Uptake and release of protons during the reaction between cytochrome c oxidase and molecular oxygen: a flow-flash investigation
Changes in pH during the reactions of the fully reduced and mixed-valence cytochrome oxidase with molecular oxygen have been followed in flow-flash experiments, using the pH indicator phenol red. Solubilized enzyme as well as enzyme reconstituted into phospholipid vesicles has been studied. With the...
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| Veröffentlicht in: | Biochemistry (Easton) 1991-01, Vol.30 (2), p.436-440 |
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| container_title | Biochemistry (Easton) |
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| creator | Oliveberg, Mikael Hallen, Stefan Nilsson, Thomas |
| description | Changes in pH during the reactions of the fully reduced and mixed-valence cytochrome oxidase with molecular oxygen have been followed in flow-flash experiments, using the pH indicator phenol red. Solubilized enzyme as well as enzyme reconstituted into phospholipid vesicles has been studied. With the solubilized enzyme, a biphasic uptake of one proton from the medium was observed, whereas the reconstituted enzyme gave release of 1.3 protons to the extravesicular medium. It is concluded from these results that a total of two to three protons are taken up during oxidation of the fully reduced enzyme. Kinetic analysis suggests that the proton uptake is initiated by the transfer of the third electron to the oxygen binding site. A reaction scheme that integrates proton transfers and oxygen chemistry is presented. |
| doi_str_mv | 10.1021/bi00216a019 |
| format | Article |
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A reaction scheme that integrates proton transfers and oxygen chemistry is presented.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00216a019</identifier><identifier>PMID: 1846296</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cattle ; Electron Transport Complex IV - chemistry ; Electron Transport Complex IV - metabolism ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. 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Solubilized enzyme as well as enzyme reconstituted into phospholipid vesicles has been studied. With the solubilized enzyme, a biphasic uptake of one proton from the medium was observed, whereas the reconstituted enzyme gave release of 1.3 protons to the extravesicular medium. It is concluded from these results that a total of two to three protons are taken up during oxidation of the fully reduced enzyme. Kinetic analysis suggests that the proton uptake is initiated by the transfer of the third electron to the oxygen binding site. A reaction scheme that integrates proton transfers and oxygen chemistry is presented.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Electron Transport Complex IV - chemistry</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases</topic><topic>oxygen</topic><topic>Oxygen - metabolism</topic><topic>Phospholipids</topic><topic>Photochemistry</topic><topic>Protons</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveberg, Mikael</creatorcontrib><creatorcontrib>Hallen, Stefan</creatorcontrib><creatorcontrib>Nilsson, Thomas</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveberg, Mikael</au><au>Hallen, Stefan</au><au>Nilsson, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake and release of protons during the reaction between cytochrome c oxidase and molecular oxygen: a flow-flash investigation</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1991-01-15</date><risdate>1991</risdate><volume>30</volume><issue>2</issue><spage>436</spage><epage>440</epage><pages>436-440</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Changes in pH during the reactions of the fully reduced and mixed-valence cytochrome oxidase with molecular oxygen have been followed in flow-flash experiments, using the pH indicator phenol red. Solubilized enzyme as well as enzyme reconstituted into phospholipid vesicles has been studied. With the solubilized enzyme, a biphasic uptake of one proton from the medium was observed, whereas the reconstituted enzyme gave release of 1.3 protons to the extravesicular medium. It is concluded from these results that a total of two to three protons are taken up during oxidation of the fully reduced enzyme. Kinetic analysis suggests that the proton uptake is initiated by the transfer of the third electron to the oxygen binding site. A reaction scheme that integrates proton transfers and oxygen chemistry is presented.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>1846296</pmid><doi>10.1021/bi00216a019</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1991-01, Vol.30 (2), p.436-440 |
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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Electron Transport Complex IV - chemistry Electron Transport Complex IV - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology In Vitro Techniques Kinetics Oxidation-Reduction Oxidoreductases oxygen Oxygen - metabolism Phospholipids Photochemistry Protons Solubility |
| title | Uptake and release of protons during the reaction between cytochrome c oxidase and molecular oxygen: a flow-flash investigation |
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