Temperature-jump kinetics of the dC-G-T-G-A-A-T-T-C-G-C-G double helix containing a G·T base pair and the dC-G-C-A-G-A-A-T-T-C-G-C-G double helix containing an extra adenine
The kinetics of helix formation were investigated using the temperature‐jump technique for the following two molecules: dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing a G·T base pair(the G·T 12‐mer), and dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing an extra aden...
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| Veröffentlicht in: | Biopolymers 1983-04, Vol.22 (4), p.1235-1246 |
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| creator | Chu, Y. Gloria Tinoco Jr, Ignacio |
| description | The kinetics of helix formation were investigated using the temperature‐jump technique for the following two molecules: dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing a G·T base pair(the G·T 12‐mer), and dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing an extra adenine (the 13‐mer). When data were analyzed in an all‐or‐none model, the activation energy for the helix association process was 22 ± 4 kcal/mol for the G·T 12‐mer and 16 ± 7 kcal/mol for the 13‐mer. The activation energy for the helix‐dissociation process was 68 ± 2 kcal/mol for the G·T 12‐mer and 74 ± 3 kcal/mol for the 13‐mer. Rate constants for recombination were near 105s−1M−1 in the temperature range from 32 to 47°C; for the dissociation process, the rate constants varied from 1s−1 near 32°C to 130s−1 near 47°C. Possible effects of hairpin loops and fraying ends on the above data are discussed. |
| doi_str_mv | 10.1002/bip.360220415 |
| format | Article |
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Gloria ; Tinoco Jr, Ignacio</creator><creatorcontrib>Chu, Y. Gloria ; Tinoco Jr, Ignacio ; Univ. of California, Berkeley</creatorcontrib><description>The kinetics of helix formation were investigated using the temperature‐jump technique for the following two molecules: dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing a G·T base pair(the G·T 12‐mer), and dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing an extra adenine (the 13‐mer). When data were analyzed in an all‐or‐none model, the activation energy for the helix association process was 22 ± 4 kcal/mol for the G·T 12‐mer and 16 ± 7 kcal/mol for the 13‐mer. The activation energy for the helix‐dissociation process was 68 ± 2 kcal/mol for the G·T 12‐mer and 74 ± 3 kcal/mol for the 13‐mer. Rate constants for recombination were near 105s−1M−1 in the temperature range from 32 to 47°C; for the dissociation process, the rate constants varied from 1s−1 near 32°C to 130s−1 near 47°C. Possible effects of hairpin loops and fraying ends on the above data are discussed.</description><identifier>ISSN: 0006-3525</identifier><identifier>EISSN: 1097-0282</identifier><identifier>DOI: 10.1002/bip.360220415</identifier><identifier>PMID: 6850062</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987) ; ACTIVATION ENERGY ; Adenine ; CHEMICAL REACTION KINETICS ; DATA ; DATA ANALYSIS ; ENERGY ; EXPERIMENTAL DATA ; INFORMATION ; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ; KINETICS ; MATHEMATICAL MODELS ; MEDIUM TEMPERATURE ; Nucleic Acid Conformation ; NUCLEOTIDES ; NUMERICAL DATA ; Oligodeoxyribonucleotides ; Oligonucleotides ; ORGANIC COMPOUNDS ; REACTION KINETICS ; Structure-Activity Relationship ; Temperature ; TEMPERATURE DEPENDENCE</subject><ispartof>Biopolymers, 1983-04, Vol.22 (4), p.1235-1246</ispartof><rights>Copyright © 1983 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3165-6fb652c5c2c1e7140cddb0014fe48acd254524f3ae89d3f17e86720accfb1f023</citedby><cites>FETCH-LOGICAL-c3165-6fb652c5c2c1e7140cddb0014fe48acd254524f3ae89d3f17e86720accfb1f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbip.360220415$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbip.360220415$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,885,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6850062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5965492$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Chu, Y. Gloria</creatorcontrib><creatorcontrib>Tinoco Jr, Ignacio</creatorcontrib><creatorcontrib>Univ. of California, Berkeley</creatorcontrib><title>Temperature-jump kinetics of the dC-G-T-G-A-A-T-T-C-G-C-G double helix containing a G·T base pair and the dC-G-C-A-G-A-A-T-T-C-G-C-G double helix containing an extra adenine</title><title>Biopolymers</title><addtitle>Biopolymers</addtitle><description>The kinetics of helix formation were investigated using the temperature‐jump technique for the following two molecules: dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing a G·T base pair(the G·T 12‐mer), and dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing an extra adenine (the 13‐mer). When data were analyzed in an all‐or‐none model, the activation energy for the helix association process was 22 ± 4 kcal/mol for the G·T 12‐mer and 16 ± 7 kcal/mol for the 13‐mer. The activation energy for the helix‐dissociation process was 68 ± 2 kcal/mol for the G·T 12‐mer and 74 ± 3 kcal/mol for the 13‐mer. Rate constants for recombination were near 105s−1M−1 in the temperature range from 32 to 47°C; for the dissociation process, the rate constants varied from 1s−1 near 32°C to 130s−1 near 47°C. Possible effects of hairpin loops and fraying ends on the above data are discussed.</description><subject>400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)</subject><subject>ACTIVATION ENERGY</subject><subject>Adenine</subject><subject>CHEMICAL REACTION KINETICS</subject><subject>DATA</subject><subject>DATA ANALYSIS</subject><subject>ENERGY</subject><subject>EXPERIMENTAL DATA</subject><subject>INFORMATION</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>KINETICS</subject><subject>MATHEMATICAL MODELS</subject><subject>MEDIUM TEMPERATURE</subject><subject>Nucleic Acid Conformation</subject><subject>NUCLEOTIDES</subject><subject>NUMERICAL DATA</subject><subject>Oligodeoxyribonucleotides</subject><subject>Oligonucleotides</subject><subject>ORGANIC COMPOUNDS</subject><subject>REACTION KINETICS</subject><subject>Structure-Activity Relationship</subject><subject>Temperature</subject><subject>TEMPERATURE DEPENDENCE</subject><issn>0006-3525</issn><issn>1097-0282</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkU2LFDEQhoMo6-zq0aMQPHjrtfLZPcd10HFhUcHWPYZ0utrJbn9t0o2zf2qv3v1lZphh9CRIUYSknjwUvIS8YHDOAPibyo_nQgPnIJl6RBYMlnkGvOCPyQIAdCYUV0_JaYw3AFIKBifkRBcqjfiCPJTYjRjsNAfMbuZupLe-x8m7SIeGThuk9SpbZ2Xqi1Rlqt09Na2HuWqRbrD1W-qGfrK-9_13aun618-SVjYiHa0P1Pb1H9MqWf7D1VPcTsFSW2N6wGfkSWPbiM8P5xn5-v5dufqQXX1aX64urjInmFaZbiqtuFOOO4Y5k-DqugJgskFZWFdzJRWXjbBYLGvRsBwLnXOwzjUVa4CLM_Jq7x3i5E10fkK3SXv16CajllrJ5Q56vYfGMNzNGCfT-eiwbW2PwxxNATIvNBMJzPagC0OMARszBt_ZcG8YmF2IJoVojiEm_uVBPFcd1kf6kFqa5_v5D9_i_b9l5u3l57_Nh018nHB7_GnDrdG5yJW5_rg2376U4pqVyjDxG3QZs04</recordid><startdate>198304</startdate><enddate>198304</enddate><creator>Chu, Y. Gloria</creator><creator>Tinoco Jr, Ignacio</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198304</creationdate><title>Temperature-jump kinetics of the dC-G-T-G-A-A-T-T-C-G-C-G double helix containing a G·T base pair and the dC-G-C-A-G-A-A-T-T-C-G-C-G double helix containing an extra adenine</title><author>Chu, Y. Gloria ; Tinoco Jr, Ignacio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3165-6fb652c5c2c1e7140cddb0014fe48acd254524f3ae89d3f17e86720accfb1f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)</topic><topic>ACTIVATION ENERGY</topic><topic>Adenine</topic><topic>CHEMICAL REACTION KINETICS</topic><topic>DATA</topic><topic>DATA ANALYSIS</topic><topic>ENERGY</topic><topic>EXPERIMENTAL DATA</topic><topic>INFORMATION</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>KINETICS</topic><topic>MATHEMATICAL MODELS</topic><topic>MEDIUM TEMPERATURE</topic><topic>Nucleic Acid Conformation</topic><topic>NUCLEOTIDES</topic><topic>NUMERICAL DATA</topic><topic>Oligodeoxyribonucleotides</topic><topic>Oligonucleotides</topic><topic>ORGANIC COMPOUNDS</topic><topic>REACTION KINETICS</topic><topic>Structure-Activity Relationship</topic><topic>Temperature</topic><topic>TEMPERATURE DEPENDENCE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chu, Y. Gloria</creatorcontrib><creatorcontrib>Tinoco Jr, Ignacio</creatorcontrib><creatorcontrib>Univ. of California, Berkeley</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biopolymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chu, Y. Gloria</au><au>Tinoco Jr, Ignacio</au><aucorp>Univ. of California, Berkeley</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temperature-jump kinetics of the dC-G-T-G-A-A-T-T-C-G-C-G double helix containing a G·T base pair and the dC-G-C-A-G-A-A-T-T-C-G-C-G double helix containing an extra adenine</atitle><jtitle>Biopolymers</jtitle><addtitle>Biopolymers</addtitle><date>1983-04</date><risdate>1983</risdate><volume>22</volume><issue>4</issue><spage>1235</spage><epage>1246</epage><pages>1235-1246</pages><issn>0006-3525</issn><eissn>1097-0282</eissn><abstract>The kinetics of helix formation were investigated using the temperature‐jump technique for the following two molecules: dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing a G·T base pair(the G·T 12‐mer), and dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G, which forms a double helix containing an extra adenine (the 13‐mer). When data were analyzed in an all‐or‐none model, the activation energy for the helix association process was 22 ± 4 kcal/mol for the G·T 12‐mer and 16 ± 7 kcal/mol for the 13‐mer. The activation energy for the helix‐dissociation process was 68 ± 2 kcal/mol for the G·T 12‐mer and 74 ± 3 kcal/mol for the 13‐mer. Rate constants for recombination were near 105s−1M−1 in the temperature range from 32 to 47°C; for the dissociation process, the rate constants varied from 1s−1 near 32°C to 130s−1 near 47°C. Possible effects of hairpin loops and fraying ends on the above data are discussed.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>6850062</pmid><doi>10.1002/bip.360220415</doi><tpages>12</tpages></addata></record> |
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| ispartof | Biopolymers, 1983-04, Vol.22 (4), p.1235-1246 |
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| subjects | 400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987) ACTIVATION ENERGY Adenine CHEMICAL REACTION KINETICS DATA DATA ANALYSIS ENERGY EXPERIMENTAL DATA INFORMATION INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY KINETICS MATHEMATICAL MODELS MEDIUM TEMPERATURE Nucleic Acid Conformation NUCLEOTIDES NUMERICAL DATA Oligodeoxyribonucleotides Oligonucleotides ORGANIC COMPOUNDS REACTION KINETICS Structure-Activity Relationship Temperature TEMPERATURE DEPENDENCE |
| title | Temperature-jump kinetics of the dC-G-T-G-A-A-T-T-C-G-C-G double helix containing a G·T base pair and the dC-G-C-A-G-A-A-T-T-C-G-C-G double helix containing an extra adenine |
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