Development of sensitive immunoassays for detection of antibodies against hepatitis B surface antigen

Three micro solid phase immunoassays (a micro-SPRIA and two ELISA techniques) were developed and tested for the detection of anti-HBs antibodies. Two different crosslinkers (glutaraldehyde and N-succinimidyl 3-(2-pyridyldithio) propionate) were used to couple a goat anti-mouse IgG reagent to alkalin...

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Veröffentlicht in:	Journal of virological methods 1983, Vol.6 (1), p.41-52
Hauptverfasser:	Irina, Ionescu-Matiu, Sanchez, Yanuario, Fields, Howard A., Dreesman, Gordon R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase Animals anti-HBs Antibodies, Viral - analysis Cross-Linking Reagents ELISA Enzyme-Linked Immunosorbent Assay Evaluation Studies as Topic Glutaral Hepatitis B Antibodies - analysis Hepatitis B Surface Antigens - immunology Immunoenzyme Techniques Mice Mice, Inbred BALB C Radioimmunoassay - methods solid phase immunoassays Succinimides
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