Inhibition of tissue-type plasminogen activator by conditioned medium from cultured human and porcine vascular endothelial cells

Inhibition of tissue-type plasminogen activator by conditioned medium from cultured human and porcine vascular endothelial cells

Conditioned serum-free medium both from confluent porcine aortic endothelial cells and from human venous and arterial umbilical cord endothelial cells will inhibit plasminogen activation by tissue-type plasminogen activator. Inhibitory activity in the conditioned medium increases with time, and depe...

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Veröffentlicht in:Biochemical and biophysical research communications 1983-01, Vol.110 (2), p.392-398
Hauptverfasser: Emeis, J.J., van Hinsbergh, V.W.M., Verheijen, J.H., Wijngaards, G.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Aorta - metabolism
Blood Vessels - metabolism
Cells, Cultured
Culture Media
Endothelium - metabolism
Female
Humans
Plasminogen Activators - antagonists & inhibitors
Plasminogen Inactivators
Swine
Tissue Extracts - analysis
Umbilical Cord - metabolism
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container_end_page 398
container_issue 2
container_start_page 392
container_title Biochemical and biophysical research communications
container_volume 110
creator Emeis, J.J.
van Hinsbergh, V.W.M.
Verheijen, J.H.
Wijngaards, G.
description Conditioned serum-free medium both from confluent porcine aortic endothelial cells and from human venous and arterial umbilical cord endothelial cells will inhibit plasminogen activation by tissue-type plasminogen activator. Inhibitory activity in the conditioned medium increases with time, and depends upon protein synthesis. Also, the conditioned medium directly inhibits the amidolytic activity of tissue-type plasminogen activator. Inhibitory activity can be removed from conditioned medium by absorption with immobilized tissue-type plasminogen activator.
doi_str_mv 10.1016/0006-291X(83)91161-0
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subjects Animals
Aorta - metabolism
Blood Vessels - metabolism
Cells, Cultured
Culture Media
Endothelium - metabolism
Female
Humans
Plasminogen Activators - antagonists & inhibitors
Plasminogen Inactivators
Swine
Tissue Extracts - analysis
Umbilical Cord - metabolism
title Inhibition of tissue-type plasminogen activator by conditioned medium from cultured human and porcine vascular endothelial cells
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