Amplification of ssb-1 mutant single-stranded DNA-binding protein in Escherichia coli

The ssb-1 gene encoding a mutant Escherichia coli single-stranded DNA-binding protein has been cloned into plasmid pACYC184. The amount of overproduction of the cloned ssb-1 gene is dependent upon its orientation in the plasmid. In the less efficient orientation, 25-fold more mutant protein is produ...

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Veröffentlicht in:	Journal of molecular biology 1983-01, Vol.164 (2), p.193-211
Hauptverfasser:	Chase, John W., Murphy, Janet B., Whittier, Robert F., Lorensen, Elke, Sninsky, John J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - biosynthesis Carrier Proteins - biosynthesis Carrier Proteins - genetics Chromosomes, Bacterial Cloning, Molecular Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli - radiation effects Gene Amplification Genes, Bacterial Mutation Plasmids Rec A Recombinases Ultraviolet Rays
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