Modulation of interleukin 2 internalization and interleukin 2-dependent cell growth by antireceptor antibodies
The growth factor interleukin 2 (IL2) binds to and is internalized together with high-affinity surface receptors present on lymphoid cells. This endocytosis thus results in down-regulation of the receptors. However, it is not known if the internalization is relevant to the induction of cell growth....
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| Veröffentlicht in: | The Journal of biological chemistry 1991-01, Vol.266 (3), p.1497-1501 |
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| container_issue | 3 |
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| container_title | The Journal of biological chemistry |
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| creator | Duprez, V Ferrer, M Cornet, V Olive, D Dautry-Varsat, A |
| description | The growth factor interleukin 2 (IL2) binds to and is internalized together with high-affinity surface receptors present on
lymphoid cells. This endocytosis thus results in down-regulation of the receptors. However, it is not known if the internalization
is relevant to the induction of cell growth. In the present study a rat monoclonal antibody to the P55 chain of the IL2 receptor
was used to examine the role of receptor internalization in the IL2-dependent autocrine human tumor T cell line IARC 301.
When given alone, this antibody did not inhibit IL2 binding, internalization, or IL2-dependent cell proliferation. However,
crosslinking by anti-rat immunoglobulins, which did not affect binding of the growth factor, inhibited both IL2 internalization
and cell proliferation. Besides offering a novel means for the specific inhibition of the uptake of IL2 bound to IL2 high-affinity
receptors, the results are compatible with the association of this receptor-ligand uptake to the growth stimulation by IL2. |
| doi_str_mv | 10.1016/S0021-9258(18)52322-X |
| format | Article |
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lymphoid cells. This endocytosis thus results in down-regulation of the receptors. However, it is not known if the internalization
is relevant to the induction of cell growth. In the present study a rat monoclonal antibody to the P55 chain of the IL2 receptor
was used to examine the role of receptor internalization in the IL2-dependent autocrine human tumor T cell line IARC 301.
When given alone, this antibody did not inhibit IL2 binding, internalization, or IL2-dependent cell proliferation. However,
crosslinking by anti-rat immunoglobulins, which did not affect binding of the growth factor, inhibited both IL2 internalization
and cell proliferation. Besides offering a novel means for the specific inhibition of the uptake of IL2 bound to IL2 high-affinity
receptors, the results are compatible with the association of this receptor-ligand uptake to the growth stimulation by IL2.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)52322-X</identifier><identifier>PMID: 1988432</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Antibodies, Monoclonal ; Biological and medical sciences ; Cell Division ; Cell Line ; Cell physiology ; Down-Regulation ; Endocytosis ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunologic Capping ; In Vitro Techniques ; Interleukin-2 - physiology ; Molecular and cellular biology ; Receptor Aggregation ; Receptors, Interleukin-2 - immunology ; Receptors, Interleukin-2 - physiology ; Responses to growth factors, tumor promotors, other factors ; T-Lymphocytes - cytology ; Time Factors</subject><ispartof>The Journal of biological chemistry, 1991-01, Vol.266 (3), p.1497-1501</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-bb341f872318531d9663553da8d22a506c5c4934cd14745a50b542b7098f92363</citedby><cites>FETCH-LOGICAL-c439t-bb341f872318531d9663553da8d22a506c5c4934cd14745a50b542b7098f92363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19623431$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1988432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duprez, V</creatorcontrib><creatorcontrib>Ferrer, M</creatorcontrib><creatorcontrib>Cornet, V</creatorcontrib><creatorcontrib>Olive, D</creatorcontrib><creatorcontrib>Dautry-Varsat, A</creatorcontrib><title>Modulation of interleukin 2 internalization and interleukin 2-dependent cell growth by antireceptor antibodies</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The growth factor interleukin 2 (IL2) binds to and is internalized together with high-affinity surface receptors present on
lymphoid cells. This endocytosis thus results in down-regulation of the receptors. However, it is not known if the internalization
is relevant to the induction of cell growth. In the present study a rat monoclonal antibody to the P55 chain of the IL2 receptor
was used to examine the role of receptor internalization in the IL2-dependent autocrine human tumor T cell line IARC 301.
When given alone, this antibody did not inhibit IL2 binding, internalization, or IL2-dependent cell proliferation. However,
crosslinking by anti-rat immunoglobulins, which did not affect binding of the growth factor, inhibited both IL2 internalization
and cell proliferation. Besides offering a novel means for the specific inhibition of the uptake of IL2 bound to IL2 high-affinity
receptors, the results are compatible with the association of this receptor-ligand uptake to the growth stimulation by IL2.</description><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Down-Regulation</subject><subject>Endocytosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunologic Capping</subject><subject>In Vitro Techniques</subject><subject>Interleukin-2 - physiology</subject><subject>Molecular and cellular biology</subject><subject>Receptor Aggregation</subject><subject>Receptors, Interleukin-2 - immunology</subject><subject>Receptors, Interleukin-2 - physiology</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>T-Lymphocytes - cytology</subject><subject>Time Factors</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhoO01LX6EwqDYKkXozn5muSylFaFll6osHchk2S60dlkTWYo7a_v7M6i9aq5Ceec53zwvgidAP4EGMTn7xgTqBXh8gzkR04oIfXyFVoAlrSmHJYHaPEXeY3elPILT48pOEJHoKRklCxQvElu7M0QUqxSV4U4-Nz78XeIFZmjaPrwOAMmuv-J2vmNj87HobK-76u7nO6HVdU-TOgQsrd-M6S8C9rkgi9v0WFn-uLf7f9j9PPq8sfF1_r69su3i_Pr2jKqhrptKYNONoSC5BScEoJyTp2RjhDDsbDcMkWZdcAaxqdMyxlpG6xkpwgV9BidznM3Of0ZfRn0OpTtiSb6NBYtMSPNVqmXQOCyEYThCeQzaHMqJftOb3JYm_ygAeutIXpniN6qrUHqnSF6OfWd7BeM7dq7f12zA1P9w75uijV9l020oTzDBKGMwsS9n7lVuFvdT9rqNiS78mtNhNBUA1MNfQINyJ8U</recordid><startdate>19910125</startdate><enddate>19910125</enddate><creator>Duprez, V</creator><creator>Ferrer, M</creator><creator>Cornet, V</creator><creator>Olive, D</creator><creator>Dautry-Varsat, A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910125</creationdate><title>Modulation of interleukin 2 internalization and interleukin 2-dependent cell growth by antireceptor antibodies</title><author>Duprez, V ; Ferrer, M ; Cornet, V ; Olive, D ; Dautry-Varsat, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-bb341f872318531d9663553da8d22a506c5c4934cd14745a50b542b7098f92363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Down-Regulation</topic><topic>Endocytosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunologic Capping</topic><topic>In Vitro Techniques</topic><topic>Interleukin-2 - physiology</topic><topic>Molecular and cellular biology</topic><topic>Receptor Aggregation</topic><topic>Receptors, Interleukin-2 - immunology</topic><topic>Receptors, Interleukin-2 - physiology</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>T-Lymphocytes - cytology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duprez, V</creatorcontrib><creatorcontrib>Ferrer, M</creatorcontrib><creatorcontrib>Cornet, V</creatorcontrib><creatorcontrib>Olive, D</creatorcontrib><creatorcontrib>Dautry-Varsat, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duprez, V</au><au>Ferrer, M</au><au>Cornet, V</au><au>Olive, D</au><au>Dautry-Varsat, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of interleukin 2 internalization and interleukin 2-dependent cell growth by antireceptor antibodies</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1991-01-25</date><risdate>1991</risdate><volume>266</volume><issue>3</issue><spage>1497</spage><epage>1501</epage><pages>1497-1501</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The growth factor interleukin 2 (IL2) binds to and is internalized together with high-affinity surface receptors present on
lymphoid cells. This endocytosis thus results in down-regulation of the receptors. However, it is not known if the internalization
is relevant to the induction of cell growth. In the present study a rat monoclonal antibody to the P55 chain of the IL2 receptor
was used to examine the role of receptor internalization in the IL2-dependent autocrine human tumor T cell line IARC 301.
When given alone, this antibody did not inhibit IL2 binding, internalization, or IL2-dependent cell proliferation. However,
crosslinking by anti-rat immunoglobulins, which did not affect binding of the growth factor, inhibited both IL2 internalization
and cell proliferation. Besides offering a novel means for the specific inhibition of the uptake of IL2 bound to IL2 high-affinity
receptors, the results are compatible with the association of this receptor-ligand uptake to the growth stimulation by IL2.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1988432</pmid><doi>10.1016/S0021-9258(18)52322-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1991-01, Vol.266 (3), p.1497-1501 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antibodies, Monoclonal Biological and medical sciences Cell Division Cell Line Cell physiology Down-Regulation Endocytosis Fundamental and applied biological sciences. Psychology Humans Immunologic Capping In Vitro Techniques Interleukin-2 - physiology Molecular and cellular biology Receptor Aggregation Receptors, Interleukin-2 - immunology Receptors, Interleukin-2 - physiology Responses to growth factors, tumor promotors, other factors T-Lymphocytes - cytology Time Factors |
| title | Modulation of interleukin 2 internalization and interleukin 2-dependent cell growth by antireceptor antibodies |
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