Chromatographic analysis of sugars in physiological fluids by postcolumn reaction with cuprammonium: A new and highly sensitive method

A new highly sensitive method of high-performance liquid chromatographic analysis has been used to separate mono-, di-, and oligosaccharides present in jejunal aspirates from experimentally perfused animals. The sugars in the column eluant are detected at 280 to 310 nm after reaction with cuprammoni...

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Veröffentlicht in:	Analytical biochemistry 1983-02, Vol.128 (2), p.422-428
Hauptverfasser:	Grimble, George K., Barker, Helen M., Taylor, Rodney H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia Animals Carbohydrates - analysis Carbohydrates - isolation & purification Chromatography, High Pressure Liquid - methods Copper Copper Sulfate Indicators and Reagents Intestine, Small - metabolism Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
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