The Ca2(+)-sensitive cytosolic phospholipase A2 is a 100-kDa protein in human monoblast U937 cells

Human monoblast U937 cells contain a soluble phospholipase A2 (PLA2) that is activated over the range of 150-600 nM Ca2+ and is stable only at neutral pH. We have purified this PLA2 over 34,000-fold to near homogeneity using sequential ion exchange, hydrophobic interaction, and gel filtration chroma...

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Veröffentlicht in:	The Journal of biological chemistry 1991-03, Vol.266 (8), p.5268-5272
Hauptverfasser:	Kramer, R M, Roberts, E F, Manetta, J, Putnam, J E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Blotting, Western Calcium - metabolism Cell Line Chromatography, Liquid Cytosol - enzymology Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Hydrolases Immunohistochemistry Kinetics Molecular Weight Phospholipases A - isolation & purification Phospholipases A - metabolism Phospholipases A2 Precipitin Tests
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