Isolation of replication-competent molecular clones of visna virus
Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucle...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1991-03, Vol.181 (1), p.228-240 |
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| creator | Staskus, Katherine A. Retzel, Ernest F. Lewis, Elizabeth D. Silsby, Janet L. Sheila Cyr, S.T. Rank, Jeffrey M. Wietgrefe, Steven W. Haase, Ashley T. Cook, Ronald Fast, David Geiser, Paul T. Harty, John T. Kong, Selene H. Lahti, Carol J. Neufeld, Thomas P. Porter, Thomas E. Shoop, Elizabeth Zachow, Karen R. |
| description | Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection. |
| doi_str_mv | 10.1016/0042-6822(91)90488-W |
| format | Article |
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As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(91)90488-W</identifier><identifier>PMID: 1847257</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; ANTIGENE ; ANTIGENOS ; Base Sequence ; Biological and medical sciences ; Cells, Cultured ; Choroid Plexus ; Chromosome Deletion ; CLONE ; CLONES ; Cloning, Molecular ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; DESCENDANCE ; DNA Transposable Elements ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; FENOTIPOS ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Genetics ; Microbiology ; Molecular Sequence Data ; NUCLEOTIDE ; NUCLEOTIDOS ; Open Reading Frames ; OVIN ; OVINOS ; PHENOTYPE ; PROGENIE ; Sheep ; VARIACION GENETICA ; VARIATION GENETIQUE ; Viral Plaque Assay ; Virology ; Virus Replication ; VIRUS VISNA MAEDI ; Visna-maedi virus - genetics ; Visna-maedi virus - physiology</subject><ispartof>Virology (New York, N.Y.), 1991-03, Vol.181 (1), p.228-240</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-b3dee46df60371cc1e063c0afcd47d77f03d36bf9aa888f8ee20d0e2b4e1cba23</citedby><cites>FETCH-LOGICAL-c503t-b3dee46df60371cc1e063c0afcd47d77f03d36bf9aa888f8ee20d0e2b4e1cba23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/004268229190488W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19737298$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1847257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Staskus, Katherine A.</creatorcontrib><creatorcontrib>Retzel, Ernest F.</creatorcontrib><creatorcontrib>Lewis, Elizabeth D.</creatorcontrib><creatorcontrib>Silsby, Janet L.</creatorcontrib><creatorcontrib>Sheila Cyr, S.T.</creatorcontrib><creatorcontrib>Rank, Jeffrey M.</creatorcontrib><creatorcontrib>Wietgrefe, Steven W.</creatorcontrib><creatorcontrib>Haase, Ashley T.</creatorcontrib><creatorcontrib>Cook, Ronald</creatorcontrib><creatorcontrib>Fast, David</creatorcontrib><creatorcontrib>Geiser, Paul T.</creatorcontrib><creatorcontrib>Harty, John T.</creatorcontrib><creatorcontrib>Kong, Selene H.</creatorcontrib><creatorcontrib>Lahti, Carol J.</creatorcontrib><creatorcontrib>Neufeld, Thomas P.</creatorcontrib><creatorcontrib>Porter, Thomas E.</creatorcontrib><creatorcontrib>Shoop, Elizabeth</creatorcontrib><creatorcontrib>Zachow, Karen R.</creatorcontrib><title>Isolation of replication-competent molecular clones of visna virus</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Choroid Plexus</subject><subject>Chromosome Deletion</subject><subject>CLONE</subject><subject>CLONES</subject><subject>Cloning, Molecular</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>DESCENDANCE</subject><subject>DNA Transposable Elements</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>FENOTIPOS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>Open Reading Frames</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>PHENOTYPE</subject><subject>PROGENIE</subject><subject>Sheep</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><subject>Viral Plaque Assay</subject><subject>Virology</subject><subject>Virus Replication</subject><subject>VIRUS VISNA MAEDI</subject><subject>Visna-maedi virus - genetics</subject><subject>Visna-maedi virus - physiology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo6zj6B0RhLooeWisfk6Qvgi5-LCx40GWPIZ1UJJLujEn3gv_ezPbg3vSSULxPFVUPIc8ovKFA5VsAwTqpGXvV09c9CK2763tkQ6GXHXBB75PNX-QheVTrT2i1UnBGzqgWiu3Vhny4qDnZOeZpl8Ou4CFFd1t2Lo8HnHGad2NO6JZky86lPGE9kjexTra9ZamPyYNgU8Unp39Lrj59_H7-pbv8-vni_P1l5_bA527gHlFIHyRwRZ2jCJI7sMF5obxSAbjncgi9tVrroBEZeEA2CKRusIxvyct17qHkXwvW2YyxOkzJTpiXajQIwTXb_xekkmom2xpbIlbQlVxrwWAOJY62_DYUzNGxOQo0R4Gmp-bWsblubc9P85dhRH_XtEpt-YtTbquzKRQ7uVjvsF5xxXrduKcrF2w29kdpzNW3njLB2yFb8m4NsSm9iVhMdREnhz4WdLPxOf57yz-02KE8</recordid><startdate>19910301</startdate><enddate>19910301</enddate><creator>Staskus, Katherine A.</creator><creator>Retzel, Ernest F.</creator><creator>Lewis, Elizabeth D.</creator><creator>Silsby, Janet L.</creator><creator>Sheila Cyr, S.T.</creator><creator>Rank, Jeffrey M.</creator><creator>Wietgrefe, Steven W.</creator><creator>Haase, Ashley T.</creator><creator>Cook, Ronald</creator><creator>Fast, David</creator><creator>Geiser, Paul T.</creator><creator>Harty, John T.</creator><creator>Kong, Selene H.</creator><creator>Lahti, Carol J.</creator><creator>Neufeld, Thomas P.</creator><creator>Porter, Thomas E.</creator><creator>Shoop, Elizabeth</creator><creator>Zachow, Karen R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910301</creationdate><title>Isolation of replication-competent molecular clones of visna virus</title><author>Staskus, Katherine A. ; Retzel, Ernest F. ; Lewis, Elizabeth D. ; Silsby, Janet L. ; Sheila Cyr, S.T. ; Rank, Jeffrey M. ; Wietgrefe, Steven W. ; Haase, Ashley T. ; Cook, Ronald ; Fast, David ; Geiser, Paul T. ; Harty, John T. ; Kong, Selene H. ; Lahti, Carol J. ; Neufeld, Thomas P. ; Porter, Thomas E. ; Shoop, Elizabeth ; Zachow, Karen R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-b3dee46df60371cc1e063c0afcd47d77f03d36bf9aa888f8ee20d0e2b4e1cba23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Choroid Plexus</topic><topic>Chromosome Deletion</topic><topic>CLONE</topic><topic>CLONES</topic><topic>Cloning, Molecular</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>DESCENDANCE</topic><topic>DNA Transposable Elements</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>FENOTIPOS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>Open Reading Frames</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>PHENOTYPE</topic><topic>PROGENIE</topic><topic>Sheep</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><topic>Viral Plaque Assay</topic><topic>Virology</topic><topic>Virus Replication</topic><topic>VIRUS VISNA MAEDI</topic><topic>Visna-maedi virus - genetics</topic><topic>Visna-maedi virus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Staskus, Katherine A.</creatorcontrib><creatorcontrib>Retzel, Ernest F.</creatorcontrib><creatorcontrib>Lewis, Elizabeth D.</creatorcontrib><creatorcontrib>Silsby, Janet L.</creatorcontrib><creatorcontrib>Sheila Cyr, S.T.</creatorcontrib><creatorcontrib>Rank, Jeffrey M.</creatorcontrib><creatorcontrib>Wietgrefe, Steven W.</creatorcontrib><creatorcontrib>Haase, Ashley T.</creatorcontrib><creatorcontrib>Cook, Ronald</creatorcontrib><creatorcontrib>Fast, David</creatorcontrib><creatorcontrib>Geiser, Paul T.</creatorcontrib><creatorcontrib>Harty, John T.</creatorcontrib><creatorcontrib>Kong, Selene H.</creatorcontrib><creatorcontrib>Lahti, Carol J.</creatorcontrib><creatorcontrib>Neufeld, Thomas P.</creatorcontrib><creatorcontrib>Porter, Thomas E.</creatorcontrib><creatorcontrib>Shoop, Elizabeth</creatorcontrib><creatorcontrib>Zachow, Karen R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Staskus, Katherine A.</au><au>Retzel, Ernest F.</au><au>Lewis, Elizabeth D.</au><au>Silsby, Janet L.</au><au>Sheila Cyr, S.T.</au><au>Rank, Jeffrey M.</au><au>Wietgrefe, Steven W.</au><au>Haase, Ashley T.</au><au>Cook, Ronald</au><au>Fast, David</au><au>Geiser, Paul T.</au><au>Harty, John T.</au><au>Kong, Selene H.</au><au>Lahti, Carol J.</au><au>Neufeld, Thomas P.</au><au>Porter, Thomas E.</au><au>Shoop, Elizabeth</au><au>Zachow, Karen R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of replication-competent molecular clones of visna virus</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1991-03-01</date><risdate>1991</risdate><volume>181</volume><issue>1</issue><spage>228</spage><epage>240</epage><pages>228-240</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1847257</pmid><doi>10.1016/0042-6822(91)90488-W</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1991-03, Vol.181 (1), p.228-240 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animals ANTIGENE ANTIGENOS Base Sequence Biological and medical sciences Cells, Cultured Choroid Plexus Chromosome Deletion CLONE CLONES Cloning, Molecular CULTIVO DE CELULAS CULTURE DE CELLULE DESCENDANCE DNA Transposable Elements DNA, Viral - genetics DNA, Viral - isolation & purification FENOTIPOS Fundamental and applied biological sciences. Psychology Genes, Viral Genetics Microbiology Molecular Sequence Data NUCLEOTIDE NUCLEOTIDOS Open Reading Frames OVIN OVINOS PHENOTYPE PROGENIE Sheep VARIACION GENETICA VARIATION GENETIQUE Viral Plaque Assay Virology Virus Replication VIRUS VISNA MAEDI Visna-maedi virus - genetics Visna-maedi virus - physiology |
| title | Isolation of replication-competent molecular clones of visna virus |
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