High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives
A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized anal...
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| Veröffentlicht in: | Analytical biochemistry 1983, Vol.128 (1), p.191-195 |
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| container_end_page | 195 |
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| container_issue | 1 |
| container_start_page | 191 |
| container_title | Analytical biochemistry |
| container_volume | 128 |
| creator | Kumar, A.Ashok Kempton, Robert J. Anstead, Gregory M. Price, Elmer M. Freisheim, James H. |
| description | A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described. |
| doi_str_mv | 10.1016/0003-2697(83)90362-7 |
| format | Article |
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The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(83)90362-7</identifier><identifier>PMID: 6405656</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Chromatography, High Pressure Liquid - methods ; Dansyl Compounds - isolation & purification ; Fluorescence ; Glutamates ; lysine ; Lysine - analogs & derivatives ; methotrexate ; Methotrexate - analogs & derivatives ; Methotrexate - isolation & purification ; ornithine ; Ornithine - analogs & derivatives</subject><ispartof>Analytical biochemistry, 1983, Vol.128 (1), p.191-195</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-3fdc0b83291d974434109a4fae7dd2eed85f4d5e2faa058917b6f9dfaabee43</citedby><cites>FETCH-LOGICAL-c388t-3fdc0b83291d974434109a4fae7dd2eed85f4d5e2faa058917b6f9dfaabee43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269783903627$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6405656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, A.Ashok</creatorcontrib><creatorcontrib>Kempton, Robert J.</creatorcontrib><creatorcontrib>Anstead, Gregory M.</creatorcontrib><creatorcontrib>Price, Elmer M.</creatorcontrib><creatorcontrib>Freisheim, James H.</creatorcontrib><title>High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. The methotrexate analogs containing a lysine or an ornithine residue in place of a terminal glutamate residue together with their respective dansyl derivatives were purified in milligram quantities by the procedures described.</description><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dansyl Compounds - isolation & purification</subject><subject>Fluorescence</subject><subject>Glutamates</subject><subject>lysine</subject><subject>Lysine - analogs & derivatives</subject><subject>methotrexate</subject><subject>Methotrexate - analogs & derivatives</subject><subject>Methotrexate - isolation & purification</subject><subject>ornithine</subject><subject>Ornithine - analogs & derivatives</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcGKFDEQDeKyjqt_oJCT6KHXpJNO0hdBFnWFhT3oPWSSynSkO5lNMoNz9sc34wx7VCgoqurVK-o9hN5Qck0JFR8JIazrxSjfK_ZhJEz0nXyGVpSMoiOMjM_R6gnyAr0s5RchlPJBXKJLwckgBrFCf27DZuq2kH3Ki4kW8BwedsFhO-W0mJo22WynA04eL1CnVDP8NhWwiWZOm4JtitWEGOIGV8hLaG08H0qIgFNuEUOdjoWJDtcJQsbOxHKYsYMc9qaGPZRX6MKbucDrc75CP75--Xlz293df_t-8_mus0yp2jHvLFkr1o_UjZJzxturhnsD0rkewKnBczdA740hgxqpXAs_ulatATi7Qu9OrNucHnZQql5CsTDPJkLaFa0Il1IO_X-BlA2McTU2ID8BbU6lZPB6m8Ni8kFToo8W6aP--qi_Vkz_tUjLtvb2zL9bL-Cels6etPmn0xyaFvsAWRcboFnjQgZbtUvh3wceASi4pMA</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Kumar, A.Ashok</creator><creator>Kempton, Robert J.</creator><creator>Anstead, Gregory M.</creator><creator>Price, Elmer M.</creator><creator>Freisheim, James H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1983</creationdate><title>High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives</title><author>Kumar, A.Ashok ; Kempton, Robert J. ; Anstead, Gregory M. ; Price, Elmer M. ; Freisheim, James H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-3fdc0b83291d974434109a4fae7dd2eed85f4d5e2faa058917b6f9dfaabee43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dansyl Compounds - isolation & purification</topic><topic>Fluorescence</topic><topic>Glutamates</topic><topic>lysine</topic><topic>Lysine - analogs & derivatives</topic><topic>methotrexate</topic><topic>Methotrexate - analogs & derivatives</topic><topic>Methotrexate - isolation & purification</topic><topic>ornithine</topic><topic>Ornithine - analogs & derivatives</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, A.Ashok</creatorcontrib><creatorcontrib>Kempton, Robert J.</creatorcontrib><creatorcontrib>Anstead, Gregory M.</creatorcontrib><creatorcontrib>Price, Elmer M.</creatorcontrib><creatorcontrib>Freisheim, James H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, A.Ashok</au><au>Kempton, Robert J.</au><au>Anstead, Gregory M.</au><au>Price, Elmer M.</au><au>Freisheim, James H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1983</date><risdate>1983</risdate><volume>128</volume><issue>1</issue><spage>191</spage><epage>195</epage><pages>191-195</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A procedure utilizing a reverse-phase semipreparative high-performance liquid chromatography column and a binary solvent system consisting of trifluoroacetic acid and 1-propanol has been developed for the semipreparative scale purification and analytical identification of four newly synthesized analogs of methotrexate. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1983, Vol.128 (1), p.191-195 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80477752 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Chromatography, High Pressure Liquid - methods Dansyl Compounds - isolation & purification Fluorescence Glutamates lysine Lysine - analogs & derivatives methotrexate Methotrexate - analogs & derivatives Methotrexate - isolation & purification ornithine Ornithine - analogs & derivatives |
| title | High-performance liquid chromatography of methotrexate analogs containing terminal lysine or ornithine and their dansyl derivatives |
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