Human interleukin 1β fused to the human growth hormone signal peptide is N-glycosylated and secreted by Chinese hamster ovary cells

A hybrid gene consisting of the sequences coding for the signal peptide of human growth hormone and the mature form of interleukin-1β (IL-1β) was chemically synthesized. This sequence was inserted into a eukaryotic expression vector and introduced into Chinese hamster ovary cells. The resulting stab...

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Veröffentlicht in:	Gene 1991-01, Vol.97 (2), p.253-258
Hauptverfasser:	Pecceu, F., Dousset, P., Shire, D., Cavrois, E., Marchese, E., Ferrara, P., Kaghad, M., Dumont, X., Lupker, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Biotechnology Cell Line Cloning, Molecular Cricetinae eukaryotic expression vectors Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Glycosylation Growth Hormone - genetics Humans Interleukin-1 - genetics Interleukin-1 - metabolism Methods. Procedures. Technologies Molecular Sequence Data Precipitin Tests Protein Sorting Signals - genetics Recombinant DNA Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism rerouting synthetic gene Tunicamycin - pharmacology
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