[25] Vectors for expression of cloned genes in yeast: Regulation, overproduction, and underproduction

It is necessary to separate a gene from its own promoter to control expression of its product in different conditions or to produce the product to a greater or lesser degree than normal. This chapter discusses the yeast transcription cassettes of several types used for the expression of cloned genes...

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Veröffentlicht in:	Methods in Enzymology 1991, Vol.194, p.373-388
Hauptverfasser:	Schneider, Jane C., Guarente, Leonard
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cloning, Molecular - methods DNA Replication Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Fungal Genes, Fungal Genetic engineering Genetic Markers Genetic technics Genetic Vectors Methods. Procedures. Technologies Plasmids Restriction Mapping Saccharomyces cerevisiae - genetics Transcription, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
Online-Zugang:	Volltext
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