Acid Phosphatase (EC 3.1.3.2) Activity in Alveolar Macrophages from Patients with Active Sarcoidosis
Five main acid phosphatase (AcP) zones have been recognized and studied by polyacrylamide-gel electrophoresis. Band 5 represents the only tartrate-resistant form and is present in bone osteoclasts and in human alveolar macrophages (AMs). This study was carried out to quantify the presence of total a...
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| Veröffentlicht in: | Chest 1991-03, Vol.99 (3), p.546-550 |
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| creator | Capelli, Armando Lusuardi, Mirco Carli, Sonia Donner, Claudio F. |
| description | Five main acid phosphatase (AcP) zones have been recognized and studied by polyacrylamide-gel electrophoresis. Band 5 represents the only tartrate-resistant form and is present in bone osteoclasts and in human alveolar macrophages (AMs). This study was carried out to quantify the presence of total and tartrate-resistant AcP (TrAcP) in AMs from bronchoalveolar lavage (BAL) of 11 patients with first stage sarcoidosis and in 13 nonsmokers and 16 smokers serving as control healthy subjects. The AMs from smokers showed an increase in total AcP activity (115.9 ± 77.8 mU/106); on the contrary, macrophages of patients with sarcoidosis revealed a consistent decrease in total AcP (27.8 ± 7.0 mU/106) and particularly the TrAcP subtype (14.8 ± 3.7 mU/106) in comparison with control nonsmokers (AcP = 42.2 ± 18.9 mU/106 [p = NS]; TrAcP = 35.1 ± 15.1 mU/106 [p |
| doi_str_mv | 10.1378/chest.99.3.546 |
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Band 5 represents the only tartrate-resistant form and is present in bone osteoclasts and in human alveolar macrophages (AMs). This study was carried out to quantify the presence of total and tartrate-resistant AcP (TrAcP) in AMs from bronchoalveolar lavage (BAL) of 11 patients with first stage sarcoidosis and in 13 nonsmokers and 16 smokers serving as control healthy subjects. The AMs from smokers showed an increase in total AcP activity (115.9 ± 77.8 mU/106); on the contrary, macrophages of patients with sarcoidosis revealed a consistent decrease in total AcP (27.8 ± 7.0 mU/106) and particularly the TrAcP subtype (14.8 ± 3.7 mU/106) in comparison with control nonsmokers (AcP = 42.2 ± 18.9 mU/106 [p = NS]; TrAcP = 35.1 ± 15.1 mU/106 [p<0.005]). The decrease in TrAcP activity was inversely correlated with the lymphocyte number (r = −0.75; p<0.01), lymphocyte percentage (r = −0.62; p<0.05), and CD4/CD8 ratio (r = −0.61; p<0.05). After six months of follow-up, the cytologic BAL picture returned completely to normal in five patients with full spontaneous regression of sarcoidosis; and also at the same time, normal values of TrAcP activity were restored. Since TrAcP activity can be easily detected, its possible use, along with the lymphocyte count and CD4/CD8 ratio, as a prognostic indicator of the clinical course of sarcoidosis deserves further investigation. (Chest 1991; 99:545–50)</description><identifier>ISSN: 0012-3692</identifier><identifier>EISSN: 1931-3543</identifier><identifier>DOI: 10.1378/chest.99.3.546</identifier><identifier>PMID: 1671655</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acid Phosphatase - metabolism ; AcP = acid phosphatase ; Adult ; AM = alveolar macrophage ; Bronchoalveolar Lavage Fluid - pathology ; CD4-Positive T-Lymphocytes - pathology ; Female ; Histocytochemistry ; Humans ; Leukocyte Count ; Lung Diseases - enzymology ; Lung Diseases - pathology ; Macrophages - enzymology ; Male ; Pulmonary Alveoli - pathology ; Regression Analysis ; Sarcoidosis - enzymology ; Sarcoidosis - pathology ; Smoking - metabolism ; Smoking - pathology ; T-Lymphocytes, Regulatory - pathology ; Tartrates ; TrAcP = tartrate-resistant acid phosphatase ; TsAcP = tartrate-sensitive acid phosphatase</subject><ispartof>Chest, 1991-03, Vol.99 (3), p.546-550</ispartof><rights>1991 The American College of Chest Physicians</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-2b896e4e373d6153eaf0c56e07d922a4737fc9bf1d10cd68b5cee3e46e13740c3</citedby><cites>FETCH-LOGICAL-c409t-2b896e4e373d6153eaf0c56e07d922a4737fc9bf1d10cd68b5cee3e46e13740c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1671655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Capelli, Armando</creatorcontrib><creatorcontrib>Lusuardi, Mirco</creatorcontrib><creatorcontrib>Carli, Sonia</creatorcontrib><creatorcontrib>Donner, Claudio F.</creatorcontrib><title>Acid Phosphatase (EC 3.1.3.2) Activity in Alveolar Macrophages from Patients with Active Sarcoidosis</title><title>Chest</title><addtitle>Chest</addtitle><description>Five main acid phosphatase (AcP) zones have been recognized and studied by polyacrylamide-gel electrophoresis. Band 5 represents the only tartrate-resistant form and is present in bone osteoclasts and in human alveolar macrophages (AMs). This study was carried out to quantify the presence of total and tartrate-resistant AcP (TrAcP) in AMs from bronchoalveolar lavage (BAL) of 11 patients with first stage sarcoidosis and in 13 nonsmokers and 16 smokers serving as control healthy subjects. The AMs from smokers showed an increase in total AcP activity (115.9 ± 77.8 mU/106); on the contrary, macrophages of patients with sarcoidosis revealed a consistent decrease in total AcP (27.8 ± 7.0 mU/106) and particularly the TrAcP subtype (14.8 ± 3.7 mU/106) in comparison with control nonsmokers (AcP = 42.2 ± 18.9 mU/106 [p = NS]; TrAcP = 35.1 ± 15.1 mU/106 [p<0.005]). The decrease in TrAcP activity was inversely correlated with the lymphocyte number (r = −0.75; p<0.01), lymphocyte percentage (r = −0.62; p<0.05), and CD4/CD8 ratio (r = −0.61; p<0.05). After six months of follow-up, the cytologic BAL picture returned completely to normal in five patients with full spontaneous regression of sarcoidosis; and also at the same time, normal values of TrAcP activity were restored. Since TrAcP activity can be easily detected, its possible use, along with the lymphocyte count and CD4/CD8 ratio, as a prognostic indicator of the clinical course of sarcoidosis deserves further investigation. (Chest 1991; 99:545–50)</description><subject>Acid Phosphatase - metabolism</subject><subject>AcP = acid phosphatase</subject><subject>Adult</subject><subject>AM = alveolar macrophage</subject><subject>Bronchoalveolar Lavage Fluid - pathology</subject><subject>CD4-Positive T-Lymphocytes - pathology</subject><subject>Female</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Leukocyte Count</subject><subject>Lung Diseases - enzymology</subject><subject>Lung Diseases - pathology</subject><subject>Macrophages - enzymology</subject><subject>Male</subject><subject>Pulmonary Alveoli - pathology</subject><subject>Regression Analysis</subject><subject>Sarcoidosis - enzymology</subject><subject>Sarcoidosis - pathology</subject><subject>Smoking - metabolism</subject><subject>Smoking - pathology</subject><subject>T-Lymphocytes, Regulatory - pathology</subject><subject>Tartrates</subject><subject>TrAcP = tartrate-resistant acid phosphatase</subject><subject>TsAcP = tartrate-sensitive acid phosphatase</subject><issn>0012-3692</issn><issn>1931-3543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9PHCEcR4mp0VV79daEU6OHmcLAMHDcbOyPxEYT7Zmw8B0HM7OswK7xvy91TOyl6YmQvM8LPITOKakp6-QXO0DKtVI1q1suDtCCKkYr1nL2AS0IoU3FhGqO0UlKj6TcqRJH6IiKjoq2XSC3tN7h2yGk7WCySYAvrlaY1bT4mku8tNnvfX7BfoOX4x7CaCL-aWwMBX-AhPsYJnxrsodNTvjZ52HeAL4z0QbvQvLpDB32Zkzw8e08Rb--Xt2vvlfXN99-rJbXleVE5apZSyWAA-uYE7RlYHpiWwGkc6ppDO9Y11u17qmjxDoh160FYMAFlBKcWHaKPs_ebQxPu9JFTz5ZGEezgbBLWhLOO9mK_4JUUCqlJAWsZ7D8OKUIvd5GP5n4oinRf_rr1_5aKc106V8Gn97Mu_UE7h2fg78LB_8wPPsIOk1mHAvNZtVj2MWNGf8SynkAJdzeQ9TJltgWXBnbrF3w_3rLb0erpOA</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Capelli, Armando</creator><creator>Lusuardi, Mirco</creator><creator>Carli, Sonia</creator><creator>Donner, Claudio F.</creator><general>Elsevier Inc</general><general>American College of Chest Physicians</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910301</creationdate><title>Acid Phosphatase (EC 3.1.3.2) Activity in Alveolar Macrophages from Patients with Active Sarcoidosis</title><author>Capelli, Armando ; Lusuardi, Mirco ; Carli, Sonia ; Donner, Claudio F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-2b896e4e373d6153eaf0c56e07d922a4737fc9bf1d10cd68b5cee3e46e13740c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>AcP = acid phosphatase</topic><topic>Adult</topic><topic>AM = alveolar macrophage</topic><topic>Bronchoalveolar Lavage Fluid - pathology</topic><topic>CD4-Positive T-Lymphocytes - pathology</topic><topic>Female</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Leukocyte Count</topic><topic>Lung Diseases - enzymology</topic><topic>Lung Diseases - pathology</topic><topic>Macrophages - enzymology</topic><topic>Male</topic><topic>Pulmonary Alveoli - pathology</topic><topic>Regression Analysis</topic><topic>Sarcoidosis - enzymology</topic><topic>Sarcoidosis - pathology</topic><topic>Smoking - metabolism</topic><topic>Smoking - pathology</topic><topic>T-Lymphocytes, Regulatory - pathology</topic><topic>Tartrates</topic><topic>TrAcP = tartrate-resistant acid phosphatase</topic><topic>TsAcP = tartrate-sensitive acid phosphatase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capelli, Armando</creatorcontrib><creatorcontrib>Lusuardi, Mirco</creatorcontrib><creatorcontrib>Carli, Sonia</creatorcontrib><creatorcontrib>Donner, Claudio F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chest</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capelli, Armando</au><au>Lusuardi, Mirco</au><au>Carli, Sonia</au><au>Donner, Claudio F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acid Phosphatase (EC 3.1.3.2) Activity in Alveolar Macrophages from Patients with Active Sarcoidosis</atitle><jtitle>Chest</jtitle><addtitle>Chest</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>99</volume><issue>3</issue><spage>546</spage><epage>550</epage><pages>546-550</pages><issn>0012-3692</issn><eissn>1931-3543</eissn><abstract>Five main acid phosphatase (AcP) zones have been recognized and studied by polyacrylamide-gel electrophoresis. Band 5 represents the only tartrate-resistant form and is present in bone osteoclasts and in human alveolar macrophages (AMs). This study was carried out to quantify the presence of total and tartrate-resistant AcP (TrAcP) in AMs from bronchoalveolar lavage (BAL) of 11 patients with first stage sarcoidosis and in 13 nonsmokers and 16 smokers serving as control healthy subjects. The AMs from smokers showed an increase in total AcP activity (115.9 ± 77.8 mU/106); on the contrary, macrophages of patients with sarcoidosis revealed a consistent decrease in total AcP (27.8 ± 7.0 mU/106) and particularly the TrAcP subtype (14.8 ± 3.7 mU/106) in comparison with control nonsmokers (AcP = 42.2 ± 18.9 mU/106 [p = NS]; TrAcP = 35.1 ± 15.1 mU/106 [p<0.005]). The decrease in TrAcP activity was inversely correlated with the lymphocyte number (r = −0.75; p<0.01), lymphocyte percentage (r = −0.62; p<0.05), and CD4/CD8 ratio (r = −0.61; p<0.05). After six months of follow-up, the cytologic BAL picture returned completely to normal in five patients with full spontaneous regression of sarcoidosis; and also at the same time, normal values of TrAcP activity were restored. Since TrAcP activity can be easily detected, its possible use, along with the lymphocyte count and CD4/CD8 ratio, as a prognostic indicator of the clinical course of sarcoidosis deserves further investigation. (Chest 1991; 99:545–50)</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1671655</pmid><doi>10.1378/chest.99.3.546</doi><tpages>5</tpages></addata></record> |
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| ispartof | Chest, 1991-03, Vol.99 (3), p.546-550 |
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| subjects | Acid Phosphatase - metabolism AcP = acid phosphatase Adult AM = alveolar macrophage Bronchoalveolar Lavage Fluid - pathology CD4-Positive T-Lymphocytes - pathology Female Histocytochemistry Humans Leukocyte Count Lung Diseases - enzymology Lung Diseases - pathology Macrophages - enzymology Male Pulmonary Alveoli - pathology Regression Analysis Sarcoidosis - enzymology Sarcoidosis - pathology Smoking - metabolism Smoking - pathology T-Lymphocytes, Regulatory - pathology Tartrates TrAcP = tartrate-resistant acid phosphatase TsAcP = tartrate-sensitive acid phosphatase |
| title | Acid Phosphatase (EC 3.1.3.2) Activity in Alveolar Macrophages from Patients with Active Sarcoidosis |
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