Determination of malonaldehyde in biological materials by high-pressure liquid chromatography
An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a μBondapak C 18 column, and me...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 1983, Vol.128 (1), p.240-244 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 244 |
|---|---|
| container_issue | 1 |
| container_start_page | 240 |
| container_title | Analytical biochemistry |
| container_volume | 128 |
| creator | Bird, R.P. Hung, Silas S.O. Hadley, M. Draper, H.H. |
| description | An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a μBondapak C
18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1–2 μg of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm. |
| doi_str_mv | 10.1016/0003-2697(83)90371-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80475697</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269783903718</els_id><sourcerecordid>80475697</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-308ce7972afefd3b9dad848a0592d0a69ff606bd93a76a96bd2caf2f913245253</originalsourceid><addsrcrecordid>eNp9kFFL5DAQx8Oh6LrnNzihT6IPPSdNmzYvgninHgi-6OMR0mSyjbTNmrTCfnuz7uKjTzPw_80M8yPkF4XfFCi_AgCWF1zUFw27FMBqmjc_yIKC4DkwEAdk8YUck5MYXwEoLSt-RI54U_Ja8AX5_wcnDIMb1eT8mHmbDar3o-oNdhuDmRuz1vner5xWfcoS7FQfs3aTdW7V5euAMc4Bs969zc5kugs-UX4V1Lrb_CSHNtF4uq9L8nL39_n2IX98uv93e_OYa1bVU86g0ViLulAWrWGtMMo0ZaOgEoUBxYW1HHhrBFM1VyJ1hVa2sIKyoqyKii3J-W7vOvi3GeMkBxc19r0a0c9RNlDWVdKQwHIH6uBjDGjlOrhBhY2kILdW5VaZ3CqTDZOfVlOzJGf7_XM7oPka2mtM-fUux_Tku8Mgo3Y4ajQuoJ6k8e77Ax8UyYiW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80475697</pqid></control><display><type>article</type><title>Determination of malonaldehyde in biological materials by high-pressure liquid chromatography</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Bird, R.P. ; Hung, Silas S.O. ; Hadley, M. ; Draper, H.H.</creator><creatorcontrib>Bird, R.P. ; Hung, Silas S.O. ; Hadley, M. ; Draper, H.H.</creatorcontrib><description>An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a μBondapak C
18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1–2 μg of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(83)90371-8</identifier><identifier>PMID: 6846796</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animal Feed - analysis ; Chromatography, High Pressure Liquid - methods ; Food Analysis - methods ; Malonates - isolation & purification ; Malondialdehyde - isolation & purification ; Spectrophotometry ; Thiobarbiturates</subject><ispartof>Analytical biochemistry, 1983, Vol.128 (1), p.240-244</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-308ce7972afefd3b9dad848a0592d0a69ff606bd93a76a96bd2caf2f913245253</citedby><cites>FETCH-LOGICAL-c357t-308ce7972afefd3b9dad848a0592d0a69ff606bd93a76a96bd2caf2f913245253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269783903718$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6846796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bird, R.P.</creatorcontrib><creatorcontrib>Hung, Silas S.O.</creatorcontrib><creatorcontrib>Hadley, M.</creatorcontrib><creatorcontrib>Draper, H.H.</creatorcontrib><title>Determination of malonaldehyde in biological materials by high-pressure liquid chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a μBondapak C
18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1–2 μg of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.</description><subject>Animal Feed - analysis</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Food Analysis - methods</subject><subject>Malonates - isolation & purification</subject><subject>Malondialdehyde - isolation & purification</subject><subject>Spectrophotometry</subject><subject>Thiobarbiturates</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFFL5DAQx8Oh6LrnNzihT6IPPSdNmzYvgninHgi-6OMR0mSyjbTNmrTCfnuz7uKjTzPw_80M8yPkF4XfFCi_AgCWF1zUFw27FMBqmjc_yIKC4DkwEAdk8YUck5MYXwEoLSt-RI54U_Ja8AX5_wcnDIMb1eT8mHmbDar3o-oNdhuDmRuz1vner5xWfcoS7FQfs3aTdW7V5euAMc4Bs969zc5kugs-UX4V1Lrb_CSHNtF4uq9L8nL39_n2IX98uv93e_OYa1bVU86g0ViLulAWrWGtMMo0ZaOgEoUBxYW1HHhrBFM1VyJ1hVa2sIKyoqyKii3J-W7vOvi3GeMkBxc19r0a0c9RNlDWVdKQwHIH6uBjDGjlOrhBhY2kILdW5VaZ3CqTDZOfVlOzJGf7_XM7oPka2mtM-fUux_Tku8Mgo3Y4ajQuoJ6k8e77Ax8UyYiW</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Bird, R.P.</creator><creator>Hung, Silas S.O.</creator><creator>Hadley, M.</creator><creator>Draper, H.H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1983</creationdate><title>Determination of malonaldehyde in biological materials by high-pressure liquid chromatography</title><author>Bird, R.P. ; Hung, Silas S.O. ; Hadley, M. ; Draper, H.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-308ce7972afefd3b9dad848a0592d0a69ff606bd93a76a96bd2caf2f913245253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animal Feed - analysis</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Food Analysis - methods</topic><topic>Malonates - isolation & purification</topic><topic>Malondialdehyde - isolation & purification</topic><topic>Spectrophotometry</topic><topic>Thiobarbiturates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bird, R.P.</creatorcontrib><creatorcontrib>Hung, Silas S.O.</creatorcontrib><creatorcontrib>Hadley, M.</creatorcontrib><creatorcontrib>Draper, H.H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bird, R.P.</au><au>Hung, Silas S.O.</au><au>Hadley, M.</au><au>Draper, H.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of malonaldehyde in biological materials by high-pressure liquid chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1983</date><risdate>1983</risdate><volume>128</volume><issue>1</issue><spage>240</spage><epage>244</epage><pages>240-244</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a μBondapak C
18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1–2 μg of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6846796</pmid><doi>10.1016/0003-2697(83)90371-8</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1983, Vol.128 (1), p.240-244 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80475697 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal Feed - analysis Chromatography, High Pressure Liquid - methods Food Analysis - methods Malonates - isolation & purification Malondialdehyde - isolation & purification Spectrophotometry Thiobarbiturates |
| title | Determination of malonaldehyde in biological materials by high-pressure liquid chromatography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T23%3A41%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20malonaldehyde%20in%20biological%20materials%20by%20high-pressure%20liquid%20chromatography&rft.jtitle=Analytical%20biochemistry&rft.au=Bird,%20R.P.&rft.date=1983&rft.volume=128&rft.issue=1&rft.spage=240&rft.epage=244&rft.pages=240-244&rft.issn=0003-2697&rft.eissn=1096-0309&rft_id=info:doi/10.1016/0003-2697(83)90371-8&rft_dat=%3Cproquest_cross%3E80475697%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80475697&rft_id=info:pmid/6846796&rft_els_id=0003269783903718&rfr_iscdi=true |