Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein
Research Institute for Viral Preparations, Moscow, U.S.S.R. A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear...
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| Veröffentlicht in: | Journal of general virology 1983-02, Vol.64 (2), p.291-304 |
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| container_title | Journal of general virology |
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| creator | Ghendon, Y. Z Markushin, S. G Klimov, A. I Lotte, V. D Ginzburg, V. P |
| description | Research Institute for Viral Preparations, Moscow, U.S.S.R.
A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP:M ratio as well as a decreased haemagglutinin content. A population of virions formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.
Keywords: orthomyxoviruses, fowl plague virus, ts mutants, M protein
Received 22 April 1982;
accepted 31 August 1982. |
| doi_str_mv | 10.1099/0022-1317-64-2-291 |
| format | Article |
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A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP:M ratio as well as a decreased haemagglutinin content. A population of virions formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.
Keywords: orthomyxoviruses, fowl plague virus, ts mutants, M protein
Received 22 April 1982;
accepted 31 August 1982.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-64-2-291</identifier><identifier>PMID: 6834002</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Genes, Viral ; Genetic Code ; Genetic Complementation Test ; Hemagglutinins, Viral - genetics ; Influenza A virus - genetics ; Microscopy, Electron ; Mutation ; Neuraminidase - genetics ; RNA, Viral - genetics ; Temperature ; Viral Matrix Proteins ; Viral Proteins - genetics ; Virion - genetics</subject><ispartof>Journal of general virology, 1983-02, Vol.64 (2), p.291-304</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ccde654b61449b4d4d8c859f39fa3a1797b8edad674abf81de2610d4ed2bbb9e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6834002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghendon, Y. Z</creatorcontrib><creatorcontrib>Markushin, S. G</creatorcontrib><creatorcontrib>Klimov, A. I</creatorcontrib><creatorcontrib>Lotte, V. D</creatorcontrib><creatorcontrib>Ginzburg, V. P</creatorcontrib><title>Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Research Institute for Viral Preparations, Moscow, U.S.S.R.
A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP:M ratio as well as a decreased haemagglutinin content. A population of virions formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.
Keywords: orthomyxoviruses, fowl plague virus, ts mutants, M protein
Received 22 April 1982;
accepted 31 August 1982.</description><subject>Genes, Viral</subject><subject>Genetic Code</subject><subject>Genetic Complementation Test</subject><subject>Hemagglutinins, Viral - genetics</subject><subject>Influenza A virus - genetics</subject><subject>Microscopy, Electron</subject><subject>Mutation</subject><subject>Neuraminidase - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Temperature</subject><subject>Viral Matrix Proteins</subject><subject>Viral Proteins - genetics</subject><subject>Virion - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo67j6BQQhJz1F86_T3UcZ3F1hxQVXryHpVM9EupMxSc_itzfNDHP1VIf3e6-Kegi9ZfQjo33_iVLOCROsJUoSTnjPnqENk6ohvMrP0eYCvESvcv5NKZOyaa_QleqErOIG5R9lcR4yjgEb_AjzAZIpSwKSIWRf_BHwt6WYUHAc8U18mvDDZHYL4F8-LRnfmaMPu2pdoeJrig_4FgLgFm-jW7UxJlz2NQY_pFjAh9foxWimDG_O8xr9vPnyuL0j999vv24_35NB0qaQYXCgGmlVPbq30knXDV3Tj6IfjTCs7VvbgTNOtdLYsWMOuGLUSXDcWtuDuEbvT7mHFP8skIuefR5gmkyAuGTdUcmZoPy_IGsaJZQQFeQncEgx5wSjPiQ_m_RXM6rXSvT6cb1-XCupua6VVNO7c_piZ3AXy7mDqn846Xu_2z_5BHoHYfZ1hfVRH326JP0DpISU8Q</recordid><startdate>198302</startdate><enddate>198302</enddate><creator>Ghendon, Y. Z</creator><creator>Markushin, S. G</creator><creator>Klimov, A. I</creator><creator>Lotte, V. D</creator><creator>Ginzburg, V. P</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>198302</creationdate><title>Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein</title><author>Ghendon, Y. Z ; Markushin, S. G ; Klimov, A. I ; Lotte, V. D ; Ginzburg, V. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ccde654b61449b4d4d8c859f39fa3a1797b8edad674abf81de2610d4ed2bbb9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Genes, Viral</topic><topic>Genetic Code</topic><topic>Genetic Complementation Test</topic><topic>Hemagglutinins, Viral - genetics</topic><topic>Influenza A virus - genetics</topic><topic>Microscopy, Electron</topic><topic>Mutation</topic><topic>Neuraminidase - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Temperature</topic><topic>Viral Matrix Proteins</topic><topic>Viral Proteins - genetics</topic><topic>Virion - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghendon, Y. Z</creatorcontrib><creatorcontrib>Markushin, S. G</creatorcontrib><creatorcontrib>Klimov, A. I</creatorcontrib><creatorcontrib>Lotte, V. D</creatorcontrib><creatorcontrib>Ginzburg, V. P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghendon, Y. Z</au><au>Markushin, S. G</au><au>Klimov, A. I</au><au>Lotte, V. D</au><au>Ginzburg, V. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1983-02</date><risdate>1983</risdate><volume>64</volume><issue>2</issue><spage>291</spage><epage>304</epage><pages>291-304</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Research Institute for Viral Preparations, Moscow, U.S.S.R.
A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP:M ratio as well as a decreased haemagglutinin content. A population of virions formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.
Keywords: orthomyxoviruses, fowl plague virus, ts mutants, M protein
Received 22 April 1982;
accepted 31 August 1982.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>6834002</pmid><doi>10.1099/0022-1317-64-2-291</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1983-02, Vol.64 (2), p.291-304 |
| issn | 0022-1317 1465-2099 |
| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Genes, Viral Genetic Code Genetic Complementation Test Hemagglutinins, Viral - genetics Influenza A virus - genetics Microscopy, Electron Mutation Neuraminidase - genetics RNA, Viral - genetics Temperature Viral Matrix Proteins Viral Proteins - genetics Virion - genetics |
| title | Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein |
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