Survival of Gardnerella vaginalis in human urine
The authors studied the survival of Gardnerella vaginalis in human urine and determined conditions for optimum recovery on agar media. Gardnerella counts declined by greater than 99.9% in urine held at 37 degrees C for 24 hours, whereas the falloff was negligible at 4 degrees C. Viability was lost a...
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| Veröffentlicht in: | American journal of clinical pathology 1991-02, Vol.95 (2), p.234-239 |
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| container_title | American journal of clinical pathology |
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| creator | MOY HEANG LAM BIRCH, D. F |
| description | The authors studied the survival of Gardnerella vaginalis in human urine and determined conditions for optimum recovery on agar media. Gardnerella counts declined by greater than 99.9% in urine held at 37 degrees C for 24 hours, whereas the falloff was negligible at 4 degrees C. Viability was lost after 6 hours in urine with pH of 5, and only 0.01% cells survived in urine with pH of 7. In contrast, greater than 90% cells survived exposure at pH of 6. Dialysis to remove small molecular weight (less than 14,000) inhibitors did not enhance survival. Co-cultivation with Ureaplasma urealyticum and the addition of glycogen improved survival. Maximum recovery from urine required anaerobic incubation on enriched agar medium (pH 6.5-7.5) for at least 48 hours. Gardnerella vaginalis survives poorly in human urine at 37 degrees C. Culture for these bacteria requires prolonged anaerobic incubation. |
| doi_str_mv | 10.1093/ajcp/95.2.234 |
| format | Article |
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F</creator><creatorcontrib>MOY HEANG LAM ; BIRCH, D. F</creatorcontrib><description>The authors studied the survival of Gardnerella vaginalis in human urine and determined conditions for optimum recovery on agar media. Gardnerella counts declined by greater than 99.9% in urine held at 37 degrees C for 24 hours, whereas the falloff was negligible at 4 degrees C. Viability was lost after 6 hours in urine with pH of 5, and only 0.01% cells survived in urine with pH of 7. In contrast, greater than 90% cells survived exposure at pH of 6. Dialysis to remove small molecular weight (less than 14,000) inhibitors did not enhance survival. Co-cultivation with Ureaplasma urealyticum and the addition of glycogen improved survival. Maximum recovery from urine required anaerobic incubation on enriched agar medium (pH 6.5-7.5) for at least 48 hours. Gardnerella vaginalis survives poorly in human urine at 37 degrees C. Culture for these bacteria requires prolonged anaerobic incubation.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/95.2.234</identifier><identifier>PMID: 1992615</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Agar ; Bacterial diseases ; Biological and medical sciences ; Culture Media ; Dialysis ; Female ; Gardnerella vaginalis - growth & development ; Gardnerella vaginalis - isolation & purification ; Glycogen - urine ; Humans ; Hydrogen-Ion Concentration ; Infectious diseases ; Medical sciences ; Temperature ; Ureaplasma - physiology ; Urine - microbiology</subject><ispartof>American journal of clinical pathology, 1991-02, Vol.95 (2), p.234-239</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-da201a413ae1a43aab642b87035963ccea5b975fd3665428b14f37939cf9da6c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5454671$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1992615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOY HEANG LAM</creatorcontrib><creatorcontrib>BIRCH, D. F</creatorcontrib><title>Survival of Gardnerella vaginalis in human urine</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>The authors studied the survival of Gardnerella vaginalis in human urine and determined conditions for optimum recovery on agar media. Gardnerella counts declined by greater than 99.9% in urine held at 37 degrees C for 24 hours, whereas the falloff was negligible at 4 degrees C. Viability was lost after 6 hours in urine with pH of 5, and only 0.01% cells survived in urine with pH of 7. In contrast, greater than 90% cells survived exposure at pH of 6. Dialysis to remove small molecular weight (less than 14,000) inhibitors did not enhance survival. Co-cultivation with Ureaplasma urealyticum and the addition of glycogen improved survival. Maximum recovery from urine required anaerobic incubation on enriched agar medium (pH 6.5-7.5) for at least 48 hours. Gardnerella vaginalis survives poorly in human urine at 37 degrees C. Culture for these bacteria requires prolonged anaerobic incubation.</description><subject>Agar</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Culture Media</subject><subject>Dialysis</subject><subject>Female</subject><subject>Gardnerella vaginalis - growth & development</subject><subject>Gardnerella vaginalis - isolation & purification</subject><subject>Glycogen - urine</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Temperature</subject><subject>Ureaplasma - physiology</subject><subject>Urine - microbiology</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAURS0EKqUwMiJlQGxpbT9_xCOqoCBVYgBm68VxwFWaFLupxL8nVSsYme5wj67eO4RcMzpl1MAMV24zM3LKpxzECRkzIyDXmvNTMqaU8twwDefkIqUVpYwXVIzIiBnDFZNjQl_7uAs7bLKuzhYYq9ZH3zSY7fAjtNiElIU2--zX2GZ9DK2_JGc1NslfHXNC3h8f3uZP-fJl8Ty_X-YORLHNK-SUoWCAfghALJXgZaEpSKPAOY-yNFrWFSglBS9KJmrQBoyrTYXKwYTcHXY3sfvqfdradUhuf1rruz7Z4Q8wXOh_QaaoMLSQA5gfQBe7lKKv7SaGNcZvy6jdq7R7ldZIy-2gcuBvjsN9ufbVH31wN_S3xx6Tw6aO2LqQfjEppFCawQ8dRXrL</recordid><startdate>19910201</startdate><enddate>19910201</enddate><creator>MOY HEANG LAM</creator><creator>BIRCH, D. F</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910201</creationdate><title>Survival of Gardnerella vaginalis in human urine</title><author>MOY HEANG LAM ; BIRCH, D. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-da201a413ae1a43aab642b87035963ccea5b975fd3665428b14f37939cf9da6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Agar</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Culture Media</topic><topic>Dialysis</topic><topic>Female</topic><topic>Gardnerella vaginalis - growth & development</topic><topic>Gardnerella vaginalis - isolation & purification</topic><topic>Glycogen - urine</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Temperature</topic><topic>Ureaplasma - physiology</topic><topic>Urine - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOY HEANG LAM</creatorcontrib><creatorcontrib>BIRCH, D. F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOY HEANG LAM</au><au>BIRCH, D. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survival of Gardnerella vaginalis in human urine</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>95</volume><issue>2</issue><spage>234</spage><epage>239</epage><pages>234-239</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>The authors studied the survival of Gardnerella vaginalis in human urine and determined conditions for optimum recovery on agar media. Gardnerella counts declined by greater than 99.9% in urine held at 37 degrees C for 24 hours, whereas the falloff was negligible at 4 degrees C. Viability was lost after 6 hours in urine with pH of 5, and only 0.01% cells survived in urine with pH of 7. In contrast, greater than 90% cells survived exposure at pH of 6. Dialysis to remove small molecular weight (less than 14,000) inhibitors did not enhance survival. Co-cultivation with Ureaplasma urealyticum and the addition of glycogen improved survival. Maximum recovery from urine required anaerobic incubation on enriched agar medium (pH 6.5-7.5) for at least 48 hours. Gardnerella vaginalis survives poorly in human urine at 37 degrees C. Culture for these bacteria requires prolonged anaerobic incubation.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>1992615</pmid><doi>10.1093/ajcp/95.2.234</doi><tpages>6</tpages></addata></record> |
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| ispartof | American journal of clinical pathology, 1991-02, Vol.95 (2), p.234-239 |
| issn | 0002-9173 1943-7722 |
| language | eng |
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| source | MEDLINE; Oxford Journals A-Z Collection |
| subjects | Agar Bacterial diseases Biological and medical sciences Culture Media Dialysis Female Gardnerella vaginalis - growth & development Gardnerella vaginalis - isolation & purification Glycogen - urine Humans Hydrogen-Ion Concentration Infectious diseases Medical sciences Temperature Ureaplasma - physiology Urine - microbiology |
| title | Survival of Gardnerella vaginalis in human urine |
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