Ascorbic acid and the binding of DA agonists to neostriatal membrane preparations
A considerable controversy exists over whether ascorbic acid should or should not be used in experiments in which the binding of DA agonists to neostriatal membrane preparations is being determined. Some authors claim that its presence is required. In the present study we have determined that sodium...
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| Veröffentlicht in: | Neuropharmacology 1983, Vol.22 (1), p.135-137 |
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| container_title | Neuropharmacology |
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| creator | Heikkila, R.E. Manzino, L. Cabbat, F.S. Gershefski Hanly, J. |
| description | A considerable controversy exists over whether ascorbic acid should or should not be used in experiments in which the binding of DA agonists to neostriatal membrane preparations is being determined. Some authors claim that its presence is required. In the present study we have determined that sodium ascorbate was a very potent inhibitor of the specific binding of
3H-DA to a rat neostriatal membrane preparation. Under conditions of these binding experiments, there was no decomposition of the
3H-DA as determined by two separate techniques. These data would seem to suggest that when
3H-DA binding is being measured, ascorbate addition is detrimental. |
| doi_str_mv | 10.1016/0028-3908(83)90274-5 |
| format | Article |
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3H-DA to a rat neostriatal membrane preparation. Under conditions of these binding experiments, there was no decomposition of the
3H-DA as determined by two separate techniques. These data would seem to suggest that when
3H-DA binding is being measured, ascorbate addition is detrimental.</description><identifier>ISSN: 0028-3908</identifier><identifier>EISSN: 1873-7064</identifier><identifier>DOI: 10.1016/0028-3908(83)90274-5</identifier><identifier>PMID: 6843783</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; ascorbic acid ; Ascorbic Acid - metabolism ; Cell Membrane - metabolism ; Corpus Striatum - ultrastructure ; dopamine ; Dopamine - metabolism ; rats ; striate cortex ; Tritium</subject><ispartof>Neuropharmacology, 1983, Vol.22 (1), p.135-137</ispartof><rights>1983 Pergamon Press Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-c0657f55000342afe883a92b6b658f5d75cee0746c7551ccb3d3134ee5a8320c3</citedby><cites>FETCH-LOGICAL-c388t-c0657f55000342afe883a92b6b658f5d75cee0746c7551ccb3d3134ee5a8320c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0028-3908(83)90274-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6843783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heikkila, R.E.</creatorcontrib><creatorcontrib>Manzino, L.</creatorcontrib><creatorcontrib>Cabbat, F.S.</creatorcontrib><creatorcontrib>Gershefski Hanly, J.</creatorcontrib><title>Ascorbic acid and the binding of DA agonists to neostriatal membrane preparations</title><title>Neuropharmacology</title><addtitle>Neuropharmacology</addtitle><description>A considerable controversy exists over whether ascorbic acid should or should not be used in experiments in which the binding of DA agonists to neostriatal membrane preparations is being determined. Some authors claim that its presence is required. In the present study we have determined that sodium ascorbate was a very potent inhibitor of the specific binding of
3H-DA to a rat neostriatal membrane preparation. Under conditions of these binding experiments, there was no decomposition of the
3H-DA as determined by two separate techniques. These data would seem to suggest that when
3H-DA binding is being measured, ascorbate addition is detrimental.</description><subject>Animals</subject><subject>ascorbic acid</subject><subject>Ascorbic Acid - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Corpus Striatum - ultrastructure</subject><subject>dopamine</subject><subject>Dopamine - metabolism</subject><subject>rats</subject><subject>striate cortex</subject><subject>Tritium</subject><issn>0028-3908</issn><issn>1873-7064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotVb_gUJWoovRZPLsRij1CQURdB0ymTs10pnUJBX8905t6VK5i7u455x7-BA6peSKEiqvCSl1wcZEX2h2OSal4oXYQ0OqFSsUkXwfDXeSQ3SU0gchhGuqB2ggNWdKsyF6mSQXYuUdts7X2HY1zu-AK9_Vvpvj0ODbCbbz0PmUE84BdxBSjt5mu8AttFW0HeBlhKWNNvvQpWN00NhFgpPtHqG3-7vX6WMxe354mk5mhWNa58IRKVQjRN-J8dI2oDWz47KSlRS6EbUSDoAoLp0SgjpXsZpRxgGE1awkjo3Q-SZ3GcPnClI2rU8OFou-UFglowmXggr1r5AKIZnoZ4T4RuhiSClCY5bRtzZ-G0rMGrlZ8zRrnkYz84vcrG1n2_xV1UK9M20Z9_ebzR16Gl8eoknOQ-eg9hFcNnXwfz_4AYzCjzk</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Heikkila, R.E.</creator><creator>Manzino, L.</creator><creator>Cabbat, F.S.</creator><creator>Gershefski Hanly, J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1983</creationdate><title>Ascorbic acid and the binding of DA agonists to neostriatal membrane preparations</title><author>Heikkila, R.E. ; Manzino, L. ; Cabbat, F.S. ; Gershefski Hanly, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-c0657f55000342afe883a92b6b658f5d75cee0746c7551ccb3d3134ee5a8320c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>ascorbic acid</topic><topic>Ascorbic Acid - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Corpus Striatum - ultrastructure</topic><topic>dopamine</topic><topic>Dopamine - metabolism</topic><topic>rats</topic><topic>striate cortex</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heikkila, R.E.</creatorcontrib><creatorcontrib>Manzino, L.</creatorcontrib><creatorcontrib>Cabbat, F.S.</creatorcontrib><creatorcontrib>Gershefski Hanly, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuropharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heikkila, R.E.</au><au>Manzino, L.</au><au>Cabbat, F.S.</au><au>Gershefski Hanly, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ascorbic acid and the binding of DA agonists to neostriatal membrane preparations</atitle><jtitle>Neuropharmacology</jtitle><addtitle>Neuropharmacology</addtitle><date>1983</date><risdate>1983</risdate><volume>22</volume><issue>1</issue><spage>135</spage><epage>137</epage><pages>135-137</pages><issn>0028-3908</issn><eissn>1873-7064</eissn><abstract>A considerable controversy exists over whether ascorbic acid should or should not be used in experiments in which the binding of DA agonists to neostriatal membrane preparations is being determined. Some authors claim that its presence is required. In the present study we have determined that sodium ascorbate was a very potent inhibitor of the specific binding of
3H-DA to a rat neostriatal membrane preparation. Under conditions of these binding experiments, there was no decomposition of the
3H-DA as determined by two separate techniques. These data would seem to suggest that when
3H-DA binding is being measured, ascorbate addition is detrimental.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6843783</pmid><doi>10.1016/0028-3908(83)90274-5</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-3908 |
| ispartof | Neuropharmacology, 1983, Vol.22 (1), p.135-137 |
| issn | 0028-3908 1873-7064 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals ascorbic acid Ascorbic Acid - metabolism Cell Membrane - metabolism Corpus Striatum - ultrastructure dopamine Dopamine - metabolism rats striate cortex Tritium |
| title | Ascorbic acid and the binding of DA agonists to neostriatal membrane preparations |
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