Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on β-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH
The chain elongation products from [2- 14C]malonyl-CoA and palmitoyl-CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti-NADPH-cytochrome P450 reductase or anti-cytochrome b 5 immunoglobulin G were included in this system, not only was the overall chain el...
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| Veröffentlicht in: | FEBS letters 1983-05, Vol.155 (1), p.11-14 |
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| creator | Nagao, Masaki Ishibashi, Teruo Okayasu, Takako Imai, Yoh |
| description | The chain elongation products from [2-
14C]malonyl-CoA and palmitoyl-CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti-NADPH-cytochrome P450 reductase or anti-cytochrome
b
5 immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2-heptadecanone derived from an intermediate β-ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH-cytochrome P450 reductase and cytochrome
b
5 participate in the conversion from β-ketostearoyl-CoA to β-hydroxystearoyl-CoA, which is the first reductive step of the microsomal chain elongating system. |
| doi_str_mv | 10.1016/0014-5793(83)80198-7 |
| format | Article |
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b
5 immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2-heptadecanone derived from an intermediate β-ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH-cytochrome P450 reductase and cytochrome
b
5 participate in the conversion from β-ketostearoyl-CoA to β-hydroxystearoyl-CoA, which is the first reductive step of the microsomal chain elongating system.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(83)80198-7</identifier><identifier>PMID: 6404652</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Acyl Coenzyme A - metabolism ; Animals ; Chain elongation ; Chromatography, Gas ; Cytochrome b Group - metabolism ; Cytochrome b5 ; Cytochromes b5 ; F pT ; Fatty acid ; Fatty Acids - metabolism ; Immunochemical evidence ; Microsome ; Microsomes, Liver - metabolism ; NADP - metabolism ; NADPH-Ferrihemoprotein Reductase - metabolism ; Rabbits ; Rats</subject><ispartof>FEBS letters, 1983-05, Vol.155 (1), p.11-14</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(83)80198-7$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6404652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagao, Masaki</creatorcontrib><creatorcontrib>Ishibashi, Teruo</creatorcontrib><creatorcontrib>Okayasu, Takako</creatorcontrib><creatorcontrib>Imai, Yoh</creatorcontrib><title>Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on β-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The chain elongation products from [2-
14C]malonyl-CoA and palmitoyl-CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti-NADPH-cytochrome P450 reductase or anti-cytochrome
b
5 immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2-heptadecanone derived from an intermediate β-ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH-cytochrome P450 reductase and cytochrome
b
5 participate in the conversion from β-ketostearoyl-CoA to β-hydroxystearoyl-CoA, which is the first reductive step of the microsomal chain elongating system.</description><subject>Acyl Coenzyme A - metabolism</subject><subject>Animals</subject><subject>Chain elongation</subject><subject>Chromatography, Gas</subject><subject>Cytochrome b Group - metabolism</subject><subject>Cytochrome b5</subject><subject>Cytochromes b5</subject><subject>F pT</subject><subject>Fatty acid</subject><subject>Fatty Acids - metabolism</subject><subject>Immunochemical evidence</subject><subject>Microsome</subject><subject>Microsomes, Liver - metabolism</subject><subject>NADP - metabolism</subject><subject>NADPH-Ferrihemoprotein Reductase - metabolism</subject><subject>Rabbits</subject><subject>Rats</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc-KFDEQxoMo67j6Bgo5yXrImnSS7vRFGMY_u7Csc9BzSNLVbrS7MybpgX6ivfsgPpOZnWERCori91XxUR9Crxm9ZJTV7yllgsim5ReKv1OUtYo0T9CKqYYTLmr1FK0eJc_Ri5R-0jIr1p6hs1pQUctqhe63ISVvB8B-2odhDyNMGYce364_bq-IW3JwdzGMgLdCUhyhm102CbCZOvwftRKHCf_9Q35BDimDiWEZyCasTyu-UD_h0bsYUhjNgHuT84KN8-XOnSkMhjD9MA_KNO92IWbosF2OTl6iZ70ZErw69XP0_fOnb5srcvP1y_VmfUOA0yYT7mpprQLGetkpsLRqWdsxCU0nlWyqmtkKlBKyUuVPva16rqSthWpbq7gz_By9Pd7dxfB7hpT16JODYTAThDlpRYUUUooifHMSznaETu-iH01c9OmzhX84cihu9x6iTs7D5KDzEVzWXfCaUX1IUh9i0oeYtCp1SFI3_B9koJGc</recordid><startdate>19830502</startdate><enddate>19830502</enddate><creator>Nagao, Masaki</creator><creator>Ishibashi, Teruo</creator><creator>Okayasu, Takako</creator><creator>Imai, Yoh</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19830502</creationdate><title>Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on β-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH</title><author>Nagao, Masaki ; Ishibashi, Teruo ; Okayasu, Takako ; Imai, Yoh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e307t-3c65bb8e11f5d8eb02919d15e7d5857261b2e884528187fb2f385b64899b83ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Acyl Coenzyme A - metabolism</topic><topic>Animals</topic><topic>Chain elongation</topic><topic>Chromatography, Gas</topic><topic>Cytochrome b Group - metabolism</topic><topic>Cytochrome b5</topic><topic>Cytochromes b5</topic><topic>F pT</topic><topic>Fatty acid</topic><topic>Fatty Acids - metabolism</topic><topic>Immunochemical evidence</topic><topic>Microsome</topic><topic>Microsomes, Liver - metabolism</topic><topic>NADP - metabolism</topic><topic>NADPH-Ferrihemoprotein Reductase - metabolism</topic><topic>Rabbits</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagao, Masaki</creatorcontrib><creatorcontrib>Ishibashi, Teruo</creatorcontrib><creatorcontrib>Okayasu, Takako</creatorcontrib><creatorcontrib>Imai, Yoh</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagao, Masaki</au><au>Ishibashi, Teruo</au><au>Okayasu, Takako</au><au>Imai, Yoh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on β-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1983-05-02</date><risdate>1983</risdate><volume>155</volume><issue>1</issue><spage>11</spage><epage>14</epage><pages>11-14</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The chain elongation products from [2-
14C]malonyl-CoA and palmitoyl-CoA by rat liver microsomes initiated by NADPH were found mainly to be 18:1 and 18:0. When anti-NADPH-cytochrome P450 reductase or anti-cytochrome
b
5 immunoglobulin G were included in this system, not only was the overall chain elongation significantly suppressed, but also a new radioactive product, which was identified as 2-heptadecanone derived from an intermediate β-ketostearate, is accumulated depending upon the amounts of IgG added. These results suggest that NADPH-cytochrome P450 reductase and cytochrome
b
5 participate in the conversion from β-ketostearoyl-CoA to β-hydroxystearoyl-CoA, which is the first reductive step of the microsomal chain elongating system.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>6404652</pmid><doi>10.1016/0014-5793(83)80198-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1983-05, Vol.155 (1), p.11-14 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80454554 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acyl Coenzyme A - metabolism Animals Chain elongation Chromatography, Gas Cytochrome b Group - metabolism Cytochrome b5 Cytochromes b5 F pT Fatty acid Fatty Acids - metabolism Immunochemical evidence Microsome Microsomes, Liver - metabolism NADP - metabolism NADPH-Ferrihemoprotein Reductase - metabolism Rabbits Rats |
| title | Possible involvement of NADPH-cytochrome P450 reductase and cytochrome b5 on β-ketostearoyl-CoA reduction in microsomal fatty acid chain elongation supported by NADPH |
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