Methods for mycelial breakage and isolation of mitochondria and vacuoles of neurospora
A new and inexpensive glass-bead blender allows rapid, easy, and controlled cell breakage of Neurospora, with good organellar survival. The yield of mitochondria and vacuoles is comparable to or better than methods involving sand grinding or snail-gut digestion of cell walls. A method for removing c...
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| Veröffentlicht in: | Analytical biochemistry 1983-02, Vol.128 (2), p.384-392 |
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| container_title | Analytical biochemistry |
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| creator | Cramer, Carole L. Ristow, Janet L. Paulus, Thomas J. Davis, Rowland H. |
| description | A new and inexpensive glass-bead blender allows rapid, easy, and controlled cell breakage of
Neurospora, with good organellar survival. The yield of mitochondria and vacuoles is comparable to or better than methods involving sand grinding or snail-gut digestion of cell walls. A method for removing cell wall fragments from a crude homogenate is described. Isolation of mitochondria and vacuoles from the crude homogenate with little cross-contamination is accomplished by density-gradient centrifugation in a fixed-angle rotor (Sorvall). |
| doi_str_mv | 10.1016/0003-2697(83)90390-1 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1983-02, Vol.128 (2), p.384-392 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80472618 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cell Fractionation - methods Cell Membrane Microscopy, Electron Mitochondria Neurospora Neurospora - ultrastructure Neurospora crassa - ultrastructure |
| title | Methods for mycelial breakage and isolation of mitochondria and vacuoles of neurospora |
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