The distribution of cerebral expression of the transferrin gene is species specific
Various plasma proteins, for example, transferrin, are synthesized not only in the liver, but also in the brain. The proportion of transferrin mRNA in total RNA from different regions of brains from various mammalian species was studied by Northern blot analysis. Absolute amounts of transferrin mRNA...
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| Veröffentlicht in: | The Journal of biological chemistry 1991-04, Vol.266 (10), p.6201-6208 |
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| creator | Tu, G F Achen, M G Aldred, A R Southwell, B R Schreiber, G |
| description | Various plasma proteins, for example, transferrin, are synthesized not only in the liver, but also in the brain. The proportion of transferrin mRNA in total RNA from different regions of brains from various mammalian species was studied by Northern blot analysis. Absolute amounts of transferrin mRNA were determined in brain, choroid plexus, and liver from rats, sheep, and pigs by hybridization in solution followed by ribonuclease protection assay. Corrections for differences in yields of RNA were made using internal RNA standards. Large proportions of transferrin mRNA in total RNA and high absolute levels of transferrin mRNA in choroid plexus were found only in rats. Small proportions of transferrin mRNA were observed in RNA from choroid plexus from mice, dogs, and rabbits, while no transferrin mRNA at all was detected in choroid plexus from humans, sheep, pigs, cows, and guinea pigs. In further analysis of sheep and pigs, various amounts of transferrin mRNA were found in many parts of the brain, in contrast to the absence of transferrin mRNA from choroid plexus. In conclusion, a striking species specificity was observed for the pattern of cerebral expression of the transferrin gene. |
| doi_str_mv | 10.1016/S0021-9258(18)38104-3 |
| format | Article |
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The proportion of transferrin mRNA in total RNA from different regions of brains from various mammalian species was studied by Northern blot analysis. Absolute amounts of transferrin mRNA were determined in brain, choroid plexus, and liver from rats, sheep, and pigs by hybridization in solution followed by ribonuclease protection assay. Corrections for differences in yields of RNA were made using internal RNA standards. Large proportions of transferrin mRNA in total RNA and high absolute levels of transferrin mRNA in choroid plexus were found only in rats. Small proportions of transferrin mRNA were observed in RNA from choroid plexus from mice, dogs, and rabbits, while no transferrin mRNA at all was detected in choroid plexus from humans, sheep, pigs, cows, and guinea pigs. In further analysis of sheep and pigs, various amounts of transferrin mRNA were found in many parts of the brain, in contrast to the absence of transferrin mRNA from choroid plexus. In conclusion, a striking species specificity was observed for the pattern of cerebral expression of the transferrin gene.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)38104-3</identifier><identifier>PMID: 1848850</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Brain Chemistry ; Cattle ; CERDO ; CEREBRO ; Choroid Plexus - chemistry ; Dogs ; ENCEPHALE ; Endoribonucleases ; EXPRESION GENICA ; EXPRESSION DES GENES ; FOIE ; Fundamental and applied biological sciences. Psychology ; Gene expression ; GLOBULINAS ; GLOBULINE ; Guinea Pigs ; HIGADO ; Humans ; Liver - chemistry ; MENINGE ; MENINGES ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; NUCLEOTIDE ; NUCLEOTIDOS ; OVIN ; OVINOS ; PORCIN ; Rabbits ; RAT ; RATA ; Rats ; Ribonuclease H ; RNA, Messenger - analysis ; Sheep ; Species Specificity ; Swine ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; Transferrin - genetics ; Transferrin - metabolism</subject><ispartof>The Journal of biological chemistry, 1991-04, Vol.266 (10), p.6201-6208</ispartof><rights>1991 © 1991 ASBMB. 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The proportion of transferrin mRNA in total RNA from different regions of brains from various mammalian species was studied by Northern blot analysis. Absolute amounts of transferrin mRNA were determined in brain, choroid plexus, and liver from rats, sheep, and pigs by hybridization in solution followed by ribonuclease protection assay. Corrections for differences in yields of RNA were made using internal RNA standards. Large proportions of transferrin mRNA in total RNA and high absolute levels of transferrin mRNA in choroid plexus were found only in rats. Small proportions of transferrin mRNA were observed in RNA from choroid plexus from mice, dogs, and rabbits, while no transferrin mRNA at all was detected in choroid plexus from humans, sheep, pigs, cows, and guinea pigs. In further analysis of sheep and pigs, various amounts of transferrin mRNA were found in many parts of the brain, in contrast to the absence of transferrin mRNA from choroid plexus. In conclusion, a striking species specificity was observed for the pattern of cerebral expression of the transferrin gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Brain Chemistry</subject><subject>Cattle</subject><subject>CERDO</subject><subject>CEREBRO</subject><subject>Choroid Plexus - chemistry</subject><subject>Dogs</subject><subject>ENCEPHALE</subject><subject>Endoribonucleases</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FOIE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>GLOBULINAS</subject><subject>GLOBULINE</subject><subject>Guinea Pigs</subject><subject>HIGADO</subject><subject>Humans</subject><subject>Liver - chemistry</subject><subject>MENINGE</subject><subject>MENINGES</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>PORCIN</subject><subject>Rabbits</subject><subject>RAT</subject><subject>RATA</subject><subject>Rats</subject><subject>Ribonuclease H</subject><subject>RNA, Messenger - analysis</subject><subject>Sheep</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>Transferrin - genetics</subject><subject>Transferrin - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpSDdpv0AhYCgtycGNRn9s6VRCSJtCoIdNIDchy6NdFa-9lbxp-u2jjU1zjC4Der95M3oi5AToV6BQnS8pZVBqJtUpqDOugIqSvyELoIqXXML9W7L4j7wjRyn9pvkIDYfkEJRQStIFWd6usWhDGmNodmMY-mLwhcOITbRdgY_biCnN12NGx2j75DHG0Bcr7LEIqUhbdAHn6oN7Tw687RJ-mOsxuft-dXt5Xd78-vHz8uKmdBLkmHesGbXKK4lY1Rrbilc1amctSN-2XFWiZrbmwnrmgUtFuQOH3kKjm9zMj8mXyXcbhz87TKPZhOSw62yPwy4ZRYXSXIhXQZC65lTtHeUEujikFNGbbQwbG_8ZoGafunlO3ewjNaDMc-qG576TecCu2WD70jXFnPXPs26Ts53PIbqQXjBdg9BMZ-7TxK3Dav03RDRNGNwaN4ZV1X6HilHI1MeJ8nYwdhWz091SA1O6Zln8NomYk38IGE3Kn9M7bLOdG007hFce8wQtuLI1</recordid><startdate>19910405</startdate><enddate>19910405</enddate><creator>Tu, G F</creator><creator>Achen, M G</creator><creator>Aldred, A R</creator><creator>Southwell, B R</creator><creator>Schreiber, G</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19910405</creationdate><title>The distribution of cerebral expression of the transferrin gene is species specific</title><author>Tu, G F ; Achen, M G ; Aldred, A R ; Southwell, B R ; Schreiber, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-35720a8f85ee679ed6367e9caa15fdd386472a734af2f135803c1cefa1b9b3573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Brain Chemistry</topic><topic>Cattle</topic><topic>CERDO</topic><topic>CEREBRO</topic><topic>Choroid Plexus - chemistry</topic><topic>Dogs</topic><topic>ENCEPHALE</topic><topic>Endoribonucleases</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FOIE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>GLOBULINAS</topic><topic>GLOBULINE</topic><topic>Guinea Pigs</topic><topic>HIGADO</topic><topic>Humans</topic><topic>Liver - chemistry</topic><topic>MENINGE</topic><topic>MENINGES</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>PORCIN</topic><topic>Rabbits</topic><topic>RAT</topic><topic>RATA</topic><topic>Rats</topic><topic>Ribonuclease H</topic><topic>RNA, Messenger - analysis</topic><topic>Sheep</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>Transferrin - genetics</topic><topic>Transferrin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tu, G F</creatorcontrib><creatorcontrib>Achen, M G</creatorcontrib><creatorcontrib>Aldred, A R</creatorcontrib><creatorcontrib>Southwell, B R</creatorcontrib><creatorcontrib>Schreiber, G</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tu, G F</au><au>Achen, M G</au><au>Aldred, A R</au><au>Southwell, B R</au><au>Schreiber, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The distribution of cerebral expression of the transferrin gene is species specific</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-04-05</date><risdate>1991</risdate><volume>266</volume><issue>10</issue><spage>6201</spage><epage>6208</epage><pages>6201-6208</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Various plasma proteins, for example, transferrin, are synthesized not only in the liver, but also in the brain. The proportion of transferrin mRNA in total RNA from different regions of brains from various mammalian species was studied by Northern blot analysis. Absolute amounts of transferrin mRNA were determined in brain, choroid plexus, and liver from rats, sheep, and pigs by hybridization in solution followed by ribonuclease protection assay. Corrections for differences in yields of RNA were made using internal RNA standards. Large proportions of transferrin mRNA in total RNA and high absolute levels of transferrin mRNA in choroid plexus were found only in rats. Small proportions of transferrin mRNA were observed in RNA from choroid plexus from mice, dogs, and rabbits, while no transferrin mRNA at all was detected in choroid plexus from humans, sheep, pigs, cows, and guinea pigs. In further analysis of sheep and pigs, various amounts of transferrin mRNA were found in many parts of the brain, in contrast to the absence of transferrin mRNA from choroid plexus. In conclusion, a striking species specificity was observed for the pattern of cerebral expression of the transferrin gene.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>1848850</pmid><doi>10.1016/S0021-9258(18)38104-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1991-04, Vol.266 (10), p.6201-6208 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals ARN MENSAJERO ARN MESSAGER Base Sequence Biological and medical sciences Blotting, Northern Brain Chemistry Cattle CERDO CEREBRO Choroid Plexus - chemistry Dogs ENCEPHALE Endoribonucleases EXPRESION GENICA EXPRESSION DES GENES FOIE Fundamental and applied biological sciences. Psychology Gene expression GLOBULINAS GLOBULINE Guinea Pigs HIGADO Humans Liver - chemistry MENINGE MENINGES Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data NUCLEOTIDE NUCLEOTIDOS OVIN OVINOS PORCIN Rabbits RAT RATA Rats Ribonuclease H RNA, Messenger - analysis Sheep Species Specificity Swine TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Transferrin - genetics Transferrin - metabolism |
| title | The distribution of cerebral expression of the transferrin gene is species specific |
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