutamyl Hydrolase - metabolism</subject><subject>HIDROLASAS</subject><subject>Human milk</subject><subject>Humans</subject><subject>HYDROLASE</subject><subject>ILLINOIS</subject><subject>Lactobacillus casei</subject><subject>LAIT HUMAIN</subject><subject>LECHE HUMANA</subject><subject>milk folate</subject><subject>Milk, Human - chemistry</subject><subject>Milk, Human - enzymology</subject><subject>pteroylpolyglutamates</subject><subject>Pteroylpolyglutamic Acids - analysis</subject><subject>Streptococcus</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>VALEUR NUTRITIVE</subject><subject>VALOR NUTRITIVO</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>VITAMINAS</subject><subject>VITAMINE</subject><issn>0002-9165</issn><issn>1938-3207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLxDAURoMoOo4u3QjCgOKu403S5rGUwRcICjrrcJumGu1jTFph_r0dOujK1V2cw8flEHJCYU5B8yv8sM1VxufpXHPYIROquUo4A7lLJgDAEk1FdkAOY_wAoCxVYp_sUw2U6mxCzp87F9p1tWqr9VvVd1hj5-LMN7P3vsZmVvvq84jslVhFd7y9U7K8vXld3CePT3cPi-vHxKaMdwkT3IIQSqUKEJnI89xyJstSgypRorKFBLRMpxw4FoVkUjiuSm6xyBw6PiWX4-4qtF-9i52pfbSuqrBxbR-NglQDcDGIySja0MYYXGlWwdcY1oaC2UQxmygm4yY1Q5TBP9sO93ntij97rDDwiy3HaLEqAzbWx18toyBBsEE7HbUSW4NvYVCWL5oyIbkaoByhGwp9exdMtN411hU-ONuZovX_fPcDIBOEqg</recordid><startdate>19910401</startdate><enddate>19910401</enddate><creator>O'Connor, DL</creator><creator>Tamura, T</creator><creator>Picciano, MF</creator><general>Elsevier Inc</general><general>American Society for Clinical Nutrition</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910401</creationdate><title>Pteroylpolyglutamates in human milk</title><author>O'Connor, DL ; Tamura, T ; Picciano, MF</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-263c06688480aa26bbbc327ff908fa7a8cd70ac294303add7276e38f3cad5eae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>ACIDE FOLIQUE</topic><topic>ACIDO FOLICO</topic><topic>Biological and medical sciences</topic><topic>False Negative Reactions</topic><topic>Female</topic><topic>folate</topic><topic>folate conjugase</topic><topic>Folic Acid - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Glutamyl Hydrolase - metabolism</topic><topic>HIDROLASAS</topic><topic>Human milk</topic><topic>Humans</topic><topic>HYDROLASE</topic><topic>ILLINOIS</topic><topic>Lactobacillus casei</topic><topic>LAIT HUMAIN</topic><topic>LECHE HUMANA</topic><topic>milk folate</topic><topic>Milk, Human - chemistry</topic><topic>Milk, Human - enzymology</topic><topic>pteroylpolyglutamates</topic><topic>Pteroylpolyglutamic Acids - analysis</topic><topic>Streptococcus</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>VALEUR NUTRITIVE</topic><topic>VALOR NUTRITIVO</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>VITAMINAS</topic><topic>VITAMINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Connor, DL</creatorcontrib><creatorcontrib>Tamura, T</creatorcontrib><creatorcontrib>Picciano, MF</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of clinical nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Connor, DL</au><au>Tamura, T</au><au>Picciano, MF</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pteroylpolyglutamates in human milk</atitle><jtitle>The American journal of clinical nutrition</jtitle><addtitle>Am J Clin Nutr</addtitle><date>1991-04-01</date><risdate>1991</risdate><volume>53</volume><issue>4</issue><spage>930</spage><epage>934</epage><pages>930-934</pages><issn>0002-9165</issn><eissn>1938-3207</eissn><coden>AJCNAC</coden><abstract>Considerable variation exists in reported values for total folate content and the pteroylpolyglutamate (PteGlun) content of human milk. We investigated possible methodological sources of this variation. In two laboratories, milk folate content (with and without folate conjugase) was determined microbiologically. No differences in total milk folate or PteGlun (n > 3) content were found between laboratories. PteGlun was found to comprise a significant fraction of total milk folate (28%). Use of rennin did not alter total folate content nor the percent of PteGlun in human milk. Heating (121 °C for 5 min) increased folate concentrations (190%, P < 0.0001), indicating that release of folate from binding protein is necessary for folate utilization by Lactobacillus casei. Although human milk folate conjugase, (FC) activity was approximately one-twentieth that of plasma FC activity, it was not sufficient to autolyze endogenous PteGlun. Thus, microbiological protocols that do not use folate conjugase and do not release folate from binding proteins will seriously underestimate milk folate values.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>1901195</pmid><doi>10.1093/ajcn/53.4.930</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ACIDE FOLIQUE ACIDO FOLICO Biological and medical sciences False Negative Reactions Female folate folate conjugase Folic Acid - analysis Fundamental and applied biological sciences. Psychology gamma-Glutamyl Hydrolase - metabolism HIDROLASAS Human milk Humans HYDROLASE ILLINOIS Lactobacillus casei LAIT HUMAIN LECHE HUMANA milk folate Milk, Human - chemistry Milk, Human - enzymology pteroylpolyglutamates Pteroylpolyglutamic Acids - analysis Streptococcus TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS VALEUR NUTRITIVE VALOR NUTRITIVO Vertebrates: anatomy and physiology, studies on body, several organs or systems VITAMINAS VITAMINE |
| title | Pteroylpolyglutamates in human milk |
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