Gene cluster rpoBC1C2 in cyanobacteria does not constitute an operon
The core enzyme of the cyanobacterial DNA-dependent RNA polymerase contains a unique component, γ, which is absent from the corresponding enzymes of other eubacteria. In the heterocystous cyanobacterium Nostoc commune the gene encoding γ, rpoC1, is immediately adjacent to, and downstream of, rpoB. T...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1991, Vol.284 (1), p.22-25 |
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| creator | Xie, WenQin Potts, Malcolm |
| description | The core enzyme of the cyanobacterial DNA-dependent RNA polymerase contains a unique component, γ, which is absent from the corresponding enzymes of other eubacteria. In the heterocystous cyanobacterium
Nostoc commune the gene encoding γ,
rpoC1, is immediately adjacent to, and downstream of,
rpoB. The
rpoC1 gene, and a 3′ adjacent gene,
rpoC2, correspond to the single
rpoC gene found in
Escherichia coli with respect to those domains conserved within their translational products. Northern analysis and primer extension assay show that in
N. commune,
rpoC1 and
rpoC2 are transcribed separately from
rpoB. The promoter of
rpoC1C2 can direct the expression of a promotorless
lacZ gene in
E. coli. As a consequence, cyanobacterial
rpo gene expression is distinct from the mode of cotranscription described for the equivalent sequences found in other eubacteria, archaebacteria, and plant chloroplasts. Also in this paper, a simple protocol for RNA isolation, which should be applicable for RNA isolation from plant cells, is presented. |
| doi_str_mv | 10.1016/0003-9861(91)90256-I |
| format | Article |
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Nostoc commune the gene encoding γ,
rpoC1, is immediately adjacent to, and downstream of,
rpoB. The
rpoC1 gene, and a 3′ adjacent gene,
rpoC2, correspond to the single
rpoC gene found in
Escherichia coli with respect to those domains conserved within their translational products. Northern analysis and primer extension assay show that in
N. commune,
rpoC1 and
rpoC2 are transcribed separately from
rpoB. The promoter of
rpoC1C2 can direct the expression of a promotorless
lacZ gene in
E. coli. As a consequence, cyanobacterial
rpo gene expression is distinct from the mode of cotranscription described for the equivalent sequences found in other eubacteria, archaebacteria, and plant chloroplasts. Also in this paper, a simple protocol for RNA isolation, which should be applicable for RNA isolation from plant cells, is presented.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(91)90256-I</identifier><identifier>PMID: 1899176</identifier><identifier>CODEN: ABBIA4</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cyanobacteria - genetics ; DNA-Directed RNA Polymerases - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Genes, Plant ; Hydrogen Bonding ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecular Structure ; Nostoc commune ; Oligonucleotides - chemistry ; Operon ; Plant Proteins - genetics ; Promoter Regions, Genetic ; Regulatory Sequences, Nucleic Acid ; RNA, Messenger - genetics ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Archives of biochemistry and biophysics, 1991, Vol.284 (1), p.22-25</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-bf35cb40356605e3abfc5bf3934ec487e6021167b61aa8b7f8607f025a9f08f23</citedby><cites>FETCH-LOGICAL-c418t-bf35cb40356605e3abfc5bf3934ec487e6021167b61aa8b7f8607f025a9f08f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000398619190256I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19495266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1899176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, WenQin</creatorcontrib><creatorcontrib>Potts, Malcolm</creatorcontrib><title>Gene cluster rpoBC1C2 in cyanobacteria does not constitute an operon</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The core enzyme of the cyanobacterial DNA-dependent RNA polymerase contains a unique component, γ, which is absent from the corresponding enzymes of other eubacteria. In the heterocystous cyanobacterium
Nostoc commune the gene encoding γ,
rpoC1, is immediately adjacent to, and downstream of,
rpoB. The
rpoC1 gene, and a 3′ adjacent gene,
rpoC2, correspond to the single
rpoC gene found in
Escherichia coli with respect to those domains conserved within their translational products. Northern analysis and primer extension assay show that in
N. commune,
rpoC1 and
rpoC2 are transcribed separately from
rpoB. The promoter of
rpoC1C2 can direct the expression of a promotorless
lacZ gene in
E. coli. As a consequence, cyanobacterial
rpo gene expression is distinct from the mode of cotranscription described for the equivalent sequences found in other eubacteria, archaebacteria, and plant chloroplasts. Also in this paper, a simple protocol for RNA isolation, which should be applicable for RNA isolation from plant cells, is presented.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cyanobacteria - genetics</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Genes, Plant</subject><subject>Hydrogen Bonding</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Nostoc commune</subject><subject>Oligonucleotides - chemistry</subject><subject>Operon</subject><subject>Plant Proteins - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>Gene Expression Regulation</topic><topic>Genes, Plant</topic><topic>Hydrogen Bonding</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecular Structure</topic><topic>Nostoc commune</topic><topic>Oligonucleotides - chemistry</topic><topic>Operon</topic><topic>Plant Proteins - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, WenQin</creatorcontrib><creatorcontrib>Potts, Malcolm</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, WenQin</au><au>Potts, Malcolm</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene cluster rpoBC1C2 in cyanobacteria does not constitute an operon</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1991</date><risdate>1991</risdate><volume>284</volume><issue>1</issue><spage>22</spage><epage>25</epage><pages>22-25</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><coden>ABBIA4</coden><abstract>The core enzyme of the cyanobacterial DNA-dependent RNA polymerase contains a unique component, γ, which is absent from the corresponding enzymes of other eubacteria. In the heterocystous cyanobacterium
Nostoc commune the gene encoding γ,
rpoC1, is immediately adjacent to, and downstream of,
rpoB. The
rpoC1 gene, and a 3′ adjacent gene,
rpoC2, correspond to the single
rpoC gene found in
Escherichia coli with respect to those domains conserved within their translational products. Northern analysis and primer extension assay show that in
N. commune,
rpoC1 and
rpoC2 are transcribed separately from
rpoB. The promoter of
rpoC1C2 can direct the expression of a promotorless
lacZ gene in
E. coli. As a consequence, cyanobacterial
rpo gene expression is distinct from the mode of cotranscription described for the equivalent sequences found in other eubacteria, archaebacteria, and plant chloroplasts. Also in this paper, a simple protocol for RNA isolation, which should be applicable for RNA isolation from plant cells, is presented.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1899176</pmid><doi>10.1016/0003-9861(91)90256-I</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1991, Vol.284 (1), p.22-25 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80426531 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Base Sequence Biological and medical sciences Blotting, Northern Cyanobacteria - genetics DNA-Directed RNA Polymerases - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, Plant Hydrogen Bonding Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Structure Nostoc commune Oligonucleotides - chemistry Operon Plant Proteins - genetics Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid RNA, Messenger - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | Gene cluster rpoBC1C2 in cyanobacteria does not constitute an operon |
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