A morphological and phenotypic analysis of Walker 256 cells
A detailed morphological analysis of Walker 256 cells sensitive and resistant to cis-diamminedichloroplatinum(II) has been performed. Two cell populations are identified by electron microscopy of differing differentiation corresponding structurally to cells reported in experimentally induced metasta...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1991-02, Vol.51 (4), p.1334-1338 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | SIMPKINS, H LEHMAN, J. M MAZURKIEWICZ, J. E DAVIS, B. H |
| description | A detailed morphological analysis of Walker 256 cells sensitive and resistant to cis-diamminedichloroplatinum(II) has been performed. Two cell populations are identified by electron microscopy of differing differentiation corresponding structurally to cells reported in experimentally induced metastases. Phenotyping of the cells using a number of monoclonal antibodies by immunocytochemistry and flow cytometry showed the absence of epithelial cell markers: however, the cells stained intensely for markers for germ and/or hematopoietic cells. Further studies utilizing monoclonal antibodies to lymphoid, myeloid, and monocytoid cells showed the cells to be monocytoid in origin. No evidence of cell heterogeneity was evident from the phenotypic experiments (a biphasic pattern was not observed). Enzyme histochemistry showed strong focal acid phosphatase activity suggestive of cells of hematopoietic origin. Thus the concept that these cells reflect an epithelial cell of origin is not substantiated by phenotyping with two methodologies. |
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M ; MAZURKIEWICZ, J. E ; DAVIS, B. H</creator><creatorcontrib>SIMPKINS, H ; LEHMAN, J. M ; MAZURKIEWICZ, J. E ; DAVIS, B. H</creatorcontrib><description>A detailed morphological analysis of Walker 256 cells sensitive and resistant to cis-diamminedichloroplatinum(II) has been performed. Two cell populations are identified by electron microscopy of differing differentiation corresponding structurally to cells reported in experimentally induced metastases. Phenotyping of the cells using a number of monoclonal antibodies by immunocytochemistry and flow cytometry showed the absence of epithelial cell markers: however, the cells stained intensely for markers for germ and/or hematopoietic cells. Further studies utilizing monoclonal antibodies to lymphoid, myeloid, and monocytoid cells showed the cells to be monocytoid in origin. No evidence of cell heterogeneity was evident from the phenotypic experiments (a biphasic pattern was not observed). Enzyme histochemistry showed strong focal acid phosphatase activity suggestive of cells of hematopoietic origin. Thus the concept that these cells reflect an epithelial cell of origin is not substantiated by phenotyping with two methodologies.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 1997171</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animal tumors. 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M</creatorcontrib><creatorcontrib>MAZURKIEWICZ, J. E</creatorcontrib><creatorcontrib>DAVIS, B. H</creatorcontrib><title>A morphological and phenotypic analysis of Walker 256 cells</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>A detailed morphological analysis of Walker 256 cells sensitive and resistant to cis-diamminedichloroplatinum(II) has been performed. Two cell populations are identified by electron microscopy of differing differentiation corresponding structurally to cells reported in experimentally induced metastases. Phenotyping of the cells using a number of monoclonal antibodies by immunocytochemistry and flow cytometry showed the absence of epithelial cell markers: however, the cells stained intensely for markers for germ and/or hematopoietic cells. Further studies utilizing monoclonal antibodies to lymphoid, myeloid, and monocytoid cells showed the cells to be monocytoid in origin. No evidence of cell heterogeneity was evident from the phenotypic experiments (a biphasic pattern was not observed). Enzyme histochemistry showed strong focal acid phosphatase activity suggestive of cells of hematopoietic origin. Thus the concept that these cells reflect an epithelial cell of origin is not substantiated by phenotyping with two methodologies.</description><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinoma 256, Walker - pathology</subject><subject>Cell Line</subject><subject>Cisplatin - pharmacology</subject><subject>DNA - analysis</subject><subject>Drug Resistance - genetics</subject><subject>Flow Cytometry</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Phenotype</subject><subject>Rats</subject><subject>Spontaneous animal tumors</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNj01LxDAYhIMo67r6E4Rc9FZ4kzdfxdOy-AULXhSPJc2mtpo2tWkP---t2IOnYZiHYeaErJlEk2kh5ClZA4DJpND8nFyk9DlbyUCuyIrluWaarcndlrZx6OsY4kfjbKC2O9C-9l0cj33jZmvDMTWJxoq-2_DlB8qlos6HkC7JWWVD8leLbsjbw_3r7inbvzw-77b7rOYqHzM03gnAErjLESQcELxRWhj0inPUnBkDKJl0ylRGeA3KWAdljiWrSudwQ27_evshfk8-jUXbpN8FtvNxSoUBIRViPoPXCziVrT8U_dC0djgWy9s5v1lym-av1WA716R_mALGGccfcgVb4Q</recordid><startdate>19910215</startdate><enddate>19910215</enddate><creator>SIMPKINS, H</creator><creator>LEHMAN, J. 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Experimental tumors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinoma 256, Walker - pathology</topic><topic>Cell Line</topic><topic>Cisplatin - pharmacology</topic><topic>DNA - analysis</topic><topic>Drug Resistance - genetics</topic><topic>Flow Cytometry</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Phenotype</topic><topic>Rats</topic><topic>Spontaneous animal tumors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SIMPKINS, H</creatorcontrib><creatorcontrib>LEHMAN, J. M</creatorcontrib><creatorcontrib>MAZURKIEWICZ, J. E</creatorcontrib><creatorcontrib>DAVIS, B. 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Further studies utilizing monoclonal antibodies to lymphoid, myeloid, and monocytoid cells showed the cells to be monocytoid in origin. No evidence of cell heterogeneity was evident from the phenotypic experiments (a biphasic pattern was not observed). Enzyme histochemistry showed strong focal acid phosphatase activity suggestive of cells of hematopoietic origin. Thus the concept that these cells reflect an epithelial cell of origin is not substantiated by phenotyping with two methodologies.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>1997171</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1991-02, Vol.51 (4), p.1334-1338 |
| issn | 0008-5472 1538-7445 |
| language | eng |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animal tumors. Experimental tumors Animals Biological and medical sciences Carcinoma 256, Walker - pathology Cell Line Cisplatin - pharmacology DNA - analysis Drug Resistance - genetics Flow Cytometry Immunohistochemistry Medical sciences Microscopy, Electron Phenotype Rats Spontaneous animal tumors Tumors |
| title | A morphological and phenotypic analysis of Walker 256 cells |
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