Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex
We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence betwe...
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| Veröffentlicht in: | FEBS letters 1991-02, Vol.279 (2), p.229-232 |
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| creator | Lau, Kim S. Lee, Jun Fisher, Charles W. Cox, Rody P. Chuang, David T. |
| description | We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type. |
| doi_str_mv | 10.1016/0014-5793(91)80155-V |
| format | Article |
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Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)80155-V</identifier><identifier>PMID: 2001734</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) ; Acyltransferases - genetics ; Alternative splicing ; Base Sequence ; Biological and medical sciences ; cDNA ; Cloning, Molecular ; DNA Mutational Analysis ; E2 gene ; Fundamental and applied biological sciences. Psychology ; Genes ; Genomic clone ; Humans ; Ketone Oxidoreductases - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Oligonucleotides - chemistry ; Polymerase Chain Reaction ; RNA Precursors - genetics ; RNA Splicing ; RNA, Messenger - genetics ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>FEBS letters, 1991-02, Vol.279 (2), p.229-232</ispartof><rights>1991</rights><rights>FEBS Letters 279 (1991) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527V-5f375b7c962b326aee55e6bc4cf5530e10ffbdb3e77110d2837253e6b32c71403</citedby><cites>FETCH-LOGICAL-c527V-5f375b7c962b326aee55e6bc4cf5530e10ffbdb3e77110d2837253e6b32c71403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939180155V$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19634125$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2001734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lau, Kim S.</creatorcontrib><creatorcontrib>Lee, Jun</creatorcontrib><creatorcontrib>Fisher, Charles W.</creatorcontrib><creatorcontrib>Cox, Rody P.</creatorcontrib><creatorcontrib>Chuang, David T.</creatorcontrib><title>Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.</description><subject>3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)</subject><subject>Acyltransferases - genetics</subject><subject>Alternative splicing</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>DNA Mutational Analysis</subject><subject>E2 gene</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genomic clone</subject><subject>Humans</subject><subject>Ketone Oxidoreductases - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Oligonucleotides - chemistry</subject><subject>Polymerase Chain Reaction</subject><subject>RNA Precursors - genetics</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Genes</topic><topic>Genomic clone</topic><topic>Humans</topic><topic>Ketone Oxidoreductases - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - genetics</topic><topic>Oligonucleotides - chemistry</topic><topic>Polymerase Chain Reaction</topic><topic>RNA Precursors - genetics</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lau, Kim S.</creatorcontrib><creatorcontrib>Lee, Jun</creatorcontrib><creatorcontrib>Fisher, Charles W.</creatorcontrib><creatorcontrib>Cox, Rody P.</creatorcontrib><creatorcontrib>Chuang, David T.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lau, Kim S.</au><au>Lee, Jun</au><au>Fisher, Charles W.</au><au>Cox, Rody P.</au><au>Chuang, David T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-02-25</date><risdate>1991</risdate><volume>279</volume><issue>2</issue><spage>229</spage><epage>232</epage><pages>229-232</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>We have isolated a human genomic clone hgE2-14 containing exons 5,6,7 and 8 of the branched-chain α-ketoacid dehydrogenase E2 transacylase gene. Sequencing of exon B and its surrounding intronic sequences reveals complete identity with the previously reported truncated E2 cDNA (hE2-1) sequence between nucleotides 938 and 1521. We have identified consensus splice site junctions flanking exon 8 and also a cryptic 3??? splice site 370 bases upstream from the start of exon 8 in the gene. In addition, two polyadenylation signals located in the hE2-1 cDNA are also present in the intronic sequence downstream of exon 8 which promote termination of transcription. The data indicate that shortened human liver E2 transcripts undergo alternative splicing to yield mRNA of the hE2-1 type.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2001734</pmid><doi>10.1016/0014-5793(91)80155-V</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1991-02, Vol.279 (2), p.229-232 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Acyltransferases - genetics Alternative splicing Base Sequence Biological and medical sciences cDNA Cloning, Molecular DNA Mutational Analysis E2 gene Fundamental and applied biological sciences. Psychology Genes Genomic clone Humans Ketone Oxidoreductases - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Multienzyme Complexes - genetics Oligonucleotides - chemistry Polymerase Chain Reaction RNA Precursors - genetics RNA Splicing RNA, Messenger - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | Premature termination of transcription and alternative splicing in the human transacylase (E2) gene of the branched-chain α-ketoacid dehydrogenase complex |
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