Modification of the fluorescent allergosorbent test as an inhibition assay for determination of cross-reactivity among aeroallergens
The fluorescent allergosorbent test was adapted as an inhibition assay to determine cross-reactivity between aeroallergens. With this method, similar antigenic determinants were found between short ragweed and giant ragweed, cocklebur, lamb's-quarter, rough pigweed, marsh elder, and goldenrod....
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| Veröffentlicht in: | Journal of allergy and clinical immunology 1991, Vol.87 (1), p.98-103 |
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| container_end_page | 103 |
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| container_issue | 1 |
| container_start_page | 98 |
| container_title | Journal of allergy and clinical immunology |
| container_volume | 87 |
| creator | Perrick, David Stafford, Chester T. Armstrong, Elisa DuRant, Robert H. |
| description | The fluorescent allergosorbent test was adapted as an inhibition assay to determine cross-reactivity between aeroallergens. With this method, similar antigenic determinants were found between short ragweed and giant ragweed, cocklebur, lamb's-quarter, rough pigweed, marsh elder, and goldenrod. Cocklebur and giant ragweed were highly potent in their ability to competitively bind to short ragweed IgE. The other pollens demonstrated lower potency of cross-reacting antigens. The fluorescent allergosorbent test—inhibition assay appears to be a useful method to determine cross-reactivity among aeroallergens. |
| doi_str_mv | 10.1016/0091-6749(91)90218-D |
| format | Article |
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With this method, similar antigenic determinants were found between short ragweed and giant ragweed, cocklebur, lamb's-quarter, rough pigweed, marsh elder, and goldenrod. Cocklebur and giant ragweed were highly potent in their ability to competitively bind to short ragweed IgE. The other pollens demonstrated lower potency of cross-reacting antigens. 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| identifier | ISSN: 0091-6749 |
| ispartof | Journal of allergy and clinical immunology, 1991, Vol.87 (1), p.98-103 |
| issn | 0091-6749 1097-6825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80438654 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Allergological tests Binding, Competitive Biological and medical sciences Cross Reactions Epitopes - immunology Fluorescent Antibody Technique Humans Immunoglobulin E - metabolism Immunological methods for diagnosis and exploration Immunopathology Immunosorbent Techniques Medical sciences Pollen - immunology Rhinitis, Allergic, Seasonal - diagnosis |
| title | Modification of the fluorescent allergosorbent test as an inhibition assay for determination of cross-reactivity among aeroallergens |
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